Expression Construct Name:
Xwnt-5A Hind RNA

RETURN TO XENOPUS EXPRESSION LIST

Vector: SP64T
Insert: oocyte cDNA from Melton lambda gt10 library
Insert size: 1526 bp
Cloning site: Hind III RF blunted into Bgl II site
Transcription: linearize with Xba1 and transcribe with SP6.
Sense: plasmid #3 on 5/22/90
Antisense: plasmid #2 on 5/22/90

Plasmid History
Constructed by Randall Moon
Date of Plasmid and by whom: Randall Moon, 4/9/90
Species of Sequence Origin: Xenopus

Notes for Use:
lambda G6 Hind III RF in pGEM4 Blue (3/19/90) isolated and blunted into SP64T.

Note: Hind III cuts 9aa 5' of a met which is used for translation, residing in putative leader sequence. This Hind III site is 13 aa 3' of actual initiation met. Removal of 5' UTR and the amino terminal 13 aa's yield a more active Xwnt, but the assumption is that using the second met, and the half leader sequence yield a wild type wnt after cleavage of the shortened leader.

Citation: Moon et al., Development 119, 97 (1993)