As cargos move through the endocytic pathway the organelles within which they reside undergo a maturation process. The lumen acidifies, phosphoinositide lipids on the organelle membrane are remodeled, and proteins that mark the early endosome are selectively removed while proteins characteristic of later ensodomes and lysosomes are added to the organelle through fusion events. At the same time a remarkable morphological transition is sequestering cargos into intraluminal vesicles (ILVs) to form the multivesicular body (MVB).
A key transition in this elaborate dance occurs when the late endosome ceases to accept inbound traffic from the plasma membrane and Golgi (processes dependent on small G proteins of the Rab5 family), and becomes competent to fuse with the terminal lysosomal compartment (an event controlled by Rab7). The transition from Rab5 to Rab7 signaling is clearly related to the formation of the MVB morphology, but the specific signals that trigger the Rab5-to-Rab7 transition are poorly understood.
Recently, we have identified key enzymes and other regulators that control the signaling state of Rab5 and Rab7. At the same time our collaborators in the Odorizzi group at the University of Colorado, Boulder have made substantial process in linking molecules that drive MVB formation to the Rab5-Rab7 transition. Going forward we will use genetic and cell biological approaches, electron microscope (EM) tomography, and biochemical reconstitution to build and test models for this critical event in organelle maturation.
Shideler T, Nickerson DP, Merz AJ, Odorizzi G. 2015. Ubiquitin binding by the CUE domain promotes endosomal localization of the Rab5 GEF Vps9. Molecular Biology of the Cell — doi 10.1091/mbc.E14-06-1156.
Angers C and Merz AJ. 2011. Review: New links between vesicle coats and Rab-mediated vesicle targeting. Seminars in Cell & Developmental Biology 22:18-26.25. —pdf
Paulsel AL, Merz AJ, Nickerson DP. 2013. Vps9 family protein Muk1 is a second Rab5 GEF in budding yeast. Journal of Biological Chemistry 288:18162-71. —pdf.
Nickerson DP, *Russell MRG, *Lo SY Milnes J and Merz AJ. 2012. Termination of isoform-specific Vps21/Rab5 signaling at endolysosomal organelles by Msb3/Gyp3. Traffic 13:1411-28. *Equal contributors. —pdf
*Plemel RL, *Lobingier BT, Brett CL, Nickerson, DP, Angers CG, Paulsel A, Sprague D and Merz AJ. 2011. Subunit organization and Rab interactions of Vps-C complexes that control endolysosomal membrane traffic. Molecular Biology of the Cell 22:1353-63. *Equal contributors. —pdf
Brett CL, Plemel RL, Lobingier BT, Vignali M, Fields S and Merz AJ. 2008. Efficient termination of vacuolar Rab GTPase signaling requires coordinated action by a GAP and a protein kinase. Journal of Cell Biology 182:1141-51. *JCB featured paper; Faculty of 1000 Recommended. —pdf