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pdf files of recent Gelb Group reprints from the list below.
Phospholipase Reprints
Protein Prenylation
Reprints
Anti-Parasite
Drug Design (other than protein prenylation) Reprints
Clinical Enzymology
and Quantitative Proteomics Reprints
Other Reprints
Phospholipase Reprints back to top
Wilensky, Robert L, Shi, Yi, Mohler, Emile R. III, Hamamdzic, Damir, Burgert, Mark E., Li, Jun, Postle, Anthony, Fenning, Robert S., Bollinger, James G., Hoffman, Bryan E., Pelchovitz, Daniel J., Yang, Jisheng, Mirabile, Rosanna C., Webb, Christine L., Zhang, LeFeng, Zhang, Ping, Gelb, Michael H., Walker, Max C., Zalewski, Andrew, and Macphee, Colin H., (2008) "Inhibition of lipoprotein-associated phospholipase A2 reduces complex coronary atherosclerotic plaque development," Nature Medicine, 14: on-line 9/2008. reprint.pdf
Oslund, RC, Cermak, N, Gelb, MH, (2008) "Highly specific and broadly potent inhibitors of mammalian secreted phospholipases A2," J. Med. Chem., submitted. reprint.pdf
Tribler, L, Jensen, LT, Jørgensen, K, Brünner, N, Gelb, MH, Nielsen, HJ and Jensen, SS, (2007) “Increased expression and activity of group IIA and X secretory phospholipase A2 in Peritumoral versus central colon carcinoma tissue,” Anticancer Research, 27:3179-3186. reprint.pdf
Morgan, D, Cherny, VV, Finnegan A, Bollinger J, Gelb MH, DeCoursey TE. (2007) “Sustained activation of proton cheannels and NADPH oxidase in human eosinophils and murine granulocytes requires PKC but not CPLA2 alpha activity,” J. Physiol., 579(Pt 2):327-344. reprint.pdf
Hallstrand, TS, Chi, EY, Singer, AG, Gelb, MH, Henderson, Jr., WR (2007) “Secreted phospholipase A2 group X overexpression in asthma and bronchial hyperresponsiveness,” Am. J. Respir Crit Care Med., to be published. reprint.pdf
Wichmann O, Gelb MH, Schultz, C. (2007) “Probing Phospholipase A2 with fluorescent phospholipid Substrates,” ChemBioChem, To be published. reprint.pdf
Ghomashchi, F., Bollinger, J.G. and Gelb, M. H. (2007) "Facile preparation of leukotrienes C4, D4 and E4 containing carbon-13 and nitrogen-15 for quantification of cysteinyl leukotrienes by mass spectrometry," J. Radiolabel. Compds Radiopharm. 50:729 reprint.pdf
Ghosh, M., Loper, R., Ghomashchi, F., Tucker, D.E., Bonventre, J.V., Gelb, M.H. and Christina C. Leslie. (2007) "Function, Activity, and Membrane Targeting of Cytosolic Phospholipase A2-zeta in Mouse Lung Fibroblasts," J. Biol. Chem. 282:11676 reprint.pdf
Henderson, W.R., Chi, E.Y., Bollinger, J.G., Tien, Y.T., Ye, X., Castelli, L. Rubtsov, Y.P., Singer, A.G., Chiang, G.K., Nevalainen, T., Rudensky, A.Y. and M. Gelb. (2007) "Importance of Group X-secreted Phospholipase A2 in Allergen-Induced airway inflammation and remodeling in a mouse asthma model," J. Exp. Med. April 16 204:865-877 reprint.pdf
Rouault M, Calvez CL, Boilard E, Surrel F, Singer A, Ghomashchi F, Bezzine S, Scarzello S, Bollinger J, Gelb MH, Lambeau G. (2007) " Recombinant production and properties of binding of the full set of mouse secreted phospholipases a(2) to the mouse m-type receptor,"
Biochemistry 46:1647-62. reprint.pdf
Boilard, E., Rouault, M., Surrel, F., Le Calvez, C. Bezzine, S., Singer, A., Gelb, M.H. and Gerard Lambeau. (2006) "Secreted Phospholipase A2 Inhibitors Are Also Potent Blockers of Binding to the M-Type Receptor," Biochemistry 45:13202 reprint.pdf
Karabina, S.A., Brocheriou, I., Le Naour, G., Agrapart, M., Durand, H., Gelb, M.H., Lambeau, G. and Ewa Ninio.(2006) "Atherogenic properties of LDL particles modified by human group X secreted phospholipase A2 on human endothelial cell function," The FASEB Journal 20:E2 [Published online Oct. 31] reprint.pdf
Ghosh, M., Loper, R., Gelb, M.H. and Leslie, C.C.(2006) “Identification of the Expressed Form of Human Cytosolic Phospholipase A2β (cPLA2β),” J. Biol. Chem. 281:16615. reprint.pdf
Gelb, M. H., Mounier, Carine M., Hefner, Ying, Watson, Steve P., Platelet Phospholipases A2. In Platelets in thrombotic and non-thrombotic disorders: pathophysiology, pharmacology, and therapeutics, Gresele, P. e. a., Ed. Cambridge University Press: Cambridge, 2002; pp 221-237. reprint.pdf
Ni,Z., Okeley, N. M., Smart, B.P., and Michael H. Gelb (2006)" Intracellular Actions of Group IIA Secreted Phospholipase A2 and Group IVA Cytosolic Phospholipase A2 Contribute to Arachidonic Acid Release and Prostaglandin Production in Rat Gastric Mucosal Cells and Transfected Human Embryonic Kidney Cells" J. Biol. Chem., 281: 16245-55 . reprint.pdf
Eerola, L.I., Surrel, F., Nevalainen, T.J., Gelb, M.H., Lambeau, G. and V. Jukka O. Laine (2006) "Analysis of expression of secreted phospholipases A2 in mouse tissues at protein and mRNA levels," Biochimica et Biophysica Acta [Epub ahead of print] April 10 reprint.pdf
Rouault, M., Rash, L. D., Escoubas, P., Boilard, E., Bollinger,J., Lomonte,B., Maurin, T., Guillaume,C., Canaan, S., Deregnaucourt, C., Schrevel,J., Doglio, A., Gutierrez, J. M., Lazdunski, M., Gelb, M. H., and G. Lambeau. (2006) "Neurotoxicity and Other Pharmacological Activities of the Snake Venom
Phospholipase A2 OS2: The N-Terminal Region Is More Important Than Enzymatic
Activity," Biochemistry [Epub ahead of print] Feb. 14 reprint.pdf
Smart, B.P., Oslund, R.C. and Walsh, L.A. (2006) " The First Potent Inhibitor of Mammalian Group
X Secreted Phospholipase A2: Elucidation of Sites for Enhanced Binding," J. Med. Chem. Feb. 8 ; [Epub ahead of print] reprint.pdf
Huhtinen, H.T., Gronroos, J.O., Gronroos, J.M. Uksila, J., Gelb, M.H., Nevalainen, T.J. and Laine, V.J. (2006) "Antibacterial effects of human group IIA and group XIIA phospholipase A2 against Helicobacter pylori in vitro," APMIS 114:127-30 reprint.pdf
Vandal, O.H., Gelb, M.H., Ehrt, S. and Nathan, C.F. (2006) "Cytosolic Phospholipase A2 enzymes are not required by mouse bone marrow-derived macrophages for the control of Mycobacterium tuberculosis in Vitro ," Infect. Immun. Mar. 74:1751-6. reprint.pdf
