Expression Construct Name: pCS2P+-wnt8 ORF2 CS2P+

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Vector: CS2P+

ORF2 amplified from pBand 3-5 ligated into BamHI-StuI cut CS2P+.
For Sense RNA: cut with Asp718 I and transcribe with SP6 polymerase.


Plasmid History
Species of Sequence Origin: Zebrafish