Mougous Lab

University of Washington

Publications

MOUGOUS LAB

Whitney JC, Beck CM, Goo YA, Russell AB, Harding B, De Leon JA, Cunningham DA, Tran BQ, Low DA, Goodlett DR, Hayes CS, Mougous JD (2014) Genetically distinct pathways guide effector export through the type VI secretion system. Molecular Microbiology 10.1111/mmi.12571.

Russell AB, Peterson SB, Mougous JD (2014) Type VI secretion system effectors: poisons with a purpose. Nature Reviews Microbiology 10.1038/nrmicro3185.

Silverman JM, Agnello DM, Zheng H, Andrews BT, Li M, Catalano CE, Gonen T, Mougous JD (2013) Haemolysin Coregulated Protein Is an Exported Receptor and Chaperone of Type VI Secretion Substrates. Molecular Cell 10.1016/j.molcel.2013.07.025.

Whitney JC, Chou S, Russell AB, Biboy J, Gardiner TE, Ferrin MA, Brittnacher M, Vollmer W, Mougous JD (2013) Identification, structure and function of a novel type VI secretion peptidoglycan glycoside hydrolase effector-immunity pair. Journal of Biological Chemistry 10.1074/jbc.M113.488320.

Russell AB, Leroux M, Hathazi K, Agnello DM, Ishikawa T, Wiggins PA, Wai SN, Mougous JD (2013) Diverse type VI secretion phospholipases are functionally plastic antibacterial effectors. Nature 10.1038/nature12074.

LeRoux M, De Leon J, Kuwada NJ, Russell AB, Pinto-Santini D, Hood RD, Agnello DM, Robertson SM, Wiggins PA, Mougous JD (2012) Quantitative single-cell characterization of bacterial interactions reveals type VI secretion is a double-edged sword. PNAS 10.1073/pnas.1213963109.

Silverman JM, Brunet YR, Cascales E, Mougous JD (2012) Structure and regulation of the type VI secretion system. Annual Review of Microbiology 66:453-72.

Casabona MG, Silverman JM, Sall KM, Boyer F, Couté Y, Poirel J, Grunwald D, Mougous JD, Elsen S, Attree I (2012) An ABC transporter and an outer membrane lipoprotein participate in posttranslational activation of type VI secretion in Pseudomonas aeruginosa. Environmental Microbiology 10.1111/j.1462-2920.2012.02816.x.

Chou S, Bui NK, Russell AB, Lexa KW, Gardiner TE, LeRoux M, Vollmer W, Mougous JD (2012) Structure of a peptidoglycan amidase effector targeted to gram-negative bacteria by the type VI secretion system. Cell Reports 10.1016/j.celrep.2012.05.016

Russell AB, Singh P, Brittnacher M, Bui NK, Hood RD, Carl MA, Agnello DM, Schwarz S, Goodlett DR, Vollmer W, Mougous JD (2012) A widespread bacterial type VI secretion effector superfamily identified using a heurisitic approach. Cell Host and Microbe 11(5): 539-549. 

Mo Li, Le Trong I, Carl MA, Larson ET, Chou S, De Leon J, Dove SL, Stenkamp RE, Mougous JD  (2012) Structural basis for type VI secretion effector recognition by a cognate immunity protein. PLoS Pathogens 8(4): e1002613.

Silverman JM, Austin LS, Hsu F, Hicks KG, Hood RD, Mougous JD  (2011) Separate inputs modulate phosphorylation-dependent and -independent type VI secretion activation. Molecular Microbiology 82(5): 1277-90.

Russell AB, Hood RD, Bui NK, LeRoux M, Vollmer W, Mougous JD (2011) Type VI secretion delivers bacteriolytic effectors to target cells. Nature 475(7356): 343-7.

Schwarz S, Hood RD, Mougous JD (2010) What is type VI secretion doing in all those bugs? Trends in Microbiology 18(12): 531-7.

Schwarz S, West TE, Boyer F, Chiang WC, Carl MA, Hood RD, Rohmer L, Tolker-Nielsen T, Skerrett SJ, Mougous JD (2010) Burkholderia type VI secretion systems have distinct roles in eukaryotic and bacterial cell interactions. PLoS Pathogens 6(8): e1001068.

Hood RD, Singh P, Hsu F, Güvener T, Carl MA, Trinidad RR, Silverman JM, Ohlson BB, Hicks KG, Plemel RL, Li M, Schwarz S, Wang WY, Merz AJ, Goodlett DR, Mougous JD (2010) A type VI secretion system of Pseudomonas aeruginosa targets a toxin to bacteria. Cell Host and Microbe 7(1): 25-37.

Wollack JW, Silverman JM, Petzold CJ, Mougous JD, Distefano MD (2009) A minimalist substrate for enzymatic peptide and protein conjugation. ChemBioChem 10(18): 2934-43.