Nevalainen, T.J., Eerola, L.I., Rintala, E., Laine, V.J., Lambeau, G. and Gelb, M.H. (2005) " Time-resolved fluoroimmunoassays of the complete set of secreted phospholipases A2 in human serum," Biochim Biophys Acta. 1733:210-213 reprint.pdf
Forsell, P.K., Olsson, A.O., Andersson, E., Nallan, L. and Gelb, M.H. (2005) "Polychlorinated biphenyls induce arachidonic acid release in human platelets in a tamoxifen sensitive manner via activation of group IVA cytosolic phospholipase A(2)-alpha," Biochem Pharmacol. Nov. 12 ; [Epub ahead of print] reprint.pdf
Petan, T., Krizaj, I., Gelb, MH. and Pungercar, J. (2005) "Ammodytoxins, potent presynaptic neurotoxins, are also highly efficient phospholipase A(2) enzymes," Biochemistry, 44:12535-12545 reprint.pdf
Tucker, D.E., Stewart, A., Nallan, L., Bendale, P., Ghomashchi, F., Gelb, MH. and Leslie, CC. (2005)
"Group IVC cytosolic phospholipase A2
is farnesylated and palmitoylated in mammalian cells,"
J. Lipid Res., 46:2122-33. reprint.pdf
Kita, Y., Takahashi, T., Uozumi, N., Nallan, L.,Gelb, M.H. and Shimizu, T. (2005) "Pathway-oriented profiling of lipid mediators in macrophages," Biochem. Biophys. Res. Commun., 330:898-906. reprint.pdf
Nielsen, R.D., Che, K., Gelb, M.H. and Robinson, B.H. (2005) "A Ruler for Determining the Position of Proteins in Membranes," J. Am. Chem. Soc., 127:6430-42. reprint.pdf data supplement.pdf
Babon, A., Almunia, C., Boccaccio, C., Beaumelle, B., Gelb, M.H., Menez, A., Maillere, B., Abastado, J.P., Salcedo, M. and Gillet, D., (2005) "Cross-presentation of a CMV pp65 epitope by human dendritic cells using bee venom PLA(2) as a membrane-binding vector," FEBS Lett., 579:1658-64. reprint.pdf
Yuan, C., Tu, S., Gelb, M.H. and Tsai, M-D. (2005) "Unusual Four-Bond Secondary H/D Isotope Effect Supports a Short-Strong Hydrogen Bond between Phospholipase A2 and a Transition State Analogue Inhibitor," Biochemistry, 44:4748-54. reprint.pdf
Granata, F., Petraroli, A., Boilard, E., Bezzine, S., Bollinger, J., Del Vecchio, L., Gelb, M.H., Lambeau, G., Marone, G., and Triggiani M. (2005) "Activation of cytokine production by secreted phospholipase A2 in human lung macrophages expressing the m-type receptor," J. Immunol., 174: 464-74. reprint.pdf
Masuda, S., Murakami, M., Matsumoto, S., Eguchi, N., Urade, Y., Lambeau, G., Gelb, M.H., Ishikawa, Y., Ishii, T. and Kudo, I. (2004) "Localization of various secretory phospholipase A(2) enzymes in male reproductive organs," Biochim Biophys Acta., 1686:61-76. reprint.pdf
Ivanovski, G., Petan, T., Krizaj, I., Gelb, M.H., Gubensek, F. and Pungercar, J. (2004) "Basic amino acid residues in the beta-structure region contribute, but not critically, to presynaptic neurotoxicity of ammodytoxin A," Biochim Biophys Acta., 1702:217-25. reprint.pdf
Buhot, C., Chenal, A., Sanson, A., Pouvelle-Moratille, S., Gelb, M.H., Menez, A., Gillet, D. and Maillere, B. (2004) "Alteration of the tertiary structure of the major bee venom allergen Api m 1 by multiple mutations is concomitant with low IgE reactivity," Protein Sci. 13:2970-8. reprint.pdf
Smart, B.P., Pan, Y.H., Weeks, A.K., Bollinger, J.G., Bahnson, B.J. and Gelb, M.H. (2004) "Inhibition of the complete set of mammalian secreted phospholipases A(2) by indole analogues a structure-guided study," Bioorg Med Chem., 12:1737-49. reprint.pdf
Landreville, S., Coulombe, S., Carrier, P., Gelb, M.H., Guérin, S.L., and Salesse, C. (2004) Expression of Phospholipases A2 and C in Human Corneal Epitheliel Cells," IOVS, 45:3997-4003. reprint.pdf
Bollinger, J.G., Diraviyam, K., Ghomashchi, F., Murray, D., and Gelb, M.H. (2004) "Interfacial Binding of Bee Venom Secreted Phospholipase A2 to Membranes Occurs Predominantly by a Nonelectrostatic Mechanism," Biochemistry, 43:13293-13304. reprint.pdf
Rubin, B.B., Downey, G.P., Koh, A., Degousee, N., Ghomashchi, F., Nallan, L., Stefanski, E., Smart, B.P., Lindsay, T.F., Cherepanov, V., Vachon, E., Kelvin, D., Sadilek, M., Brown, G.E., Yaffe, M.B., Plumb, J., Grinstein, S., Glogauer, M., and Gelb, M.H. (2004) "Cytosolic phospholipase A2 alpha is necessary for platelet activating factor biosynthesis, efficient neutrophil mediated bacterial killing and the innate immune response to pulmonary infection," J. Biol. Chem., 280:7519-29. reprint.pdf
Haas, U., Podda, M., Behne, M., Gurrieri, S., Alonso, A., Fürstenberger, G., Pfeilschifter, J., Lambeau, G., Gelb, M.H., and Kaszkin, M. (2004) "Characterization and Differentiation-dependent Regulation of Secreted Phospholipases A2 in Human Keratinocytes and in Healthy and Psoriatic Human Skin," J. Invest. Dermatol., 124:204-211. reprint.pdf
Sved, P., Scott, K.F., McLeod, D., King, N.J., Singh, J., Tsatralis, T., Nikolov, B., Boulas, J., Nallan, L., Gelb, M.H., Sajinovic, M., Graham, G.G., Russell, P.J., and Dong, Q. (2004) "Oncogenic action of secreted phospholipase A2 in prostate cancer," Cancer Res., 64: 6934-40. reprint.pdf
Nielsen, R.D., Canaan, S., Gladden, J.A., Gelb, M.H., Mailer, C., and Robinson, B.H. (2004) "Comparing continuous wave progressive saturation EPR and time domain saturation recovery EPR over the entire motional range of nitroxide spin labels," J Magn Reson., 169:129-63. reprint.pdf
Leslie, C.C., Gelb, M.H. (2004) "Assaying phospholipase A2 activity," Methods Mol Biol., 284:229-42. reprint.pdf
Mounier, C.M., Ghomashchi, F., Lindsay, M.R., James, S., Singer, A.G., Parton, R.G., and Gelb, M.H. (2004) "Arachidonic acid release from mammalian cells transfected with human groups IIA and X secreted phospholipase A2 occurs predominantly during the secretory process and with the involvement of cytosolic phospholipase A2-a," J. Biol. Chem. 279:25024-25038. reprint.pdf, supplement
