Vector: pSP64T
Insert: Xwnt-4
Insert size: ~1 Kb
Cloning site: PCR Primers
Transcription: linearize with Sal1 and transcribe with SP6.
Notes for Use:
See Book 2, p. 55 & p. 75 for complete cloning strategy. Basically had to use PCR & primers to reconstruct AUG, TAG codons containing Bgl II sites to clone into pSP64T.
Early clone name: pX4.2