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Email the Director :
John Leigh, Ph.D.
leighj@u.washington.edu
Publications
| Xia, Q., T. Wang, E. L. Hendrickson, T. J. Lie, M. Hackett, and J. A. Leigh. 2009. Quantitative proteomics of nutrient limitation in the hydrogenotrophic methanogen Methanococcus maripaludis. BMC Microbiol. 9:149. | Abstract |
| Hendrickson, E. L., and J. A. Leigh. 2008. Roles of coenzyme F420-reducing hydrogenases and hydrogen- and F420-dependent methylenetetrahydromethanopterin dehydrogenases in reduction of F420 and production of hydrogen during methanogenesis. J. Bacteriol. 190:4818-4821. | Abstract |
| Hendrickson, E. L., Y. Liu, G. Rosas-Sandoval, I. Porat, D. Sšll, W. B. Whitman, and J. A. Leigh. 2008. Global responses of Methanococcus maripaludis to specific nutrient limitations and growth rate. J. Bacteriol. 190:2198-2205. | Abstract |
| Dodsworth, J. A., and J. A. Leigh. 2007. NifI inhibits nitrogenase by competing with Fe protein for binding to the MoFe protein. Biochem. Biophys. Res. Commun. 364:378-382. | Abstract |
| Lie, T. J. and J. A. Leigh. 2007. Genetic screen for regulatory mutations in Methanococcus maripaludis and its use in identification of induction-deficient mutants of the euryarchaeal repressor NrpR. Appl. Environ. Microbiol. 73:6595-6600. | Abstract |
| Leigh, J. A. and J. A. Dodsworth. 2007. Nitrogen regulation in Bacteria and Archaea. Annu. Rev. Microbiol. 61:349-377. | Abstract |
| Xia, Q., E. L. Hendrickson, T. Wang, R. J. Lamont, J. A. Leigh, and M. Hackett. 2007. Protein abundance ratios for global studies of prokaryotes. Proteomics 7: 2904-2919. | Abstract |
| Hendrickson, E., A. Haydock, B. Moore, W. Whitman, and J. Leigh. 2007. Functionally distinct genes regulated by hydrogen limitation and growth rate in methanogenic Archaea. Proc. Natl. Acad. Sci. USA 104:8930-8934. | Abstract |
| Lie, T. J., J. A. Dodsworth, D. Nickle and J. A. Leigh. 2007. Diverse homologs of the archaeal repressor NrpR function similarly in nitrogen regulation. FEMS Microbiol. Lett. 271:281-288. | Abstract |
| Stolyar, S., S. Van Dien, K. L. Hillesland, N. Pinel, T. J. Lie, J. A. Leigh, and D. A. Stahl. 2007. Metabolic modeling of a mutualistic microbial community. Mol. Syst. Biol. 3:92. | Abstract |
| Hendrickson, E. L., Q. Xia, T. Wang, J. A. Leigh, and M. Hackett. 2006. Comparison of spectral counting and metabolic stable isotope labeling for use with quantitative microbial proteomics. Analyst 131:1335-1341. | Abstract |
| Dodsworth, J. A. and J. A. Leigh. 2006. Regulation of nitrogenase by 2-oxoglutarate-reversible, direct binding of a PII-like nitrogen sensor protein to dinitrogenase. Proc. Natl. Acad. Sci. USA 103:9779-9784. | Abstract |
| Xia, Q., E. L. Hendrickson, Y. Zhang, T. Wang, F. Taub, B. C. Moore, I. Porat , W. B. Whitman, M. Hackett, and J. A. Leigh 2006. Quantitative proteomics of the archaeon Methanococcus maripaludis validated by microarray analysis and real time PCR. Molecular and Cellular Proteomics 5:868-881. | Abstract |
| Porat, I., W. Kim, E. L. Hendrickson, Q. Xia, Y. Zhang, T. Wang, F. Taub, B. C. Moore, I. J. Anderson, M. Hackett, J. A. Leigh, and W. B. Whitman. 2006. Disruption of the ehb operon limits anabolic CO2 assimilation in the archaeon Methanococcus maripaludis. J. Bacteriol. 188:1373-1380. | Abstract |
| Dodsworth, J. A., N. C. Cady, and J. A. Leigh. 2005. 2-oxoglutarate and the PII homologues NifI1 and NifI2 regulate nitrogenase activity in cell extracts of Methanococcus maripaludis. Molecular Microbiol. 56:1527-1538. | Abstract |
| Moore, B.C. and J.A. Leigh. 2005. Markerless mutagenesis in Methanococcus maripaludis demonstrates roles for alanine dehydrogenase, alanine racemase, and alanine permease. J. Bacteriol. 187(3):972-9. | Abstract |
| Lie, T.J., Wood, G.E. and J. A. Leigh. 2005. Regulation of nif expression in Methanococcus maripaludis: roles of the euryarchaeal repressor NrpR, 2-oxoglutarate, and two operators. J. Biol. Chem. 280(7):5236-41 |
Abstract |