Okeley, N.M, Gelb, M.H. (2004) "Membrane Transport, Structure, Function, and Biogenesis: A designed probe for acidic phospholipids reveals the enriched anionic character of the cytosolic face of the mammalian plasma membrane," J. Biol. Chem, 279:21833-21840. reprint.pdf
Stephane Canaan, S., Zoltan Zadori, Z., Ghomashchi,F., Bollinger, J., Sadilek, M., Marie Eve Moreau, M.E., Tijssen, P., and Gelb, M.H. (2004) "Interfacial enzymology of parvovirus phospholipases A2," J. Biol. Chem, 279:14502-14508. reprint.pdf
Triggiani, M., Giannattasio, G., Balestrieri, B., Granata, F., Gelb, M.H., de Paulis, A., and Marone, G. (2004) "Differential modulation of mediator release from human basophils and mast cells by mizolastine," Clin. Exp. Allergy, 34:241-9. reprint.pdf
Rounault, M., Bollinger, J.G., Lazdunski, M., Gelb, M.H., Lambeau, G. (2003) "Novel Mammalian Group XII Secreted Phospholipase A2 Lacking Enzymatic Activity," Biochemistry, 42:11494-11503. reprint.pdf
Beck, S., Lambeau, G., Scholz-Pedretti, K., Gelb, M.H., Janssen, M.J.W., Edwards, S.H., Wilton, D.C., Pfeilschifter, J., and Kaszkin, M. (2003) "Potentiation of TNFa-induced sPLA2-IIA expression in mesangial cells by an autocrine loop involving secreted phospholipase A2 and PPARa activation," J. Biol. Chem., 278:29799-29812. reprint.pdf
Gurrieri, S., Fürstenberger, G., Schadow, A., Haas, U., Singer, A.G., Ghomashchi, F., Pfeilschifter, J., Lambeau, G., Gelb, M.H., and Kaszkin, M. (2003) "Differentiation-dependent regulation of secreted phospholipases A2 in murine epidermis," J. Invest. Dermatol., 121:156-164. reprint.pdf
Poi, M.J., Tomaszewski, J.W., Yuan, C., Dunlap, C.A., Andersen, N.H., Gelb, M.H., and Tsai, M.-D. (2003) "A low-barrier hydrogen bond between histidine of secreted phospholipase A2 and a transition state analog inhibitor," J. Mol. Biol., 329:997-1009. reprint.pdf
Beers, S.A., Buckland, A.G., Giles, N., Gelb, M.H. and Wilton, D.C. (2003) "Effect of tryptophan insertions on the properties of the human group IIA phospholipase A2: mutagenesis produces an enzyme with characteristics similar to those of the human group V phospholipase A2," Biochem., 42(24):7326-7338. reprint.pdf
Murakami, M., Masuda, S., Shimbara, S., Bezzine, S., Lazdunski, M., Lambeau, G., Gelb, M.H., Matsukura, S., Kokubu, F., Adachi, M. and Kudo, I. (2003) "Cellular Arachidonate-Releasing Function of Novel Classes of Secretory Phospholipase A2s (Group III and XII)," J. Biol. Chem., 278:10657-10667. reprint.pdf
Singer, A.G., Ghomashchi, F., Le Calvez, C., Bollinger, J., Bezzine, S., Rouault, M., Sadilek, M., Lazdunski, M., Lambeau, G., and Gelb, M.H. (2002) "Interfacial Kinetic and Binding Properties of the Complete set of Human and Mouse Groups I, II, V, X, and XII Secreted Phospholipases A2," J. Biol. Chem., 277:48535-48549. reprint.pdf
Bezzine, S., Bollinger, J.G., Singer, A.G., Veatch, S.L., Keller, S.L., and Gelb, M.H. (2002) "On the Binding Preference of Human Groups IIA and X Phospholipases A2 for Membranes with Anionic Phospholipids," J. Biol. Chem., 277:48523-48534. reprint.pdf
Koprivnjak, T., Peschel, A., Gelb, M.H., Liang, N.S., and Weiss, J.O. (2002) "Role of charge properties of bacterial envelope in bactericidal action of human Group IIA phospholipase A2 against Staphylococcus andreas," J. Biol. Chem., 277:47636-47644. reprint.pdf
Murakami, M., Yoshihara, K., Shimbara, S., Lambeau, G., Gelb, M.H., Singer, A.G., Sawada, M., Inagaki, N., Nagai, H., Ishihara, M., Ishikawa, Y., Ishii, T., and Kudo, I. (2002) "Cellular Arachidonate-releasing Function and Inflammation-associated Expression of Group IIF Secretory Phospholipase A2," J. Biol. Chem., 277(21):19145-19155. reprint.pdf
Pan, Y.H., Yu, B.-Z., Singer, A.G., Ghomashchi, F., Lambeau, G., Gelb, M.H., Jain, M.J., and Bahnson, B.J. (2002) "Crystal structure of human group-X secreted phospholipase A2; electrostatically neutral interfacial binding surface targets zwitterionic membranes," J. Biol. Chem., 277:29086-29093. reprint.pdf
Canaan, S., Nielsen, R., Ghomashchi, F., Robinson, B.H., and Gelb, M.H. (2002) "Unusal Mode of Binding of human group-IIA secreted phospholipase A2 to anionic interfaces as studied by continuous wave and time domain electron paramagnetic resonance spectroscopy," J. Biol. Chem, 277:30984-30990. reprint.pdf
Murakami, M., Yoshihara, K., Shimbara, S., Lambeau, G., Singer, A., Gelb, M.H., Sawada, M., Inagaki, N., Nagai, H., and Kudo, I. (2002) "Arachidonate release and eicosanoid generation by group IIE phospholipase A2," Biochem. Biophys. Res. Comm., 292(3):689-696. reprint.pdf
Degousee, N., Ghomashchi, F., Stefanski, E., Singer, A., Smart, B.P., Borregaard, N., Reithmeier, R., Lindsay, T.F., Lichtenberger, C., Reinisch, W., Lambeau, G., Arm, J., Tischfield, J.T., Gelb, M.H., and Rubin, B.B. (2001) "Groups IV, V and X Phospholipases A2 in Human Neutrophils," J. Biol. Chem., 277(7):5061-5073. reprint.pdf
Beers, S.A., Buckland, A.G., Koduri, R.S., Cho, W., Gelb, M.H., and Wilton, D.C. (2002) "The antibacterial properties of secreted phospholipases A2: a major physiological role for the group IIA enzyme that depends on the very high pI of the enzyme to allow penetration of the bacterial cell wall," J. Biol. Chem., 277(3):1788-1793. reprint.pdf
Koduri, R.S., Grönroos, J.O., Laine, V.J.O., Calvez, C.L., Lambeau, G., Nevalainen, T.J., and Gelb, M.H. (2002) "Bactericidal Properties of Human and Murine Groups I, II, V, X, and XII Secreted Phospholipases A2," J. Biol. Chem., 277:5849-5857. reprint.pdf
Berg, O.G., Gelb, M.H., Tsai, M.-D., and Jain, M.K. (2001) "Interfacial Enzymology: The Secreted Phospholipase A2-Paradigm," Chem. Rev., 101:2613-2653. reprint.pdf
Muthalif, M.M., Hefner, Y., Canaan, S., Harper, J., Zhou, H., Parmentier, J.-H., Aebersold, R., Gelb, M.H., and Malik, K.U. (2001) "Functional Interacton of Calcium/Calmodulin-Dependent Protein Kinase II and Cytosolic Phospholipase A2." J. Biol. Chem., 276: 39653-39660. reprint.pdf
