Expression Construct Name: Delta Beta-catenin in GFP

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Plasmid size: 6647 bp
To make RNA, cut with No1 I and transcribe with SP6.

Plasmid History
Constructed by Randall Moon
Species of Sequence Origin: Xenopus laevis

Note For Use
Xenopus beta-catenin PCR amplified from st. 19 RNA. These PCR fragments have the first 138 bp after the AUG removed to eliminate a possible GSK-3 sequence. A new AUG has been inserted just after the Bam site. The mutant GFP of Hiem et al., inserted into Cla I site, followed by a STOP codon. This is no myc epitope.

Reference: Moon, 1997, unpublished.