Probe Construct Name: pXOT30.1

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Vector: pBSSK
Insert: Xenopus cDNA (otxA)
Insert size: 2.3 Kb
Antisense probe cut with Not1 and transcribe with T7.
Sense probe cut with Hind III and transcribe with T3.


Plasmid History
Constructed by Scott Stachel at U.C. Berkeley, Harland lab
Species of Sequence Origin: Xenopus

Notes for Use:
Lights up anterior/dorsal ectoderm early, the anterior field.
From stage 30 onwards lights up fore and midbrain, retina.

in situ image