Mougous Lab

University of Washington



Ross BD, Vester AJ, Radey MC, Schmidtke DT, Pope CE, Hoffman LR, Hajjar A, Peterson SB,  Borenstein E, Mougous JD (2019) Human gut bacteria contain acquired interbacterial defence systems. Nature doi:10.1038/s41586-019-1708-z. 

Ting S, Bosch DE, Mangiameli SM, Radey MC, Huang S, Park Y, Kelly KA, Filip SK, Goo YA, Eng JK, Allaire M, Veesler D, Wiggins PA, Peterson SB, Mougous JD (2018) Bifunctional immunity proteins protect bacteria against FtsZ-targeting ADP-ribosylating toxins. Cell 175, 1–13.

Quentin D, Ahmad S, Shanthamoorthy P, Mougous JD, Whitney JC, Raunser S. Mechanism of loading and translocation of type VI secretion system effector Tse6. (2018) Nature Microbiology 3: 1142-1152.

Ledvina HE,  Kelly KA, Eshraghi A, Plemel RL, Peterson SB, Lee B, Steele S, Adler M, Kawula TH, Merz AJ, Skerrett SJ, Celli J, Mougous JD (2018) Phosphatidylinositol 3-kinase effector alters phagosomal maturation to promote intracellular growth of Francisella. Cell Host Microbe 24, 1–11.

Ross B, Hayes B, Radey MC, Lee X, Josek T, Bjork J, Neitzel D, Paskewitz S, Chou S, Mougous JD (2018) Ixodes scapularis does not harbor a stable midgut microbiome. The ISME Journal 10.1038/s41396-018-0161-6.

LaCourse KD, Peterson SB, Kulasekara HD, Radey MC, Kim J, Mougous JD (2018) Conditional toxicity and synergy drive diversity among antibacterial effectors. Nature Microbiology 3: 440-446.

Verster A, Ross B, Radey M, Bao Y, Goodman A, Mougous JD, Borenstein E (2017) The landscape of type VI secretion across human gut microbiomes reveals its role in community composition. Cell Host Microbe 22(3): 411–419.

Whitney JC, Peterson SB, Kim J, Pazos M, Verster AJ, Radey MC, Kulasekara HD, Ching MQ, Bullen NP, Bryant D, Goo YA, Surette MG, Borenstein E, Vollmer W, Mougous JD (2017) A broadly distributed toxin family mediates contact-dependent antagonism between gram-positive bacteria. eLife 10.7554/eLife.26938.

Hood RD, Peterson SB, Mougous JD (2017) From striking out to striking gold: discovering that type VI secretion targets bacteria. Cell Host Microbe 21(3): 286-289.

Eshraghi A, Kim J, Walls AC, Ledvina HE, Miller CN, Ramsey KM, Whitney JC, Radey MC, Peterson SB, Ruhland BR, Tran BQ, Goo YA, Goodlett DR, Dove SL, Celli J, Veesler D, Mougous JD (2016) Secreted effectors encoded within and outside of the Francisella pathogenicity island promote intramacrophage growth. Cell Host Microbe 20(5): 573-83.

Wexler AG, Bao Y, Whitney JC, BoBay LM, Xavier JB, Schofield WB, Barry NA, Russell AB, Goo YA, Goodlett DR, Ochman H, Mougous JD, Goodman AL (2016) Human symbionts inject and neutralize antibacterial toxins to persist in the gut. PNAS 113(13): 3639-44.

Whitney JC, Quentin D, Sawai S, LeRoux M, Harding BN, Ledvina HE, Tran BQ, Robinson H, Goo YA, Goodlett DR, Rausner S, Mougous JD (2015) An interbacterial NAD(P)+ glycohydrolase toxin requires elongation factor TU for delivery to target cells. Cell 163(3): 607-19.

LeRoux M, Peterson SB, Mougous JD (2015) Bacterial danger sensing. Journal of Molecular Biology 427(23): 3744-53. 

McFarland KA, Dolben EL, LeRoux M, Kambara TK, Ramsey KM, Kirkpatrick RL, Mougous JD, Hogan DA, Dove SL (2015) A self-lysis pathway that enhances the virulence of a pathogenic bacterium. PNAS 112(27):8433-8.

LeRoux M, Kirkpatrick RL, Montauti EI, Tran BQ, Peterson SB, Harding BN, Whitney JC, Russell AB, Traxler B, Goo YA, Goodlett DR, Wiggins PA, Mougous JD (2015) Kin cell lysis is a danger signal that activates antibacterial pathways of Pseudomonas aeruginosa. eLife 10.7554/eLife.05701.

Chou S, Daugherty MD, Peterson SB, Biboy J, Yang Y, Jutras BL, Fritz-Laylin LK, Ferrin MA, Harding BN, Jacobs-Wagner C, Yang XF, Vollmer W, Malik HS, Mougous JD (2015) Transferred interbacterial antagonism genes augment eukaryotic innate immune function. Nature 518(7537): 98-101.

