J o h n    L e i g h    L a b

Markerless Mutagenesis Procedure

Our method for making markerless deletions in M. marialudis takes advantage of the susceptibility to purine analogs conferred by the hpt (hypoxanthine / guanine phosohoribosyltransferase) gene. We also developed a negative selection method to stably incorporate constructs into the genome, at the site of the upt gene encoding uracil phosphoribosyltransferase.

Plasmids

pCRPRTNEO plasmid map sequence text file	pBLPRT plasmid map sequence text file

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