Bruker/Skyscan 1272 Availability Calendar and Scheduling
This instrument is located at Seattle Children's Research Institute, Jack MacDonald Building.
Address: 1900 Ninth Ave, Seattle WA 98101
Useful Docs
Skyscan 1272 CMOS edition info at Bruker
New Investigator Intake Form
Usage Terms & Fee Structure
Scanner Usage Instructions and Literature
Supply List
Sample Datasets
You can see more details about acquisition parameters in the log file provided with the zip archive. To visualize the data you can use open-source
3D Slicer biomedical visualization platform, and install its SlicerMorph extension.
See the tutorial for SkyScanReconImport module of the SlicerMorph on how to load these datasets into Slicer with one click.
Adult Mouse Skull (dry): 1K camera, 24 micron, scan duration 40 min. Size ~0.5GB
Adult Mouse Skull (dry): 4K camera, 6.125 micron, scan duration 8.5h. Size ~35GB
Iodine stained, adult mouse kidney: 1K camera, 18 micron, scan duration 40h. Size ~0.5GB
Iodine stained, E15 whole mouse embryo: 2K, 9 micron, scan duration 1h 50m. Size ~5GB
Full body adult mouse scan: 512 camera mode, 52.3 micron, scan duration 53 m. Size ~0.6GB
3D Visualizations
All visualizations are done using 3D Slicer and SlicerMorph.
Adult Mouse Brain perfused with vascular dye
Adult Mouse Heart perfused with vascular dye
Iodine stained E15 mouse fetus and its segmentations
Sample preparation techniques
Wong et al. 2012. Structural Stabilization of Tissue for Embryo Phenotyping Using Micro-CT with Iodine Staining
Metscher 2011. X-ray Microtomographic Imaging of Intact Vertebrate Embryos (paywalled)
Bruker's technical note on soft tissue imaging of zebrafish
Bruker's technical note on increasing contrast in EtOH or Formalin preserved specimens
Angiofil: a novel radio-contrast agent for post-mortem micro-angiograph
Funding Acknowledgement
The instrument is made available by a NIH/OD S10 shared instrument grant (S10OD032302) to Seattle Children's Research Institute.
Availability Calendar:
Making Reservations
Please contact Murat Maga if you have questions.