Cryo-electron microscope and auxiliary equipment

• Samples to be observed by this cryoEM

  This cryoEM belongs to the Department of Biological Structure, and is dedicated to cryo-samples and biological samples.
  

• Access to this cryoEM

  To access this cryoEM, please contact Dr. Liguo Wang with your information including UW netID, a brief description of the research you plan to conduct, and the estimate of your EM usage.
  

• Rate to use this cryoEM

  To recover the cost of Service Contract and personnel, an hourly fee will be charged for the TEM usage and the TEM training. The rate is the same as the NTUF user rate. Currently, it is $70 per hour for the equipment and $70 per hour for Staff assistance including training.

Cryo-electron microscope

TF20 in MolES G44L [Where is it?]

The new TF20 TEM is housed in the new Molecular Engineering and Science building (G44L). It fulfills the basic needs of a general research environment (with high-tilt applications such as diffraction experiments) or perhaps a cross-disciplinary laboratory such as a joint Materials Science/Life Science establishment.

It is suitable for both cryoelectron microscopy and cryoelectron tomography. Its features include:

• 200kV
• Field emission gun (FEG)
• Liquid nitrogen stage
• Suitable for both cryoelectron microscopy and cryoelectron tomography

Auxiliary equipment

Cryo-holder in MolES G44L

The Gatan 626 single tilt liquid nitrogen cryo transfer holder is designed for low temperature transfer and subsequent recording of radiation-sensitive, frozen-hydrated specimens for cryoEM and cryoET.

Transfer station in MolES G44L

The cryo workstation provides a protected environment for loading the frozen-hydrated specimen. A one-piece cryo shield encapsulates the frozen-hydrated grid protecting it from contamination during atmospheric transfers from the cryo workstation to the electron microscope.

Pumping station in MolES G44L

The Gatan 655 Dry Pumping Station is a completely self-contained, compact, light-weight, benchtop pumping station. It incorporates the latest diaphragm pump and molecular drag pump technologies to produce a clean, dry vacuum in the low 10-6 Torr range.

Carbon coater in HSB G524 [Where is it?]

The 208C High Vacuum Turbo Carbon Coater offers high quality carbon coating techniques for TEM, STEM, SEM, EDS/WDS, EBSD and microprobe applications.

Main Features of the 208C include:

• Voltage controlled rod source gives multiple evaporation capabilities Feedback loop enables precise and consistent coating thickness
• Choice of operation methods for optimized operation
• Automatic evaporation control gives ease of use in busy environments Low cost
• 80 ltr/sec turbo pump on a 150mm diameter chamber gives rapid pumpdown Optional Dry Scroll Pump available

PELCO easiGlow Glow Discharge system in HSB G524

Applying a potential difference (of a few 100 V to a few kV) between two electrodes. Electrons that are emitted from the cathode are accelerated away from the cathode, and give rise to collisions with the gas atoms or molecules in a chamber (a few mTorr to 1atm). The collisions give rise to excited species, which can decay to lower levels by the emission of light. This process makes that a gas discharge plasma typically emits a characteristic glow (and is therefore also called “glow” discharge).