Vector: pBSSK
Insert: Xenopus cDNA (otxA)
Insert size: 2.3 Kb
Antisense probe cut with Not1 and transcribe with T7.
Sense probe cut with Hind III and transcribe with T3.
Notes for Use:
Lights up anterior/dorsal ectoderm early, the anterior field.
From stage 30 onwards lights up fore and midbrain, retina.