Russell AB, Wexler AG, Harding BN, Whitney JC, Bohn AJ, Goo YA, Tran BQ, Barry NA, Zheng H, Peterson SB, Chou S, Gonen T, Goodlett DR, Goodman AL, Mougous JD (2014) A type VI secretion-related pathway in Bacteroidetes mediates interbacterial antagonism. Cell Host Microbe 16(2): 227-36.

Whitney JC, Beck CM, Goo YA, Russell AB, Harding B, De Leon JA, Cunningham DA, Tran BQ, Low DA, Goodlett DR, Hayes CS, Mougous JD (2014) Genetically distinct pathways guide effector export through the type VI secretion system. Molecular Microbiology 92(3): 529-42.

Russell AB, Peterson SB, Mougous JD (2014) Type VI secretion system effectors: poisons with a purpose. Nature Reviews Microbiology 51(5): 584-93.

Silverman JM, Agnello DM, Zheng H, Andrews BT, Li M, Catalano CE, Gonen T, Mougous JD (2013) Haemolysin coregulated protein is an exported receptor and chaperone of type VI secretion substrates. Molecular Cell 10.1016/j.molcel.2013.07.025.

Whitney JC, Chou S, Russell AB, Biboy J, Gardiner TE, Ferrin MA, Brittnacher M, Vollmer W, Mougous JD (2013) Identification, structure and function of a novel type VI secretion peptidoglycan glycoside hydrolase effector-immunity pair. Journal of Biological Chemistry 288(27): 26616-24

Russell AB, Leroux M, Hathazi K, Agnello DM, Ishikawa T, Wiggins PA, Wai SN, Mougous JD (2013) Diverse type VI secretion phospholipases are functionally plastic antibacterial effectors. Nature 496(7446): 508-512

LeRoux M, De Leon J, Kuwada NJ, Russell AB, Pinto-Santini D, Hood RD, Agnello DM, Robertson SM, Wiggins PA, Mougous JD (2012) Quantitative single-cell characterization of bacterial interactions reveals type VI secretion is a double-edged sword. PNAS 109(48): 19804-9.

Silverman JM, Brunet YR, Cascales E, Mougous JD (2012) Structure and regulation of the type VI secretion system. Annual Review of Microbiology 66: 453-72.

Casabona MG, Silverman JM, Sall KM, Boyer F, Couté Y, Poirel J, Grunwald D, Mougous JD, Elsen S, Attree I (2012) An ABC transporter and an outer membrane lipoprotein participate in posttranslational activation of type VI secretion in Pseudomonas aeruginosa. Environmental Microbiology 15(2): 471-86.

Chou S, Bui NK, Russell AB, Lexa KW, Gardiner TE, LeRoux M, Vollmer W, Mougous JD (2012) Structure of a peptidoglycan amidase effector targeted to gram-negative bacteria by the type VI secretion system. Cell Reports 1(6): 656-64.

Russell AB, Singh P, Brittnacher M, Bui NK, Hood RD, Carl MA, Agnello DM, Schwarz S, Goodlett DR, Vollmer W, Mougous JD (2012) A widespread bacterial type VI secretion effector superfamily identified using a heuristic approach. Cell Host and Microbe 11(5): 539-549. 

Mo Li, Le Trong I, Carl MA, Larson ET, Chou S, De Leon J, Dove SL, Stenkamp RE, Mougous JD  (2012) Structural basis for type VI secretion effector recognition by a cognate immunity protein. PLoS Pathogens 8(4): e1002613.

Silverman JM, Austin LS, Hsu F, Hicks KG, Hood RD, Mougous JD  (2011) Separate inputs modulate phosphorylation-dependent and -independent type VI secretion activation. Molecular Microbiology 82(5): 1277-90.

Russell AB, Hood RD, Bui NK, LeRoux M, Vollmer W, Mougous JD (2011) Type VI secretion delivers bacteriolytic effectors to target cells. Nature 475(7356): 343-7.

Schwarz S, Hood RD, Mougous JD (2010) What is type VI secretion doing in all those bugs? Trends in Microbiology 18(12): 531-7.

Schwarz S, West TE, Boyer F, Chiang WC, Carl MA, Hood RD, Rohmer L, Tolker-Nielsen T, Skerrett SJ, Mougous JD (2010) Burkholderia type VI secretion systems have distinct roles in eukaryotic and bacterial cell interactions. PLoS Pathogens 6(8): e1001068.

Hood RD, Singh P, Hsu F, Güvener T, Carl MA, Trinidad RR, Silverman JM, Ohlson BB, Hicks KG, Plemel RL, Li M, Schwarz S, Wang WY, Merz AJ, Goodlett DR, Mougous JD (2010) A type VI secretion system of Pseudomonas aeruginosa targets a toxin to bacteria. Cell Host and Microbe 7(1): 25-37.

Wollack JW, Silverman JM, Petzold CJ, Mougous JD, Distefano MD (2009) A minimalist substrate for enzymatic peptide and protein conjugation. ChemBioChem 10(18): 2934-43.