Hsu F, Schwarz S, Mougous JD (2009) TagR promotes PpkA-catalysed type VI secretion activation in Pseudomonas aeruginosa. Molecular Microbiology 72(5): 1111-25.

Ballister ER, Lai AH, Zuckermann RN, Cheng Y, Mougous JD (2008) In vitro self-assembly of tailorable nanotubes from a simple protein building block. PNAS 105(10): 3733-8.

PRIOR PUBLICATIONS

Mougous JD, Gifford CA, Ramsdell TL, Mekalanos JJ
(2007) Threonine phosphorylation post-translationally regulates Pprotein secretion in Pseudomonas. Nature Cell Biology 9(7): 797-803.

Senaratne RH, Mougous JD, Reader JR, Williams SJ, Zhang T, Bertozzi CR, Riley LW (2007) Vaccine efficacy of an attenuated but persistent Mycobacterium tuberculosis cysH mutant. Journal of Medical Microbiology 56(Pt 4): 454-8. 

Jain M, Petzold CJ, Schelle MW, Leavell MD, Mougous JD, Bertozzi CR, Leary JA, Cox JS (2007) Lipidomics reveals control of Mycobacterium tuberculosis virulence lipids via metabolic coupling. PNAS 104(12): 5133-5138. 

Mougous JD, Cuff ME, Raunser S, Shen A, Zhou M, Gifford CA, Goodman AL, Joachimiak G, Ordonez CL, Lory S, Walz T, Joachimiak A, Mekalanos JJ (2006) A virulence locus of Pseudomonas aeruginosa encodes a protein secretion apparatus. Science 312(5779): 1526-1530. 

Mougous JD, Lee DH, Hubbard SC, Schelle MW, Leavell MD, Cox JS, Leary JA, Riley LW, Bertozzi CR (2006) A sulfated metabolite produced by stf3 negatively regulates the virulence of Mycobacterium tuberculosis. PNAS 103(11): 4258-63.  

Seraratne RH, De Silva AD, Williams SJ, Mougous JD, Reader JR, Zhang T, Chan S, Sidders B, Lee DH, Chan J, Bertozzi CR, Riley LW (2006) 5’-Adenosinephosphosulphate reductase (CysH) protects Mycobacterium tuberculosis against free radicals during chronic infection phase in mice. Molecular Microbiology 59(6): 1744-53. 

Mougous JD, Lee DJ, Hubbard SC, Schelle MW, Vocadlo DJ, Berger JM, Bertozzi CR (2006) Molecular basis for G protein control of prokaryotic ATP sulfurylase. Molecular Cell 21(1): 109-22. 

Pi N, Hoang MB, Gao H, Mougous JD, Bertozzi CR, Leary JA (2005) Kinetic measurements and mechanism determination of Stf0 sulfotransferase using mass spectrometry. Analytical Biochemistry 341(1): 94-104. 

Mougous JD, Petzoid CJ, Senaratne RH, Lee DH, Akey DL, Lin FL, Munchel SE, Pratt MR, Riley LW, Leary JA, Berger JM, Bertozzi CR (2004) Identification, function and structure of the mycobacterial sulfotransferase that initiates sulfolipid-1 biosynthesis. Nature Structure & Molecular Biology 11(8): 721-9. 

Woodruff PJ, Carlson BL, Siridechadilok B, Pratt MR, Senaratne RH, Mougous JD, Filey LW, Williams SJ, Bertozzi CR (2004) Trehalose is required for growth of Mycobacterium smegmatis. The Journal of Biological Chemistry 279(28): 28835-43. 

Pi N, Yu Y, Mougous JD, Leary JA (2004) Observation of a hybrid random ping-pong mechanism of catalysis for NodST: a mass spectrometry approach. Protein Science 13(4): 903-12. 

Converse SE, Mougous JD, Leavell MD, Leary JA, Bertozzi CR, Cox JS (2003) Mmpl8 is required for sulfolipid-1 biosynthesis and Mycobacterium tuberculosis virulence. PNAS 100(10): 6121-6. 

Mougous JD, Leavell MD, Senaratne RH, Leigh CD, Williams SJ, Riley LW, Leary JA, Bertozzi CR (2002) Discovery of sulfated metabolites in mycobacteria with a genetic and mass spectrometric approach. PNAS 99(26): 17037-42.

Williams SJ, Senaratne RH, Mougous JD, Riley LW, Bertozzi CR (2002) 5’-adenosinephosphosulfate lies at a metabolic branch point in mycobacteria. The Journal of Biological Chemistry 277(36): 767-76. 

Mougous JD, Green RE, Williams SJ, Brenner SE, Bertozzi CR (2002) Sulfotransferases and sulfatases in mycobacteria. Chemistry & Biology 9(7) 767-76.

Mougous JD, Brackley AJ, Foland K, Baker RT, Patrick DL (2000) Formation of uniaxial molecular films by liquid-crystal imprinting in a magnetic field. Phys. Rev. Lett. 84(12): 2742-5