Expression Construct Name: dnWnt-1

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Plasmid name: dnWnt-1
Plasmid size: 5010 bp
Vector: 4095
Insert: 915

Plasmid History
Constructed by Jeff Brown on date of 8/8/95
Species of Sequence Origin: Mouse

Notes for Use:
Transcription: For sense, linearize with Asp718 (BMB) and transcribe with SP6.
Comments: PCR amplification from clone 286 (McMahon and Moon). Also referred to as int-1 spnfc or int-1 spnfcD in notebook #3. The Wnt-1 gene is trunkated after the SPNFC peptide sequence.

Ref: Hoppler et al., Genes & Development, 10:2805-2817, 1996.