Ghomaschi, F., Stewart, A., Hefner, Y., Ramanadham, S., Turk, J., Leslie, C.C., and Gelb, M.H. (2001) "A pyrrolidine-based specific inhibitor of cytosolic phospholipase A2[alpha] blocks arachidonic acid release in a variety of mammalian cells." Biochimica et Biophysica Acta. 78118:1-7. reprint.pdf
Min, J.H., Wilder, C., Aoki, J., Arai, H., Inoue, K., Paul, L., and Gelb, M.H. (2001) "Platelet-Activating Factor Acetylhydrolases: Broad Substrate Specificity and Lipoprotein Binding Does Not Modulate the Catalytic Properties of the Plasma Enzyme." Biochemistry. 40(15):4539-4549. reprint.pdf
Schadow, A., Scholz-Pedretti, K., Lambeau, G., Gelb, M.H., Furstenberger, G., Pfeilschifter, J., and Kaszkin, M. (2001) "Characterization of group X phospholipase A2 as the major enzyme secreted by human keratinocytes and its regulation by the phorbol ester TPA," J. Investig. Dermatol., 116(1):31-39. reprint.pdf
Valentin E, Singer AG, Ghomashchi F, Lazdunski M, Gelb MH, Lambeau G. Cloning and Recombinant Expression of Human Group IIF-Secreted Phospholipase A(2). Biochem Biophys Res Commun. 279:223-228. reprint.pdf
Mounier CM, Luchetta P, Lecut C, Koduri RS, Faure G, Lambeau G, Valentin E, Singer A, Ghomashchi F, Beguin S, Gelb MH, Bon C. Related Articles, Links Basic residues of human group IIA phospholipase A2 are important for binding to factor Xa and prothrombinase inhibition comparison with other mammalian secreted phospholipases A2. Eur J Biochem. 2000 Aug;267(16):4960-4969. reprint.pdf
M. Murakami, R. S. Koduri, A. Enomoto, S. Shimbara, M. Seki, K. Yoshihara, A. Singer, E. Valentin, F. Ghomashchi, G. Lambeau, M.H. Gelb, and I. Kudo (2000) Distinct Arachidonate-Releasing Functions of Mammalian Secreted Phospholipase A2s in Human Embryonic Kidney 293 and Rat Mastocytoma RBL-2H3 Cells through Heparin Sulfate Shuttling and External Plasma Membrane Mechanisms, J. Biol. Chem., 276(13):10083-10096. reprint.pdf
Ying Hefner, Angelika G. Borsch-Haubold, Makomoto Murakami, Jonathan I. Wilde, Sophie Pasquet, David Schieltz, Farideh Ghomashchi, John R. Yates, III,Christopher G. Armstrong, Andrew Paterson, Philip Cohen, Rikiro Fukunaga, Tony Hunter, Ichiro Kudo, Steve P. Watson, and Michael H. Gelb. Serine-727 phosphorylation and activation of cytosolic phospholipase A2 by MNK1-related protein kinases J. Biol. Chem., 275:37543-37551 reprint.pdf
Enomoto A., Murakami, M., Valentin, E., Lambeau, G. Gelb, M. H. and Kudo, I; Redundant and Segregated Functions of Granule-Associated Heparin-Binding Group II Subfamily of Secretory Phospholipases A2 in the Regulation of Degranulation and Prostaglandin D2 Synthesis in Mast Cells: J. Immunol. 4007-4014 (2000). reprint.pdf
Gelb MH, Min J, Jain MK. (2000) Do membrane-bound enzymes access their substrates from the membrane or aqueous phase: interfacial versus non-interfacial enzymes. Biochim Biophys Acta. 2000 Oct 31; 1488(1-2):20-27. reprint.pdf
Linda S. Jung, Jennifer S. Shumaker-Parry, Charles T. Campbell,* Sinclair S. Yee, and Michael H. Gelb* ; Quantification of Tight Binding to Surface-Immobilized Phospholipid Vesicles Using Surface Plasmon Resonance: Binding Constant of Phospholipase A2, Journal of the American Chemical Society; 2000; 122(17); 4177-4184. reprint.pdf
Gelb MH, Valentin E, Ghomashchi F, Lazdunski M, Lambeau G. Cloning and recombinant expression of a structurally novel human secreted phospholipase A2. J Biol Chem. 2000; 275(51); 39823-39826. reprint.pdf
Bezzine S, Koduri RS, Valentin E, Murakami M, Kudo I, Ghomashchi F, Sadilek M, Lambeau G, Gelb MH. Exogenously added human group X secreted phospholipase A(2) but not the group IB, IIA, and V enzymes efficiently release arachidonic acid from adherent mammalian cells. Journal of Biological Chemistry. 275(5): 3179-91, Feb 2000 reprint.pdf
Valentin E, Ghomashchi F, Gelb MH, Lazdunski M, Lambeau G. Novel secreted phospholipase A(2) with homology to the group III bee venom enzyme. Journal of Biological Chemistry. 275, 7492-7496, 2000 reprint.pdf
Valentin E, Ghomashchi F, Gelb MH, Lazdunski M, Lambeau G. On the diversity of secreted phospholipases A(2). Cloning,tissue distribution, and functional expression of two novel mouse group II enzymes. Journal of Biological Chemistry. 274(44):31195-202, Oct 1999 reprint.pdf
F. Ghomashchi, R. Loo, J. Balsinde, F. Bartoli, E. A. Dennis, and M. H. Gelb (1999) Trifluoromethyl ketones and methyl fluorophosphonates as inhibitors of group IV and VI Phospholipases A2: structure-function studies with vesicle, micelle and membrane assays. Biochim. Biophys. Acta 1420, 45-56. reprint.pdf
Min JH, Jain MK, Wilder C, Paul L, Apitz-Castro R, Aspleaf DC, Gelb MH. Membrane-bound plasma platelet activating factor acetylhydrolase acts on substrate in the aqueous phase. Biochemistry. 38(39): 12935-42, Sep 1999. reprint.pdf
Buschbeck M, Ghomashchi F, Gelb MH, Watson SP, Borsch-Haubold AG. Stress stimuli increase calcium-induced arachidonic acid release through phosphorylation of cytosolic phospholipase A2. Biochemical Journal. 344 Pt 2: 359-66, Dec 1999. reprint.pdf
Valentin E, Koduri RS, Scimeca JC, Carle G, Gelb MH, Lazdunski M, Lambeau G. Cloning and recombinant expression of a novel mouse-secreted phospholipase A2. J Biol Chem. 1999 Jul 2;274(27):19152-60. reprint.pdf
Gelb, MH, Cho, W, and Wilton, DC. (1999) "Interfacial binding of secreted phospholipases A2: more than electrostatics and a major role for tryptophan," Curr. Opinion in Struct. Biol., 9(4):428-432. reprint.pdf
Han SK, Kim KP, Koduri R, Bittova L, Munoz NM, Leff AR, Wilton DC, Gelb MH, Cho W. Roles of Trp31 in high membrane binding and proinflammatory activity of human group V phospholipase A2. J Biol Chem 274(17): 11881-8, Apr 1999. reprint.pdf
Ball A; Nielsen R; Gelb MH; Robinson BH. Interfacial membrane docking of cytosolic phospholipase A2 C2 domain using electrostatic potential-modulated spin relaxation magnetic resonance.. Proc Natl Acad Sci U S A. 96(12): 6637-42, 8 Jun 1999. reprint.pdf