Hsu F, Schwarz S, Mougous JD (2009) TagR promotes PpkA-catalysed type VI secretion activation in Pseudomonas aeruginosa. Molecular Microbiology 72(5): 1111-25.

Ballister ER, Lai AH, Zuckermann RN, Cheng Y, Mougous JD (2008) In vitro self-assembly of tailorable nanotubes from a simple protein building block. PNAS 105(10): 3733-8.


Mougous JD, Gifford CA, Ramsdell TL, Mekalanos JJ
(2007) Threonine phosphorylation post-translationally regulates Pprotein secretion in Pseudomonas. Nature Cell Biology 9(7): 797-803.

Senaratne RH, Mougous JD, Reader JR, Williams SJ, Zhang T, Bertozzi CR, Riley LW (2007) Vaccine efficacy of an attenuated but persistent Mycobacterium tuberculosis cysH mutant. Journal of Medical Microbiology 56(Pt 4): 454-8. 

Jain M, Petzold CJ, Schelle MW, Leavell MD, Mougous JD, Bertozzi CR, Leary JA, Cox JS (2007) Lipidomics reveals control of Mycobacterium tuberculosis virulence lipids via metabolic coupling. PNAS 104(12): 5133-5138. 

Mougous JD, Cuff ME, Raunser S, Shen A, Zhou M, Gifford CA, Goodman AL, Joachimiak G, Ordonez CL, Lory S, Walz T, Joachimiak A, Mekalanos JJ (2006) A virulence locus of Pseudomonas aeruginosa encodes a protein secretion apparatus. Science 312(5779): 1526-1530. 

Mougous JD, Lee DH, Hubbard SC, Schelle MW, Leavell MD, Cox JS, Leary JA, Riley LW, Bertozzi CR (2006) A sulfated metabolite produced by stf3 negatively regulates the virulence of Mycobacterium tuberculosis. PNAS 103(11): 4258-63.  

Seraratne RH, De Silva AD, Williams SJ, Mougous JD, Reader JR, Zhang T, Chan S, Sidders B, Lee DH, Chan J, Bertozzi CR, Riley LW (2006) 5’-Adenosinephosphosulphate reductase (CysH) protects Mycobacterium tuberculosis against free radicals during chronic infection phase in mice. Molecular Microbiology 59(6): 1744-53. 

Mougous JD, Lee DJ, Hubbard SC, Schelle MW, Vocadlo DJ, Berger JM, Bertozzi CR (2006) Molecular basis for G protein control of prokaryotic ATP sulfurylase. Molecular Cell 21(1): 109-22. 

Pi N, Hoang MB, Gao H, Mougous JD, Bertozzi CR, Leary JA (2005) Kinetic measurements and mechanism determination of Stf0 sulfotransferase using mass spectrometry. Analytical Biochemistry 341(1): 94-104. 

Mougous JD, Petzoid CJ, Senaratne RH, Lee DH, Akey DL, Lin FL, Munchel SE, Pratt MR, Riley LW, Leary JA, Berger JM, Bertozzi CR (2004) Identification, function and structure of the mycobacterial sulfotransferase that initiates sulfolipid-1 biosynthesis. Nature Structure & Molecular Biology 11(8): 721-9. 

Woodruff PJ, Carlson BL, Siridechadilok B, Pratt MR, Senaratne RH, Mougous JD, Filey LW, Williams SJ, Bertozzi CR (2004) Trehalose is required for growth of Mycobacterium smegmatis. The Journal of Biological Chemistry 279(28): 28835-43. 

Pi N, Yu Y, Mougous JD, Leary JA (2004) Observation of a hybrid random ping-pong mechanism of catalysis for NodST: a mass spectrometry approach. Protein Science 13(4): 903-12. 

Converse SE, Mougous JD, Leavell MD, Leary JA, Bertozzi CR, Cox JS (2003) Mmpl8 is required for sulfolipid-1 biosynthesis and Mycobacterium tuberculosis virulence. PNAS 100(10): 6121-6. 

Mougous JD, Leavell MD, Senaratne RH, Leigh CD, Williams SJ, Riley LW, Leary JA, Bertozzi CR (2002) Discovery of sulfated metabolites in mycobacteria with a genetic and mass spectrometric approach. PNAS 99(26): 17037-42.

Williams SJ, Senaratne RH, Mougous JD, Riley LW, Bertozzi CR (2002) 5’-adenosinephosphosulfate lies at a metabolic branch point in mycobacteria. The Journal of Biological Chemistry 277(36): 767-76. 

Mougous JD, Green RE, Williams SJ, Brenner SE, Bertozzi CR (2002) Sulfotransferases and sulfatases in mycobacteria. Chemistry & Biology 9(7) 767-76.

Mougous JD, Brackley AJ, Foland K, Baker RT, Patrick DL (2000) Formation of uniaxial molecular films by liquid-crystal imprinting in a magnetic field. Phys. Rev. Lett. 84(12): 2742-5