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Description: Most of the 3'UTR was removed to reduce RNA toxicity. Insert size ~2kb. The plasmid does not grow well in bacteria.
Notes for Use: Select clones with amp. For in vitro transcription linearize with Not1 and transcribe with SP6
Reference: Clone provided by Peter Klein, made by Matt Deardorff, based on clone described by DL Shi et al., MOD 10: 35 (1998)