Hixon MS, Ball A, Gelb MH. Calcium-dependent and -independent interfacial binding and catalysis of cytosolic group IV phospholipase A2. Biochemistry. 1998 Jun 9;37(23):8516-26. reprint.pdf
Ghomashchi F;Lin Y;Hixon M S;Yu B Z;Annand R;Jain M K;Gelb M H. Interfacial recognition by bee venom phospholipase A2: insights into nonelectrostatic molecular determinants by charge reversal mutagenesis.. Biochemistry. 37(19): 6697-710, 12 May 1998. reprint.pdf
Lin Y; Nielsen R;Murray D;Hubbell W L;Mailer C;Robinson B H;Gelb M H. Docking phospholipase A2 on membranes using electrostatic potential-modulated spin relaxation magnetic resonance. Science. 279(5358): 1925-9, 20 Mar 1998 reprint.pdf
Koduri RS, Baker SF, Snitko Y, Han SK, Cho W, Wilton DC, Gelb MH. Action of human group IIa secreted phospholipase A2 on cell membranes. Vesicle but not heparinoid binding determines rate of fatty acid release by exogenously added enzyme. J Biol Chem. 273(48): 32142-53, Nov 1998. reprint.pdf
Borsch-Haubold AG, Bartoli F, Asselin J, Dudler T, Kramer RM, Apitz-Castro R, Watson SP, Gelb MH. Identification of the phosphorylation sites of cytosolic phospholipase A2 in agonist-stimulated human platelets and HeLa cells. J Biol Chem. 1998 Feb 20;273(8):4449-58. reprint.pdf
Nicolas JP, Lin Y, Lambeau G, Ghomashchi F, Lazdunski M, Gelb MH. Localization of structural elements of bee venom phospholipase A2 involved in N-type receptor binding and neurotoxicity. J Biol Chem. 1997 Mar 14;272(11):7173-81. reprint.pdf
Snitko Y, Koduri RS, Han SK, Othman R, Baker SF, Molini BJ, Wilton DC, Gelb MH, Cho W. Mapping the interfacial binding surface of human secretory group IIa phospholipase A2. Biochemistry. 1997 Nov 25;36(47):14325-33. reprint.pdf
Yu B Z;Ghomashchi F;Cajal Y;Annand R R;Berg O G;Gelb M H;Jain M K. Use of an imperfect neutral diluent and outer vesicle layer scooting mode hydrolysis to analyze the interfacial kinetics, inhibition, and substrate preferences of bee venom phospholipase A2. Biochemistry. 36(13): 3870-81, 1 Apr 1997. reprint.pdf
Annand RR, Kontoyianni M, Penzotti JE, Dudler T, Lybrand TP, Gelb MH. Active site of bee venom phospholipase A2: the role of histidine-34, aspartate-64 and tyrosine-87. Biochemistry. 1996 Apr 9;35(14):4591-601. reprint.pdf
de Carvalho MG, McCormack AL, Olson E, Ghomashchi F, Gelb MH, Yates JR 3rd, Leslie CC. Identification of phosphorylation sites of human 85-kDa cytosolic phospholipase A2 expressed in insect cells and present in human monocytes. J Biol Chem. 1996 Mar 22;271(12):6987-97. reprint.pdf
Bayburt T;Gelb M H. Interfacial catalysis by human 85 kDa cytosolic phospholipase A2 on anionic vesicles in the scooting mode. Biochemistry. 36(11): 3216-31, 18 Mar 1997. reprint.pdf
Protein Prenylation Reprints back to top
Bulbule, VJ, Rivas, K, Verlinde, CL, Van Voorhis, WC, Gelb, MH, (2008) "2-oxotetrahydroquinoline-based antimalarials with high potency and metabolic stability," J. Med. Chem., 51:384-387. reprint.pdf
Olepu, S, Suryadevara, PK, Rivas, K, Yokoyama, K, Verlinde, CL, Chakrabarti, D, Van Voorhis, WC, Gelb, MH, (2008) “2-Oxo-tetrahydro-1,8-naphthyridines as selective inhibitors of malarial protein farnesyltransferase and as anti-malarials,” Bioorg Med Chem Lett., 18:494-497. reprint.pdf
Yokoyama, K, Gillespie, JR, Van Voorhis, WC, Buckner FS, Gelb, MH, (2008) “Protein geranylgeranyltransferase-I of Trypanosoma cruzi,” Mol Biochem Parasitol, 157:32-43. reprint.pdf
Gillespie, JR, Yokoyama, K, Lu, K, Eastman, RT, Bollinger, JG, Van Voorhis, WC, Gelb, MH, Buckner, FS (2007) “C-terminal proteolysis of prenylated proteins in trypanosomatids and RNA interference of enzymes required for the post-translational processing pathway of farnesylated proteins,” Mol. Biochem. Parasitol., 153:115-124. reprint.pdf
Gelb, MH (2007) “Drug discovery for malaria: a very challenging and timely endeavor,” Current Opinion in Chemical Biology, 11:440-445. reprint.pdf
Van Voorhis, WC, Rivas, KL, Bendale, P, Nallan, L, Horney, C, Barrett, LK, Bauer, KD, Smart, BP, Ankala, S, Hucke, O, Verlinde, CL, Chakrabarti, D, Strickland, C, Yokoyama, K, Buckner, FS, Hamilton, AD, Williams, DK, Lombardo, LJ, Floyd, D, gelb, MH (2007) “Efficacy, pharmacokinetics, and metabolism of tetrahydroquinoline inhibitors of plasmodium falciparum protein farnesyltransferase,” Antimicrob. Agents Chemother., 10:3659-3671. reprint.pdf
Bendale, P, Olepu, S, Suryadevara, PK, Bulbule, V, Rivas, K, Nallan, L, Smart, B, Yokoyama, K, Ankala, S, Pendyala, PR, Floyd, D, Lombardo, LJ, Williams, DK, Buckner, FS, Chakrabarti, D, Verlinde, CL, Van Voorhis, WC, Gelb, MH (2007) “Second generation tetrahydroquinoline-based protein farnesyltransferase inhibitors as antimalarials,” J. Med. Chem., 50:4585-4605. reprint.pdf
Gelb, M.H., Brunsveld, L., Hrycyna, C.A., Michaelis, S., Tamanoi, F. , Van Voorhis, W.C. and Waldmann, H. (2006) "Therapeutic intervention based on protein prenylation and associated modifications," Nature Chem. Biol. 10: 518-528 reprint.pdf
Eastman, R.T., Buckner, F.S., Yokoyana, K., Gelb, M.H. and Van Voorhis, W.C. (2006) "Thematic review series: lipid posttranslational modifications. Fighting parasitic disease by blocking protein farnesylation," J. Lipid Res. 47: 233-40 reprint.pdf
Buckner, F.S., Eastman, R.T., Yokoyama, K., Gelb, M.H., Van Voorhis, W.C. (2005) " Protein farnesyl transferase inhibitors for the treatment of malaria and African trypanosomiasis," Curr Opin Investig Drugs 6:791-7 reprint.pdf
Yang, S.H., Bergo, M.O., Toth, J.I., Qiao, X., Hu, Y., Sandoval, S., Meta, M., Bendale, P., Gelb, M.H., Young, S.G., Fong, L.G. (2005) "Blocking protein farnesyltransferase improves nuclear blebbing in mouse fibroblasts with a targeted Hutchinson-Bilford progeria syndrome mutation," Prof. Natl. Acad. Sci 102:10291-6 reprint.pdf
