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Description: This is a CONTROL luciferase reporter with the TCF/LEF sites of Super8XFOPflash (construct M50) being mutated.
This construct was made by Ajamete Kaykas in the Moon lab. The backbone is the pTA-Luc vector of Clontech, which provides a minimal TA viral promoter driving expression of the firefly luciferase gene (see company publications for details). 8 TCF/LEF mutant binding sites were cloned into the Mlu1 site of this vector (8 copies of: AGGCCAAAGGgggta, with mutant TCF/LEF binding site in CAP letters, and a spacer in lower case, separating each copy of the mutant TCF/LEF site).
Notes for Use: Select clones with amp.
Reference: M. Veeman et al., Current Biology 13:680 (2003)