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#
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Date
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Topic
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1
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3/30 |
Laboratory
#1.
Safety instruction. Review of sampling sites (Lake Washington).
Experiments with reverse osmosis system. Determination of
rejection and permeation at varying conditions using UV
spectrophotometry. Work with
DOC/DIC analyzer (optional).
Preparation of samples for further analyses.
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2
|
4/6 |
Laboratory #2. Spectrophotometric determination of phosphorus.
Inductively
coupled plasma analyses for metals. Ionic chromatographic
analysis for anions (optional).
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3
|
4/13 |
Laboratory #3.
Ozonation of permeate and retantates. Spectrophotometric
determination of ozone concentration. Determination
of formation of ozonation by-products (oxalate) by anionic
chromatography. Degradation of endocrine disruptor ethynyl
estradiol (EE2) by ozone.
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4
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4/20 |
Laboratory #4.
Chlorination of RO-treated solutions containing EE2.
Determination of
EE2 degradation in the presence and absence of natural organic
matter. Interpretation of the data using differential
spectroscopy and deconvolution.
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5 |
4/27 |
Laboratory #5.
Electrochemical treatment of solutions containing representative
phenolic species (phenol, EE2, nonylphenol). Colorimetric
determination of concentrations of phenols.
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6
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5/4 |
Laboratory #6.
Introductory microbiological laboratory. Standard microbiological techniques.
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7
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5/11 |
Laboratory #7
Determination of coliforms and heterotrophs in environmental
samples.
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8
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5/18 |
Laboratory #8.
Microbial degradation of phenol. Isolation of phenol-degrading
bacterial communities.
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9 |
5/25 |
Laboratory #9.
Molecular characterization of bacterial communities involved in
degradation of phenol. Microbial degradation of EE2.
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10
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6/1 |
Laboratory #10.
Gene amplification
for EE2 degrading bacteria.
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6/8 |
Group presentations and final exam (10:30-12:20 PM Wednesday)
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