Mallampalli, M.P., Huyer, G., Bendale, P., Gelb, M.H. and Michaelis, S. (2005) "Inhibiting farnesylation reverses the nuclear morphology defect in a HeLa cell model for Hutchinson-Gilford progeria syndrome," Proc. Natl. Acad. Sci. 102:14416-21 reprint.pdf
Toth, J.I., Yang, S.H., Qiao, X., Beigneux, A.P., Gelb, M.H., Moulson, C.L., Miner, J.H., Young, S.G. and Fong, L.G. (2005) "Blocking protein farnesyltransferase improves nuclear shape in fibroblasts from humans with progeroid syndromes," Proc. Natl. Acad. Sci. 102:12873-8 reprint.pdf
Hucke, O., Gelb, MH., Verlinde, CLMJ. et al. (2005) "The protein farnesyltransferase inhibitor tipifarnib as a new lead for the development of drugs against Chagas disease," J. Med. Chem., 48: 5415-5418. reprint.pdf
Glenn, MR., Chang, SY., Hucke, O., et al. (2005) "Structurally simple farnesyltransferase inhibitors arrest the growth of malaria parasites," Angew. Chem. Int. Ed. 41: 4903-4906. reprint.pdf
Toth, JI., Yang, SH., Qiao, X., et al. (2005) "Blocking protein farnesyltransferase improves nuclear shape in fibroblasts from humans with progeroid syndromes," Proc. Natl. Acad. Sci. 102:12873-12878. reprint.pdf
Eastman, RT., White, J., Hucke, O., et al. (2005) "Resistance to a protein farnesyltransferase inhibitor in Plasmodium falciparum," J. Biol. Chem. 280:13554-13559. reprint.pdf
Yang, S.H., Bergo, M.O., Toth, J.L., Qiao, X., Hu, Y., Sandoval, S., Meta, M., Bendale, P., Gelb, M.H., Young, S.G. and Fong, L.G. (2005) "Blocking protein farnesyltransferase improves nuclear blebbing in mouse fibroblasts with a targeted Hutchinson-Gilford progeria syndrome mutation," Proc. Natl. Acad. Sci. 109:10291-10296. reprint.pdf
Nallan, L., Bauer, K.D., Bendale, P., Rivas, K., Yokoyama, K., Horney, C.P., Pendyala, P.R., Floyd, D., Lombardo, L.J., Williams, D.K., Hamilton, A., Sebti, S., Windsor, W.T., Weber, P.C., Buckner, F.S., Chakrabarti, D., Gelb, M.H. and Van Voorhis, W.C. (2005) "Protein Farnesyltransferase Inhibitors Exhibit Potent Antimalarial Activity," J. Med. Chem., 48:3704-3713. reprint.pdf data supplement.pdf
Carrico, D., Ohkanda, J., Kendrick, H., Yokoyama, K., Blaskovich, M.A., Bucher, C.J., Buckner, F.S., Van Voorhis, W.C., Chakrabarti, D., Croft, S.L., Gelb, M.H., Sebti, S.M. and Hamilton, A.D. (2004) "In vitro and in vivo antimalarial activity of peptidomimetic protein farnesyltransferase inhibitors with improved membrane permeability," Bioorg Med Chem., 12:6517-26. reprint.pdf
Ohkanda, J., Buckner, F.S, Lockman, J.W., Yokoyama, K., Carrico, D., Eastman, R., Luca-Fradley, K., Davies, W., Croft, S.L., Van Voorhis, W.C., Gelb, M.H., Sebti, S.M., and Hamilton, A.D. (2004) "Design and Sythesis of Peptidominetic Protein Farnesyltransferase Inhibitors as Anti-Typanosoma brucei Agents,"J. Med. Chem., 47:432-445. reprint.pdf
Buckner, F., Yokoyama, K., Lockman, J., Aikenhead, K., Ohkanda, J., Sadilek, M., Sebti, S., Van Voorhis, W., Hamilton, A., and Gelb, M.H. (2003) "A class of sterol 14-demethylase inhibitors as anti-Trypanosoma cruzi agents," PNAS, 100:15149-15153. reprint.pdf
Ferri, N., Yokoyama, K., Sadilek, M., Paoletti, R., Apitz-Castro, R., Gelb, M.H., and Corsini, A. (2003) "Ajoene, a garlic compound, inhibits protein prenylation and arterial smooth muscle cell proliferation," Br. J. Pharmacol., 138:811-818. reprint.pdf
Clerici, F., Gelmi, M.L., Yokoyama, K., Pocar, D., Van Voorhis, W.C., Buckner, F.S., and Gelb, M.H. (2002) "Isothiazole Dioxides: Synthesis and Inhibition of Trypanosoma brucei Protein Farnesyltrasnferase," Bioorg. Med. Chem. Lett., 12:2217-2220 reprint.pdf
Buckner, F.S., Eastman, R.T., Nepomuceno-Silva, J.L., Speelmon, E.C., Myler, P.J., Van Voorhis, W.C., and Yokoyama, K. (2002) "Cloning, heterologous expression, and substrate specificities of protein farnesyltransferases from Trypanosoma cruzi and Leishmania major," Molec. & Biochem. Parasitol., 122:181-188. reprint.pdf
Ferri, N., Arnaboldi, A., Orlandi, A., Yokoyama, K., Gree, R., Granata, A., Hachem, A., Paoletti, R., Gelb, M.H., and Corsini, A. (2001) "Effect of S(-) perillic acid on protein prenylation and arterial smooth muscle cell proliferation," Biochem. Pharm., 62:1637-1645. reprint.pdf
Nepomuceno-Silva, J.L., Yokoyama, K., de Mello, L.D.B., Mendonca, S.M., Paixao, J.C., Baron, R., Faye, J.-C., Buckner, F.S., Voorhis, W.C.V., Gelb, M.H., and Lopes, U.G. (2001) "TcRho1, a Farnesylated Rho Family Homologue from Trypanosoma cruzi," J. Biol. Chem., 276:29711-29718. reprint.pdf
Ohkanda, J., Lockman, J.W., Yokoyama, K., Gelb, M.H., Croft, S., Kendrick, H., Harrell, M.I., Feagin, J.E., Blaskovich, M.A., Sebti, S.M., and Hamilton, A.D. (2001) "Peptidomimetic inhibitors of protein farnesyltransferase show potent antimalarial activity," Bioorg. Med. Chem. Lett., 11:761-764. reprint.pdf
Terry A. Cook, Farideh Ghomashchi, Michael H. Gelb, Stephanie K. Florio, and Joseph A. Beavo. The delta subunit of type 6 phosphodiesterase reduces light-induced cGMP hydrolysis in rod outer segments J. Biol. Chem., 276:5248-5255. reprint.pdf
Cook TA, Ghomashchi F, Gelb MH, Florio S, Beavo JA Binding of the delta subunit to rod phosphodiesterase catalytic subunits requires methylated, prenylated C-termini of their catalytic subunits. Biochemistry 2000. reprint.pdf
Buckner FS, Yokoyama K, Nguyen L, Grewal A, Erdjument-Bromage H, Tempst P, Strickland CL, Xiao L, Van Voorhis WC, Gelb MH. Cloning, heterologous expression, and distinct substrate specificity of protein farnesyltransferase from Trypanosoma brucei. J Biol Chem. 2000 Jul 21;275(29):21870-6. reprint.pdf
Yokoyama K, Trobridge P, Buckner FS, Van Voorhis WC, Stuart KD, Gelb MH. Protein farnesyltransferase from Trypanosoma brucei. A heterodimer of 61- and 65-kda subunits as a new target for antiparasite therapeutics. Journal of Biological Chemistry. 273(41): 26497-505, Oct 1998. reprint.pdf
