• Merz Lab Home

Current Procedures

(Not all protocols listed are online yet. Or ever, probably. Patience.)

Media, Glassware, etc.

• Washing glass and plasticware
• Washing and Care of Replica Velvets
• Media Recipes
• Buffer Recipes

Genetics

• The Ten Commandments of using Microbial Strains [strain_commandments.pdf]
• Yeast Genetic Nomenclature: at SGD [PDF file] and at Fred Sherman's Yeast Site [HTML].

Protein Purification

• FPLC Fittings Primer: how to run Pharmacia columns on Bio-Rad or other chromatography systems (Merz) [lcfittings.pdf]

Fusion Methods

• Yeast Vacuole Prep (Merz/Schwartz, Dec. 2005) [vacprep1.pdf]
• Homotypic Vacuole Fusion Assay
• Fusion Assay Buffers & Reagents
• The King James Protocol - Comprehensive vacuole isolation and fusion protocol, kindly provided & updated by Fusion Masters Nathan Margolis and Bill Wickner [kj_fusion_6.pdf]
• Merz and Wickner (2005) - Kinetics of Docking and Fusion paper [PDF]
• In vitro assay of calcium efflux from vacuoles [ca_assay.pdf]. Per Merz and Wickner 2004, JCB [PDF]

Immunology

• Antibody-affinity column construction (AffiGel-10 resin) and affinity-purification of antibodies
• Antibody-affinity column construction (SulfoLink resin) and affinity-purification of antibodies
• Serum Manifest Form
• Affinity Support Manifest Form
• Antibody Manifest Form
• Primary Antibody Working Stock Manifest Form (Merz, Jan. 2006) [pas_manifest.pdf]

Microscopy

• Microscope filter transmission curves
• Cleaning coverslips

Archived Procedures

Old versions of current procedures will be arcived here.

Links

Yeast

• Intro to Genetics & Molbio of Yeast
• WWW Virtual Library: Yeast
• Boone Lab: Yeast Protocols
• Gietz Lab: Yeast DNA transformation
• Gottschling Lab: Yeast Protocols
• Cora Styles: Yeast Idiosyncracies