Download pdf files of recent Gelb Group reprints from the list below.
 
Phospholipase Reprints
Protein Prenylation Reprints
Anti-Parasite Drug Design (other than protein prenylation) Reprints
Clinical Enzymology and Quantitative Proteomics Reprints
Other Reprints
 

Phospholipase Reprints   back to top

Sato, H., Taketomi, Y., Ushida, A., Isogai, Y., Kojima, T., Hirabayashi, T., Miki, Y., Yamamoto, K., Nishito, Y., Kobayashi, T., Ikeda, K., Taguchi, R., Hara, S., Ida, S., Miyamoto, Y., Watanabe, M., Baba, H., Miyata, K., Oike, Y., Gelb, M. H., Murakami, M., (2014) "The adipocyte-inducible secreted phospholipases PLA2G5 and PLA2G2E play distinct roles in obesity," Cell Met., 20:1-14.  PMID: 24910243  reprint

Kelvin, A. A., Degousee, N., Banner, D., Stefanski, E., Leon, A. J., Angoulvant, D., Paquette, S. G., Huang, S. S. H., Danesh, A., Robbins, C. S., Noyan, H., Husain, M., Lambeau, G., Gelb, M., Kelvin, D. J., Rubin, B. B. (2014) "Lack of group X secreted phospholipase A2 increases survival following pandemic H1N1 influenza infection," Virology, 454-455, 78-92. PMID:  24725934   reprint

Bollinger, J. G., Naika, G. S., Sadilek, M., Gelb, M. H. (2013) "LC/ESI-MS/MS detection of FAs by charge reversal derivatization with more than four orders of magnitude improvement in sensitivity,"J. Lipid Res., 54:3523-3530.  PMID:  23945566 reprint

Miki, Y., Yamamoto, K., Taketomi, Y., Sato, H., Shimo, K., Kobayashi, T., Ishikawa, Y., Ishii, T., Nakanishi, H., Ikeda, K., Taguchi, R., Kabashima, K., Arita, M., Arai, H., Lambeau, G., Bollinger, J. M., Hara, S., Gelb, M. H., Murakami, M. (2013) "Lymphoid tissue phospholipase A2 group IID resolves contact hypersensitivity by driving antiinflammatory lipid mediators," J. Exp Med., May 20, 2013, 1217-1234.  PMID:  23690440 reprint

Taketomi, Y., Ueno, N., Kojima, T., Sato, H., Murase, R., Yamamoto, K., Tanaka, S., Sakanaka, M. Nakamura, M., Nishito, Y., Kawana, M., Kambe, N., Ikeda, K., Taguchi, R., Nakamizo, S., Kabashima, K., Gelb, M. H., Arita, M., Yokomizo, T., Nakamura, M., Watanabe, K., Hirai, H., Nakamura, M., Okayama, Y., Ra, C., Aritake, K., Urade, Y., Morimoto, K., Sugimoto, Y., Shimizu, T., Narumiya, S., Hara, S., Murakami, M. "Mast cell maturation is driven via a group III phospholipase A2-prostaglandin D2-DP1 receptor paracrine axis" Nature Immunology, 14, 554-563, doi:10.1038/ni.2586  PMID:  23624557  reprint  supplemental

Lee, L.K., Bryant, K.J., Bouveret, R., Lei, P.W.,Duff, A.P., Harrop, S.J., Huang, E.P., Harvey, R.P., Gelb, M.H., Gray, P.P., Curmi, P.M., Cunningham, A.M., Church, W.B., Scott, K.F. (2013) "Selective inhibition of human group IIA secreted phospholipase A2 (hGIIA) signaling reveals arachidonic acid metabolism is associated with colocalization of hGIIA to vimentin in rheumatoid synoviocytes." Journal of Biological Chemistry.

PMID: 23482564  reprint

Hallstrand, T. S., Lai, Y., Ni, Z., Oslund, R. C., Henderson, W. R., Gelb, M. H., et al. (2012). "Relationship between levels of secreted phospholipase A2 groups IIA and X in the airways and asthma severity." Clinical & Experimental Allergy, 41(6), 801-810. PMID: 21255140.   reprint

Sandoval, G., Aloulou, A., Ali, Y., Bezzine, S., Gargouri, Y., & Gelb, M. (2012). Phospholipases: An Overview. In Lipases and Phospholipases (Vol. 861, pp. 63-85): Humana Press.  reprint

Henderson, H.R., Ye, X., Lai, Y., Bollinger, J.G., Tien, Y.T., Chi, E.Y., Gelb, M.H. (2012) " Key role of group V secreted phospholipase A2 in Th2 cytokine and dendritic cell-driven airway hyperresponsiveness and remodeling,"  PLoS ONE, 8:1-16.  PMID: 23451035 reprint