Jang, G. F. and Gelb, M. H. (1998) "Substrate specificity of mammalian prenyl protein-specific endoprotease activity," Biochemistry. 37(13): 4473-81, reprint.pdf
Yokoyama, K., Trobridge, P., Buckner, F.S., Scholten, J., Stuart, K.D., Voorhis, W.C.V., and Gelb, M.H. (1998) "The effects of protein farnesyltransferase inhibitors on trypanosomatids: inhibition of protein farnesylation and cell growth." Molecular and Biochemical Parasitology, 94:87-97. reprint.pdf
Yokoyama, K., Lin, Y., Stuart, K.D., and Gelb, M.H. (1997) "Prenylation of proteins in Trypanosoma brucei." Molecular & Biochemical Parasitology, 87:61-69. reprint.pdf
Yokoyama, K., Zimmerman, K., Scholten, J. and Gelb M. H. (1997) "Differential prenyl pyrophosphate binding to mammalian protein geranylgeranyltransferase-I and protein farnesyltransferase and its consequence on the specificity of protein prenylation,"
J Biol Chem 272: 3944-52 reprint.pdf
Dudler T. and Gelb M.H. (1996) "Palmitoylation of Ha-Ras facilitates membrane binding, activation of downstream effectors, and meiotic maturation in Xenopus oocytes,"
J Biol Chem. 271:11541-7. reprint.pdf
McGeady P;Kuroda S;Shimizu K;Takai Y;Gelb M H. (1995) "The farnesyl group of H-Ras facilitates the activation of a soluble upstream activator of mitogen-activated protein kinase,"
J Biol Chem. 270: 26347-51 reprint.pdf
Anti-Parasite Drug Design (other than protein prenylation) Reprints back to top
Ojo, Kayode K. , Gillespie, J. Robert, Reichers, Aaron J. , Napuli, Alberto J., Verlinde, Christophe L. M. J., Buckner, Frederick S., Gelb, Michael H., Domostoj, Mathias M., Wells, Susan J., Scheer, Alexander, Wells, Timothy N. C., and Van Voorhis, Wesley C. (2008) "Glycogen synthase kinase 3 is a potential drug target for African trypanosomiasis therapy," Antimicrobial Agents and Chemotherapy 52:3710-3717. reprint.pdf
Mehlin, C., Boni, E., Buckner, F.S., Engel, L., Feist, T., Gelb, M.H., Haji, L., Kim, D., Liu, C., Mueller, N., Myler, P.J., Reddy, J.T., Sampson, J.N., Subramanian, E., Van Voorhis, W.C., Worthey, E., Zucker, F., Hol, W.G. (2006) "Heterologous expression of proteins from Plasmodium falciparum: results from 1000 genes,"
Mol Biochem Parasitol. 148:144-60. reprint.pdf
Eastman, R.T., Buckner, F.S., Yokoyana, K., Gelb, M.H. and Van Voorhis, W.C. (2006) "Thematic review series: lipid posttranslational modifications. Fighting parasitic disease by blocking protein farnesylation," J. Lipid Res. 47: 233-40 reprint.pdf
Gelb, M.H., Van Voorhis, W.C., Buckner, F.S., Yokoyama, K., Eastman, R., Carpenter, E.P., Panethymitaki, C., Brown, K.A., and Smith, D.F. (2003) "Protein farnesyl and N-myristoyl transferases: piggy-back medicinal chemistry targets for the development of antitrypanosomatid and antimalarial therapeutics," Molec. Biochem. Parasitol., 126:155-163. reprint.pdf
Choe, J., Suresh, S., Wisedchaisri, G., Kennedy, K.J., Gelb, M.H. and Hol, W.G.J. (2002) "Anomalous differences of light elements in determining precise binding modes of ligands to glycerol-3-phosphate dehydrogenase," Chem. Biol., 9:1189-1197. reprint.pdf
Gelb, M.H. and Hol, W.J.G. (2002) "Drugs to combat tropical protozoan parasites," Science, 297:343-344. reprint.pdf
Suresh, S., Bressi, J.C., Kennedy, K.J., Verlinde, C.L.M.J., Gelb, M.H., and Hol, W.G.J. (2001) "Conformational Changes in Leishmania mexicana Glyceraldehyde-3-phosphate Dehydrogenase Induced by Designed Inhibitors," J. Mol. Biol., 309:423-435. reprint.pdf
Bressi, J.C., Verlinde, C.L.M.J., Aronov, A.M., Shaw, M.L., Shin, S.S., Nguyen, L.N., Suresh, S., Buckner, F.S., Van Voorhis, W.C., Kuntz, I.D., Hol, W.G.J., and Gelb, M.H. Adenosine Analogues as Selective Inhibitors of Glyceraldehyde-3-phosphate Dehydrogenase of Trypanosomatidae via Structure-Based Drug Design. J. Med. Chem, 44(13):2080-2093. reprint.pdf
Verlinde, CLMJ, Hannaert, V, Blonski, C, Willson, M, Perie, JJ, Fothergill-Gilmore, LA, Opperdoes, FR, Gelb, MH, Hol, WGJ, amd Michels, PAM. (2001) "Glycolysis as a target for the design of new anti-trypanosome drugs," Drug Res. Updates, 4:1-14.reprint.pdf
Kennedy, K. J., Bressi, J. C., and Gelb, M. H. A Disubstituted NAD+ Analogue is a Nanomolar Inhibitor of Trypanosomal Glyceraldehyde-3-phosphate Dehydrogenase, Bioorg. Med. Chem. Lett., 11(2):95-98. reprint.pdf
Bressi JC, Choe J, Hough MT, Buckner FS, Van Voorhis WC, Verlinde, CLMJ, Hol, WGJ, Gelb MH. Adenosine analogues as inhibitors of Trypanosoma brucei phosphoglycerate kinase: Elucidation of a novel binding mode for a 2-amino-N6-substituted adenosine. J. Med. Chem. 2000 reprint.pdf
Golisade A, Bressi JC, Van Calenbergh S, Gelb MH, Link A. Polymer-Assisted Solution-Phase Synthesis of 2'-Amido-2'-deoxyadenosine Derivatives Targeted at the NAD(+)-Binding Sites of Parasite Enzymes. J Comb Chem. 2000 Sep 12;2(5):537-544. reprint.pdf
Aronov AM; Suresh S; Buckner FS; Van Voorhis WC; Verlinde CL; Opperdoes FR; Hol WG; Gelb MH. Structure-based design of submicromolar, biologically active inhibitors of trypanosomatid glyceraldehyde-3-phosphate dehydrogenase. Proc Natl Acad Sci U S A. 96(8): 4273-8, 13 Apr 1999. reprint.pdf
Aronov AM, Verlinde CL, Hol WG, Gelb
MH. Selective tight binding inhibitors of trypanosomal
glyceraldehyde-3-phosphate dehydrogenase via structure-based drug
design. J Med Chem. 41(24): 4790-9, Nov 1998. reprint.pdf
Clinical Enzymology and Quantitative Proteomics Reprints back to top
Wang, Y, Scott, CR, Gelb, MH, Turecek, F (2008) "Direct Assay of Enzymes in Heme Biosynthesis for the Detection of Prophyrias by Tandem Mass Spectrometry," Anal. Chem. reprint.pdf
Wang, Y, Gatti, P, Sadilek, M, Scott, CR, Turecek, F, Gelb, MH (2008) "Direct Assay of Enzymes in Heme Biosynthesis for the Detection of Porphyrias by Tandem Mass Spectrometry. Uroporphyrinogen Decarboxylase and Coproporphyrinogen III Oxidase," Anal. Chem. reprint.pdf
