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The
characterization and control of solid/liquid
interfaces is at the forefront of a range of topics
including regenerative medicine, biofouling, implant
technology, and medical diagnostics. My primary
research activities originate from the need to probe
conformation and orientation of biomolecules (DNA,
peptides, proteins, antibodies...) in contact to a
solid surface with molecular-level resolution and
under standard conditions in situ and in vitro. My
group has developed and applied femtosecond based
sum-frequency-generation (SFG) spectrometers and
several measuring cells including a thin layer
analysis and a spectro-electrochemical cell. These
have been used to study model systems such as
self-assembled monolayers (SAMs) with various
functional groups in air and liquid environments under
controlled temperature, solution, and potential
conditions. The information obtained by these
experiments facilitated our understanding of SFG
signals from more complex biomolecules such as DNA and
peptides. Other systems of interest are supported
lipid bilayers, membrane proteins, and
stimuli-responsive polymer coatings. Recent work from
my group has shown that SFG spectroscopy can be used
to interrogate ordering phenomena within the
extracellular matrix beneath adherent cells on
artificial substrates at early stages of cell
adhesion. The groundwork presented in these studies
opens the possibility for use of this technique in a
variety of other systems including the growth and
development of biofilms or dynamic processes occurring
during cell adhesion on functional surfaces.
Publications
Koelsch,
P.; Muglali, M. I.; Rohwerder, M.; Erbe, A. Third-order
effects in resonant sum-frequency-generation
signals at electrified metal/liquid interfaces.
J. Opt. Soc. Am. B
2013, 30, 219.
Muglali,
M.; Erbe, A.; Chen, Y.; Barth, C.; Koelsch, P.;
Rohwerder, M. Modulation
of electrochemical hydrogen evolution rate by
araliphatic thiol monolayers on gold. Electrochim. Acta
2013, 90, 17.
Pranzetti,
A.; Mieszkin, S.; Iqbal, P.; Rawson, F. J.; Callow,
M. E.; Callow, J. A.; Koelsch, P.; Preece, J. A.;
Mendes, P. M. An Electrically Reversible Switchable
Surface to Control and Study Early Bacterial
Adhesion Dynamics in Real-Time. Advanced Materials
2012,
accepted.
Pop-Georgievski,
O.; Verreault, D.; Diesner, M.- O.; Proks, V.;
Heissler, S.; Rypáček, F.; Koelsch, P.
Non-Fouling Poly(ethylene oxide) Layers
End-Tethered to Polydopamine. Langmuir 2012
, 28, 14273.
Barth,
C.; Jakubczyk, D.; Kubas, A.; Anastassacos, F.;
Brenner-Weiss, G.; Fink, K.; Schepers, U.;
Bräse, S.; Koelsch, P. Inter-kingdom
Signaling: Integration, Conformation and
Orientation of N-Acyl-L-Homoserine Lactones in
Supported Lipid Bilayers. Langmuir 2012, 28, 8456.
Howell, C.; Jeyachandran, Y.
L.; Koelsch, P.; Zharnikov, M.
Orientation and Ordering in Sequence- and
Length-Mismatched Surface-Bound DNA Hybrids. J. Phys. Chem. C
2012, 116, 11133.
Jakubczyk,
D.; Barth, C.; Kubas, A.; Anastassacos, F.; Koelsch,
P.; Fink, K.; Schepers, U.; Brenner-Weiß, G.;
Bräse, S. Deuterium
labelled N-acyl-L-homoserine lactones (AHLs) -
Inter-kingdom signalling molecules - synthesis,
structural studies, interaction with model lipid
membranes. Anal.
Bioanal. Chem. 2012, 403, 473.
Bruns,
M.; Barth, C.; Bruener, P.; Engin, S.; Grehl, T.; Howell, C.; Koelsch, P.; Mack, P.;Nagel,
P.; Trouillet, V.; Wedlich, D.; White, R. G. Structure
and Chemical Composition of Mixed Benzylguanine
and Methoxy Terminated SAMs for Immobilization of
Biomolecules. Surface and Interface Analysis 2012, 44, 909.
