Useful documents and links


Useful documents

  • TEM operating instructions

  • TEM note page

  • Negative staining protocol

  • cryo-sample preparation using vitrobot

  • TEM basics


    Useful links

  • TEM information without too much math.
  • TEM information with simulations.
  • Chimera is one of the best visualization software.

  • EMAN is a software to reconstruct 3D structure based on EM images. The contact is Steve Ludtke at Baylor College of Medicine.
  • XMIPP is a software to reconstruct 3D structure based on EM images. It uses maximum-likelihood classification.
  • Relion is a stand-alone computer program that employs an empirical Bayesian approach to refinement of (multiple) 3D reconstructions or 2D class averages in electron cryo-microscopy (cryo-EM). It uses maximum-likelihood classification.
  • Spider is a software to reconstruct 3D structure based on EM images. It is developed and maintained at Wadsworth center in NY.
  • Imagic is a software to reconstruct 3D structure based on EM images.
  • Frealign is a software to reconstruct 3D structure based on EM images. The contact is Nikolaus Grigorieff at Brandeis University.
  • Sparx is a software to reconstruct 3D structure based on EM images. It relies on the EMAN2 library. The contact is Pawel Penczek in the Department of Biochemistry and Molecular Biology at the University of Texas - Houston Medical School.