| Hendrickson, E. L., R. Kaul, Y. Zhou, D. Bovee, P. Chapman, J. Chung, E. Conway de Macario, J. A. Dodsworth, W. Gillett, D. E. Graham, M. Hackett, A. K. Haydock, A. Kang, M. L. Land, R. Levy, T. J. Lie, T. A. Major, B. C. Moore, I. Porat, A. Palmeiri, G. Rouse, C. Saenphimmachak, D. Sšll, S. Van Dien, T. Wang, W. B. Whitman, Q. Xia, Y. Zhang, F. W. Larimer, M. V. Olson, and J. A. Leigh. 2004. Complete genome sequence of the genetically tractable hydrogenotrophic methanogen Methanococcus maripaludis. J. Bacteriol. 186:6956-69 |
Abstract |
| Haydock, A. K., I. Porat, W. B. Whitman, J. A. Leigh. 2004. Continuous culture of Methanococcus maripaludis under defined nutrient conditions. FEMS Microbiol Lett. 238(1):85-91 |
Abstract |
| Wood, G. E, A. K. Haydock, and J. A. Leigh. 2003. Function and regulation of the formate dehydrogenase genes of the methanogenic archaeon Methanococcus maripaludis. J. Bacteriol. 185:2548-2554. |
Abstract |
| Lie, T. J. and J. A. Leigh. 2003. A novel repressor of nif and glnA expression in the methanogenic archaeon Methanococcus maripaludis. Molecular Microbiol. 47:235-246. |
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Lie, T. J. and J. A. Leigh. 2002. Regulatory response of Methanococcus maripaludis to alanine, an intermediate nitrogen source. J. Bacteriol. 184:5301-5306. |
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Leigh, J. A. 2002. Evolution of energy metabolism. In: Biodiversity of Microbial Life: Foundation of Earth's Biosphere, J. T. Staley and A.-L. Reysenbach, eds. | Abstract Not Available |
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Kessler, P. S., C. Daniel, and J. A. Leigh. 2001. Ammonia switch-off of nitrogen fixation in the methanogenic archaeon Methanococcus maripaludis: mechanistic features and requirement for the novel GlnB homologues, NifI1 and NifI2. J. Bacteriol. 183:882- 889. | Abstract |
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Leigh, J. A. 2000. Nitrogen fixation in methanogens - the archaeal perspective. Curr. Issues Mol. Biol. 2:125-131. |
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Kessler, P. S. and J. A. Leigh. 1999. Genetics of nitrogen regulation in Methanococcus maripaludis. Genetics 152:1343-1351. |
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Cohen-Kupiec, R., M. Kupiec, K. Sandbeck, and J. A. Leigh. 1999. Functional conservation between the argininosuccinate lyase of the archaeon Methanococcus maripaludis and the corresponding bacterial and eukaryal genes. FEMS Microbiol. Lett. 173:231-238. |
Abstract |
| Leigh, J. A. 1999. Transcriptional regulation in Archaea. Curr. Op. Microbiol. 2:131-134. | Abstract |
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Cohen-Kupiec, R., C. J. Marx, and J. A. Leigh. 1999. Function and regulation of glnA in the methanogenic archaeon Methanococcus maripaludis. J. Bacteriol. 181:256-261. | Abstract |
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Kessler, P. S., C. Blank, and J. A. Leigh. 1998. The nif gene operon of the methanogenic archaeon Methanococcus maripaludis. J. Bacteriol. 180:1504-1511. |
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Cohen-Kupiec, R., C. Blank, and J. A. Leigh. 1997. Transcriptional regulation in Archaea: In vivo demonstration of a repressor binding site in a methanogen. Proc. Natl. Acad. Sci. USA 94:1316-1320. |
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Kessler, P. S., J. McLarnan, and J. A. Leigh. 1997. Nitrogenase phylogeny and the molybdenum dependence of nitrogen fixation in Methanococcus maripaludis. J. Bacteriol. 179:541-543. |
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Argyle, J.L., Tumbula, D.L., and Leigh, J.A. 1996. Neomycin resistance as a selectable marker in Methanococcus maripaludis. Appl. Environ. Microbiol. 62:4233-4237. |
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Blank, C.E., Kessler, P.S., and Leigh, J.A. 1995. Genetics in methanogens: Transposon insertion mutagenesis of a Methanococcus maripaludis nifH gene. J. Bacteriol. 177:5773-5777. |
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Sandbeck, K.A. and Leigh, J.A. 1991. Recovery of an integration shuttle vector from tandem repeats in Methanococcus maripaludis. Appl. Environ. Microbiol. 57:2762-2763. |
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