Cryo-electron microscope and auxiliary equipment


Cryo-electron microscope and auxiliary equipment

Cryo-electron microscope

TF20 in MolES G44L [Where is it?]

The new TF20 TEM is housed in the new Molecular Engineering and Science building (G44L). It fulfills the basic needs of a general research environment (with high-tilt applications such as diffraction experiments) or perhaps a cross-disciplinary laboratory such as a joint Materials Science/Life Science establishment.

It is suitable for both cryoelectron microscopy and cryoelectron tomography. Its features include:

Auxiliary equipment

Cryo-holder in MolES G44L

The Gatan 626 single tilt liquid nitrogen cryo transfer holder is designed for low temperature transfer and subsequent recording of radiation-sensitive, frozen-hydrated specimens for cryoEM and cryoET.

Transfer station in MolES G44L

The cryo workstation provides a protected environment for loading the frozen-hydrated specimen. A one-piece cryo shield encapsulates the frozen-hydrated grid protecting it from contamination during atmospheric transfers from the cryo workstation to the electron microscope.

Pumping station in MolES G44L

The Gatan 655 Dry Pumping Station is a completely self-contained, compact, light-weight, benchtop pumping station. It incorporates the latest diaphragm pump and molecular drag pump technologies to produce a clean, dry vacuum in the low 10-6 Torr range.

Carbon coater in HSB G524 [Where is it?]

The 208C High Vacuum Turbo Carbon Coater offers high quality carbon coating techniques for TEM, STEM, SEM, EDS/WDS, EBSD and microprobe applications.

Main Features of the 208C include:

PELCO easiGlow Glow Discharge system in HSB G524

Applying a potential difference (of a few 100 V to a few kV) between two electrodes. Electrons that are emitted from the cathode are accelerated away from the cathode, and give rise to collisions with the gas atoms or molecules in a chamber (a few mTorr to 1atm). The collisions give rise to excited species, which can decay to lower levels by the emission of light. This process makes that a gas discharge plasma typically emits a characteristic glow (and is therefore also called “glow” discharge). 

Last updated: 4/13/2013