Oslund, R.C., Gelb, M.H. (2012) "Biochemical Characterization of Selective Inhibitors of Human Group
IIA Secreted Phospholipase A2 and Hyaluronic Acid-Linked Inhibitor Conjugates, " Biochemistry, 51: 8617−8626.  reprint

Thompson, W., Oslund, R.C., Bollinger, J.,  Ewing, H., and Gelb, M.H. (2012) "High-Throughput Assay of Secreted Phospholipases A2 Inhibitors." Chapter 10 in Lipases and Phospholipases: Methods and Protocols, Methods in Molecular Biology, Vol. 861, (Ed. Georgina Sandoval), pp. 149-158.  reprint

Hallstrand, T.S., Lai, Y., Henderson, Jr., W.R., Altemeier, W.A., Gelb, M.H. (2011) "Epithelial regulation of eicosanoid production in asthma," Pulmonary Pharmacology & Therapeutics, 25:432-437.  PMID: 2332327.  reprint

Yang, J.-S., Valente, C., Polishchuk, R. S., Turacchio, G., Layre, E., Branch Moody, D., et al. (2011). "COPI acts in both vesicular and tubular transport." Nat Cell Biol, 13(8), 996-1003. PMID: 21725317. reprint

Jemel, I., Ii, H., Oslund, R.C., Payre, C., Dabert-Gay, A.-S., Douguet, D., Chargui, K., Scarzello, S., Gelb, M. H., and Lambeau, G. (2011) "Group X secreted phospholipase A2 proenzyme is matured by a furin-like proprotein convertase and releases arachidonic acid inside of human HEK293 cells," J. Biol. Chem., 286:36509-36521.  PMID: 21878635.  reprint supp.

Bryant, K.J., Bidgood, M.J., Lei, P.W., Taberner, M., Salom, C., Kumar, V., Lee, L., Church, W. B., Courtenay, B., Smart, B. P., Gelb, M. H., Cahill, M.A., Graham, G. G., McNeil, M. P., Scott, K. F. (2011) "A bifunctional role for group IIA secreted phospholipase A2 in human rheumatoid fibroblast-like synoviocyte arachidonic acid metabolism," J. Biol. Chem., 286:2492-2503.  PMID: 21068383   reprint

Reed KA, Tucker DE, Aloulou A, Adler D, Ghomashchi F, Gelb MH, Leslie CC, Oates JA, Boutaud O. (2011) "Functional characterization of mutations in inherited human cPLAâ‚‚ deficiency." Biochemistry.  50(10):1731-8. PMID: 21247147.   reprint

Mouchlis, V.D., Magrioti, V., Barbayianni, E., Cermak, N., Oslund, R.C., Mavromoustakos, T.M., Gelb, M.H., Kokotos, G. (2011)  " Inhibition of secreted phospholipases A2  by 2-oxoamides based on  alpha-amino acids: Synthesis, in vitro evaluation and molecular docking calculations." Bioorg Med Chem.  19(2):735-43.

PMID: 21216150 reprint

Lai, Y., Oslund, R. C., Bollinger, J. G., Henderson, W. R. Jr., Santana, L. F., Altemeier, W. A., Gelb, M. H., Hallstrand, T. S. (2010) "Eosinophil cysteinyl leukotriene synthesis mediated by exogenous secreted phospholipase A2 group X," J. Biol. Chem., 285(53):41491-41500.  PMID:  20974857   reprint  supp.

Boilard, E., Lai, Y., Larabee, K., Balestrieri, B., Ghomashchi, F., Fujioka, D., Gobezie, R., Coblyn, J. S., Weinblatt, M. E., Massarotti, E. M., Thornhill, T. S., Divangahi, M., Remold, H., Lambeau, G., Gelb, M. H., Arm, J. P., Lee, D. M. (2010) "A novel anti-inflammatory role for secretory phospholipase A2 in immune complex-mediated arthritis," EMBO Mol. Med., 2(5):172-187.  PMID:  20432503 reprint

Shridas, P., Bailey, W. M., Boyanovsky, B. B., Oslund, R. C., Gelb, M. H., Webb, N. R. (2010) "Group X secretory phospholipase A2 regulates the expression of steroidogenic acute regulatory protein (StAR) in mouse adrenal glands," J. Biol. Chem., 285(26):20031-20039.  PMID:  20421306   reprint

Ghomashchi, F., Naika, G. S., Bollinger, J. G., Aloulou, A., Lehr, M., Leslie, C. C., Gelb, M. H. (2010) "Interfacial kinetic and binding properties of mammalian group IVB phospholipase A2 (cPLA2{beta}) and comparison with the other cPLA2 isoforms," J. Biol. Chem., 285(46):36100-36111.  PMID:  20705608   reprint  supp.

