RETURN TO XENOPUS EXPRESSION LIST
Constructed by: Julia Yang-Snyder (subclone), Jeff Brown (tags), 4/19/96
Notes for Use:
Comments: Rfz1 from Henk Roelink. This sequence has a few differences from the published sequence. Sites listed in Rfz1 are not a complete list and I haven't searched through the CS2+ sequence to see if/where those sites are present. MYC tag version made by Jeff Brown (2/98-3/98) insert the epitope into the StyI site (1904). The MYC epitope has a BglII site in it. The 3' Sty site is destroyed by the tags, but the 5' site is intact. Epitope can be dected in PBS/Formaldehyde fixed tissue permeabilzed with 0.2% Triton. The Myc tagged Rfz1 has been IP'd from a TBS/1% Triton buffer. Frizzled tends to ppt out of SDS sample buffer when boiled, so either warm samples to ~70 degrees Celsius for a few minutes before loading samples or use a strong denaturing loading buffer and incubate the samples at room temp for half and hour before loading. The StyI site is in the 3rd extracellular loop of the protein.