Diesner,
M.-O.; Welle, A.; Kazanci, M.; Kaiser, P.; Spatz,
J.; Koelsch, P. In
vitro observation of dynamic ordering processes in
the extracellular matrix of living, adherent cells.
Biointerphases
2011, 6, 171.
Staudacher,I.;
Wang, L.; Wan, X. P.; Obers, S.; Wenzel, W.;
Tristram, F.; Koschny, R.; Staudacher, K.;
Kisselbach, J.; Koelsch, P.; Schweizer, P. A.;
Katus, H. A.; Ficker, E.; Thomas, D. hERG
K(+) channel-associated cardiac effects of the
antidepressant drug desipramine. Naunyn.
Schmiedebergs Arch. Pharmacol. 2011, 383, 119.
Konietzny,
R.; Barth, C.; Harms, S.; Raetzke, K.; Koelsch, P.;
Staudt, C. Structural
investigations and swelling behavior of 6FDA
copolyimide thin films. Polym. Int. 2011, 60, 1670.
Schreiner, S.
M.; Hatch, A. L.; Shudy, D. F.; Howard, D. R.;
Howell, C.; Zhao, J. L.; Koelsch, P.; Zharnikov, M.;
Petrovykh, D. Y.; Opdahl, A. Impact
of DNA-Surface Interactions on the Stability of
DNA Hybrids. Anal. Chem.
2011, 83, 4288.
Howell, C.;
Zhao, J. L.; Koelsch, P.; Zharnikov, M. Hybridization
in ssDNA films-a multi-technique spectroscopy
study. Phys.
Chem. Chem. Phys. 2011, 13, 15512.
Howell, C.;
Hamoudi, H.; Heissler, S.; Koelsch, P.; Zharnikov,
M. Orientation
changes in surface-bound hybridized DNA undergoing
preparation for ex situ spectroscopic measurements.
Chem. Phys.
Lett. 2011,
513, 267.
Dusselberg,
D.; Verreault, D.; Koelsch, P.; Staudt, C. Synthesis
and characterization of novel, soluble
sulfur-containing copolyimides with high
refractive indices. J. Mater. Sci.
2011, 46, 4872.
Verreault,
D.; Kurz, V.; Howell, C.; Koelsch, P. Sample
cells for probing solid/liquid interfaces with
broadband sum-frequency-generation spectroscopy.
Rev. Sci.
Instrum. 2010, 81.
Tunc, I.;
Bruns, M.; Gliemann, H.; Grunze, M.; Koelsch, P. Bandgap
determination and charge separation in Ag@TiO(2)
core shell nanoparticle films. Surf. Interface
Anal. 2010,
42, 835.
Kurz, V.;
Grunze, M.; Koelsch, P. In
Situ Characterization of Thermo-Responsive
Poly(N-Isopropylacrylamide) Films with
Sum-Frequency Generation Spectroscopy. ChemPhysChem
2010, 11, 1425.
Howell, C.;
Maul, R.; Wenzel, W.; Koelsch, P. Interactions
of hydrophobic and hydrophilic self-assembled
monolayers with water as probed by
sum-frequency-generation spectroscopy. Chem. Phys.
Lett. 2010,
494, 193.
Diesner, M.
O.; Howell, C.; Kurz, V.; Verreault, D.; Koelsch, P.
In
Vitro Characterization of Surface Properties
Through Living Cells. J. Phys. Chem.
Lett. 2010,
1, 2339.
Jribi, R.;
Barthel, E.; Bluhm, H.; Grunze, M.; Koelsch, P.;
Verreault, D.; Sondergard, E. Ultraviolet
Irradiation Suppresses Adhesion on TiO(2). J. Phys. Chem.
C 2009,
113, 8273.
