Vector: SP64T
Insert: EcoR1 insert blunted into Bgl II blunt of SP64T
Insert size: 3.23
Cloning sites: Bgl II, EcoR1
Transcription: linearize with Bam H1 and transcribe with SP6.
Notes for Use:
DH5-alpha host strain
Amp resistant SP64T
Citation: Christian et al., Development 111, 1045 (1991)