Turecek, F, Scott, CR, Gelb, MH (2007) “Tandem mass spectrometry in the detection of inborn errors of metabolism for newborn screening,” Methods Mol. Biol., 359:143-157. reprint.pdf
Lu, Y, Bottari, P, Aebersold, R, Turecek, F, Gelb, MH (2007) “Absolute quantification of specific proteins in complex mixtures using visible isotope-coded affinity tags,” Methods Mol. Biol., 359:159-176. reprint.pdf
Wang, D., Wood,T., Sadilek, M., Scott, C.R., Turecek, F. and M. H. Gelb (2007) "Tandem Mass Spectrometry for the Direct Assay of Enzymes in Dried Blood Spots: Application to Newborn Screening for Mucopolysaccharidosis II (Hunter Disease)," Clin. Chem. 53:137 reprint.pdf
Gelb, M.H., Turecek, F., Scott, R.C., and Nestor A. Chamoles. (2006) " Direct multiplex assay of enzymes in dried blood spots by tandem mass spectrometry for the newborn screening of lysosomal storage disorders," J Inherit Metab Dis 29:397–404. reprint.pdf
Seebacher,J., Mallick, P., Zhang, N., Eddes J.S., Aebersold, R. and Michael H. Gelb (2006)" Protein Cross-Linking Analysis Using Mass Spectrometry, Isotope-Coded Cross-Linkers, and Integrated Computational Data Processing." Journal of Proteome Research, 5(9):2270-82. reprint.pdf
Scott, C.R. (2005) "Newborn Screening for Lysosomal Storage Disorders,"
Clinical Perspectives 12:11-15. reprint.pdf
Wang, D., Eadala, B., Sadilek, M., Chamoles N.A., Turecek, F., Scott, C.R. and Gelb, M.H. (2005) "Tandem Mass Spectrometric Analysis of Dried Blood Spots for Screening of Mucopolysaccharidosis I in Newborns," Clin. Chem. 51:898-900. reprint.pdf, supplement.pdf, editorial.pdf
Li, Y., Scott, C.R., Chamoles, N.A., Ghavami, A., Pinto, B.M., Turecek, F., Gelb, M.H. (2004) "Direct Multiplex Assay of Lysosomal Enzymes in Dried Blood Spots for Newborn Screening," Clin. Chem., 50:1785-96. reprint.pdf
Lu, Y., Bottari, P., Turecek, F., Aebersold, R. and Gelb, M.H. (2004) "Absolute Quantification of Specific Proteins in Complex Mixtures Using Visible Isotope-Coded Affinity Tags," Anal. Chem., 76:4104-11. reprint.pdf
Li, Y., Brockman, K., Turecek, F., Scott, C.R., Gelb, M.H. (2004) "Tandem Mass Spectrometry for the Direct Assay of Enzymes in Dried Blood Spots: Application to Newborn Screening for Krabbe Disease," Clin. Chem., 50: 638-640. reprint.pdf data supplement.pdf
Bottari, P., Aebersold, R., Turecek, F., and Gelb, M.H. (2004) "Design and Synthesis of Visible Isotope-Coded Affinity Tags for the Absolute Quantification of Specific Proteins in Complex Mixtures," Bioconj. Chem., ASAP article 10.1021/bc03417s. reprint.pdf
Li, Y., Ogata, Y., Freeze, H.H., Scott, C.R., Turecek, F., and Gelb, M.H. (2003) "Affinity Capture and Elution/Electrospray Ionization Mass Spectrometry Assay of Phosphomannomutase and Phosphomannose Isomerase for the Multiplex Analysis of Congenital Disorders of Glycosylation Types Ia and Ib," Anal. Chem., 75:42-48. reprint.pdf
Ogata, Y., Scampavia, L., Ruzicka, J., Scott, C.R., Gelb, M.H., and Turecek, F. (2002) "Automated Affinity Capture-Release of Biotin-Containing Conjugates Using a Lab-on-Valve Apparatus Coupled to UV/Visible and Electrospray Ionization Mass Spectrometry," Anal. Chem., 74:4702-4708. reprint.pdf
Gerber, S.A., Turecek, F., and Gelb, M.H. (2001) "Design and Synthesis of Substrate and Internal Standard Conjugates for Profiling Enzyme Activity in the Sanfilippo Syndrome by Affinity Chromatography/Electrospray Ionization Mass Spectrometry." Bioconj. Chem., 12(4): 603-315. reprint.pdf
Zhou, X., Turecek, F., Scott, C.R., and Gelb, M.H. Quantification of Cellular Acid Sphingomyelinase and Galactocerebroside B-Galactosidase Activities by Electrospray Ionization Mass Spectrometry. Clin. Chem., 2001, 47(5):874-881. reprint.pdf
Gerber, S.A., Scott, C.R., Turecek, F., Gelb, M.H. Direct Profiling of Multiple Enzyme Activities in Human Cell Lysates by Affinity Chromatography/Electrospray Ionization Mass Spectrometry: Application to Clinical Enzymology. Anal. Chem., 2001, 73:1651-1657. reprint.pdf
Gygi SP, Rist B, Gerber SA, Turecek F, Gelb MH, Aebersold R. Quantitative analysis of complex protein mixtures using isotope-coded affinity tags. Nat Biotechnol. 1999 Oct;17(10):994-9 reprint.pdf commentary.pdf
Gerber SA, Scott CR, Turecek F, Gelb
MH. Analysis of rates of muliple enzymes in cell lysates by
electrospray ionization mass spesctrometery. J. Am. Chem. Soc. 1999.
reprint.pdf
Other Reprints back to top
Gelb, M.H. and Holm, B.P. (2006) "A simple and inexpensive device for removal of solvent from a large collection of sample tubes," J Comb Chem. 8:15-27. reprint.pdf
Kuksa, V., Bartl, F., Maeda, T., Jang, G.-F., Ritter, E., Heck, M., Van Hooser, J.P., Liang, Y., Filipek, S., Gelb, M.H., Hofmann, K.P., and Palczewski, K. Biochemical and Physiological Properties of Rhodopsin Regenerated with 11-cis-6-Ring- and 7-Ring-Retinals. J. Biol. Chem., 2002, 277:42315-42324. reprint.pdf
Goldstein, A.S., Gelb, M.H., and Yager, P. The relationship between the structure of the headgroup of sphingolipids and their ability to form complex high zxial ratio microstructures. Chemistry and Physics of Lipids, 2001, 109:1-14. reprint.pdf
Goldstein, A.S., Gelb, M.H., and Yager, P. Continuous and highly variable rate controlled release of model drugs from sphingolipid-based complex high axial ratio microstructures. J. of Controlled Release, 2001, 70:125-138. reprint.pdf
Jang, G.F., Kuksa, V., Filipek, S., Bartl, F., Ritter, E., Gelb, M.H., Hofmann, K.P., and Palczewski, K. J. Biol. Chem. Mechanism of rhodopsin activation as examined with ring constrained retinal analogs and the crystal structure of the ground state protein. J. Biol. Chem., 2001, 276: 26148-26153. reprint.pdf
Stecher H; Gelb MH; Saari JC; Palczewski K. Preferential release of 11-cis-retinol from retinal pigment epithelial cells in the presence of cellular retinaldehyde-binding protein.. J Biol Chem. 274(13): 8577-85, 26 Mar 1999. reprint.pdf