INSTRUCTIONS: In the first column, enter the ratios of lipids you would like to have in your final mixture. For instance, if you would like a 1:1:2 molar ratio mixture of three lipids, enter 1.0, 1.0, and 2.0 in the first column in three different rows, and set the other rows to 0.0. Then, set the concentrations of your stock solutions in mg/mL. Select the lipid from the drop down menu.
Note that fluorescent lipids are entered only as a mole percentage of the final mixture!
If you'd like a different final volume than 35 microliters, enter it in the box labeled "uL final volume". If you like, you can adjust the molar ratios to mole percents by clicking the "Adjust Ratios" button. Otherwise, click on "Calculate Ratios." The boxes at the far right will now indicate what volume of your stock solution to mix together to achieve the desired ratio. Additionally, you will see displayed the total mass of lipid which you are mixing together.
For your convenience, the molar mass of each lipid is listed in parenthesis next to its name in the drop down menu. If you like, you can approximate one lipid with another of similar mass.