Fukunaga, A.;
Urayama, K.; Koelsch, P.; Takigawa, T. Electrically
driven director-rotation of swollen nematic
elastomers as revealed by polarized Fourier
transform infrared spectroscopy. Phys. Rev. E:
Stat., Nonlinear, Soft Matter Phys. 2009, 79.
Ballav, N.;
Koelsch, P.; Zharnikov, M. Orientation
and Ordering in Monomolecular Films of
Sulfur-Modified Homo-oligonucleotides on Gold.
J. Phys. Chem.
C 2009,
113,
18312.
Howell, C.;
Schmidt, R.; Kurz, V.; Koelsch, P.
Sum-frequency-generation spectroscopy of DNA films
in air and aqueous environments. Biointerphases
2008, 3, Fc47.
Howell, C.;
Diesner, M. O.; Grunze, M.; Koelsch, P. Probing
the Extracellular Matrix with
Sum-Frequency-Generation Spectroscopy. Langmuir 2008, 24, 13819.
Koelsch, P.;
Viswanath, P.; Motschmann, H.; Shapovalov, V. L.;
Brezesinski, G.; Mohwald, H.; Horinek, D.; Netz, R.
R.; Giewekemeyer, K.; Alditt, T. S.; Schollmeyer,
H.; von Klitzing, R.; Daillant, J.; Guenoun, P. Specific ion
effects in physicochemical and biological systems:
Simulations, theory and experiments. Colloids Surf.,
A 2007,
303, 110.
Aksenov, V.;
Stannarius, R.; Tammer, M.; Koelsch, P.; Kremer, F.;
Rossle, M.; Zentel, R. FTIR
spectroscopy of smectic elastomer films under
lateral strain. Liq. Cryst.
2007, 34, 87.
Slotta, U.;
Tammer, M.; Kremer, F.; Koelsch, P.; Scheibel, T. Structural
analysis of spider silk films. Supramol. Chem.
2006, 18, 465.
Petrov, M.;
Minofar, B.; Vrbka, L.; Jungwirth, P.; Koelsch, P.;
Motschmann, H. Aqueous
ionic and complementary zwitterionic soluble
surfactants: Molecular dynamics simulations and
sum frequency generation spectroscopy of the
surfaces. Langmuir 2006, 22, 2498.
Andersen, A.;
Oertegren, J.; Koelsch, P.; Wantke, D.; Motschmann,
H. Oscillating
bubble SHG on surface elastic and surface
viscoelastic systems: New insights in the dynamics
of adsorption layers. J. Phys. Chem.
B 2006,
110,
18466.
Schneider,
M.; Andersen, A.; Koelsch, P.; Motschmann, H.
Following fast adsorption processes with surface
plasmon spectroscopy: reflectivity versus mismatch
tracking. Sens. Actuators, B 2005, 104, 276.
Koelsch, P.;
Motschmann, H. Varying
the counterions at a charged interface. Langmuir 2005, 21, 3436.
Koelsch, P.;
Motschmann, H. Relating
foam lamella stability and surface dilational
rheology. Langmuir 2005, 21, 6265.
Koelsch, P.;
Motschmann, H. An
experimental route to Hofmeister. Curr. Opin.
Colloid Interface Sci. 2004, 9, 87.
Koelsch, P.;
Motschmann, H. A
method for direct determination of the prevailing
counterion distribution at a charged surface.
J. Phys. Chem.
B 2004,
108,
18659.
Wolf, J. F.;
Hillner, P. E.; Bilewicz, R.; Koelsch, P.; Rabe, J.
P. Novel
scanning near-field optical microscope
(SNOM)/scanning confocal optical microscope based
on normal force distance regulation and bent
etched fiber tips. Rev. Sci.
Instrum. 1999, 70, 2751.
de Ruijter, M.; Koelsch, P.; Voue, M.; De
Coninck, J.; Rabe, J. P. Effect
of temperature on the dynamic contact angle. Colloids
Surf., A 1998, 144, 235.
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