This cryoEM belongs to the Department of Biological Structure, and is dedicated to cryo-samples and biological samples.
To access this cryoEM, please contact Dr. Liguo Wang with your information including UW netID, a brief description of the research you plan to conduct, and the estimate of your EM usage.
To recover the cost of Service Contract and personnel, an hourly fee will be charged for the TEM usage and the TEM training. The rate is the same as the NTUF user rate. Currently, it is $70 per hour for the equipment and $70 per hour for Staff assistance including training.
The new TF20 TEM is housed in the new Molecular Engineering and Science building (G44L). It fulfills the basic needs of a general research environment (with high-tilt applications such as diffraction experiments) or perhaps a cross-disciplinary laboratory such as a joint Materials Science/Life Science establishment.
It is suitable for both cryoelectron microscopy and cryoelectron tomography. Its features include:
The Gatan 626 single tilt liquid nitrogen cryo transfer holder is designed for low temperature transfer and subsequent recording of radiation-sensitive, frozen-hydrated specimens for cryoEM and cryoET.
The cryo workstation provides a protected environment for loading the frozen-hydrated specimen. A one-piece cryo shield encapsulates the frozen-hydrated grid protecting it from contamination during atmospheric transfers from the cryo workstation to the electron microscope.
The Gatan 655 Dry Pumping Station is a completely self-contained, compact, light-weight, benchtop pumping station. It incorporates the latest diaphragm pump and molecular drag pump technologies to produce a clean, dry vacuum in the low 10-6 Torr range.
The 208C High Vacuum Turbo Carbon Coater offers high quality carbon coating techniques for TEM, STEM, SEM, EDS/WDS, EBSD and microprobe applications.
Main Features of the 208C include:
Applying a potential difference (of a few 100 V to a few kV) between two electrodes. Electrons that are emitted from the cathode are accelerated away from the cathode, and give rise to collisions with the gas atoms or molecules in a chamber (a few mTorr to 1atm). The collisions give rise to excited species, which can decay to lower levels by the emission of light. This process makes that a gas discharge plasma typically emits a characteristic glow (and is therefore also called “glow” discharge).
Last updated: 4/13/2013