Zack, M., Boyanovsky, B. B., Shridas, P., Bailey, W., Forrest, K., Howatt, D. A., Gelb, M. H., de Beer, F. C., Daugherty, A., Webb, N. R. (2010) "Group X secretory phospholipase A(2) augments angiotensin II-induced inflammatory responses and abdominal aortic aneurysm formation in apoE-deficient mice," Atherosclerosis, 214:58-64.  PMID:  20833395   reprint

Bollinger, J. G., Thompson, W., Lai, Y., Oslund, R. C., Hallstrand, T. S., Sadilek, M., Turecek, F., Gelb, M. H. (2010) "Improved sensitivityi mass spectrometric detection of eicosanoids by charge reversal derivatization," Anal. Chem., 82:6790-6796.  PMID:  20704368 reprint supplement

Shridas, P., Bailey, W. M., Gizard, F., Oslund, R. C., Gelb, M. H., Bruemmer, D., Webb, N. R. (2010) "Group X secretory phospholipase A2 negatively regulates ABCA1 and ABCG1 expression and cholesterol efflux in macrophages," Arterioscler Thromb Vasc Biol., 30:2014-2021. PMID:  20844270   reprint supplement

Shridas, P., Bailey, W. M., Boyanovsky, B. B., Oslund, R. C., Gelb, M. H., Webb, N. R. (2010) "Group X secretory phospholipase A2 regulates the expression of steroidogenic acute regulatory protein (StAR) in mouse adrenal glands," J. Biol. Chem., 285:20031-20039.  PMID:  20421306   reprint

Granata, F., Frattini, A., Loffredo, S., Staiano, R. I., Petraroli, A., Ribatti, D., Oslund, R., Gelb, M. H., Lambeau, G., Marone, G., Triggiani, M. (2010) "Production of vascular endothelial growth factors from human lung macrophages induced by group IIA and group X secreted phospholipases A(2)," J. Immunol., 184:5232-5241.  PMID:  20357262   reprint

Hallstrand, T. S., Wurfel, M. M., Lai, Y., Ni, Z, Gelb, M. H., Altemeier, W. A., Beyer, R. P., Aitken, M. L., Henderson, W. R. (2010) "Transglutaminase 2, a novel regulator of eicosanoid production in asthma revealed by genome-wide expression profiling of distinct asthma phenotypes," PLoS One, 5(1):e8583.  PMID:  20052409   reprint

Surrel, F., Jemel, I., Boilard, E., Bollinger, J. G., Payre, C., Mounier, C. M., Talvinen, K. A., Laine, V. J., Nevalainen, T. J., Gelb, M. H., Lambeau, G. (2009) "Group X phospholipase A2 stimulates the proliferation of colon cancer cells by producing various lipid mediators," Mol. Pharmacol., 76:778-790.  PMID:  19602573   reprint

Leslie, C. C., Gangelhoff, T. A., Gelb, M. H. (2010) "Localization and function of cytosolic phospholipase A(2)alpha at the Golgi," Biochimie, 92:620-626.  PMID:  20226226  reprint

Olson, E. D., Nelson, J., Griffith, K., Nguyen, T., Streeter, M., Wilson-Ashworth, H. A., Gelb, M. H., Judd, A. M., Bell, J. D. (2010) "Kinetic evaluation of cell membrane hydrolysis during apoptosis by human isoforms of secretory phospholipase A2," J. Biol. Chem., 285:10993-11002.  PMID:  20139082   reprint  supplement

Bollinger, J. G., Ii, H., Sadilek, M. and Gelb, M. H., (2010) "Improved method for the quantification of lysophospholipids including enol ether species by liquid chromatography-tandem mass spectrometry," J. Lipid Res., 51:440-447.  PMID:  19717841 reprint   supp.

Rosenson, R. S., Gelb, M. H. (2009) "Secretory phospholipase A(2): A multifaceted family of proatherogenic enzymes," Curr. Cardiol. Rep., 11:445-451.  PMID:  19863869   reprint

Regan-Klapisz, E., Krouwer, V., Langelaar-Makkinje, M., Nallan, L., Gelb, M., Gerritsen, H., Verkleij, A. J. and Post, J. A. (2009) "Golgi-associated cPLA2alpha regulates endothelial cell-cell junction integrity by controlling the trafficking of transmembrane junction proteins", Molecular Biology of the Cell, 20:4225-4234.  PMID:  19675210   reprint

Surrel, F., Jemel, I., Boilard, E., Bollinger, J. G., Payre, C., Mounier, C. M., Talvinen, K. A., Laine, V. J., Nevalainen, T. J., Gelb, M. H., Lambeau, G. (2009) "Group X pospholipase A2 stimulates the proliferation of colon cancer cells by producing various lipid mediators," Mol. Pharmacol., 76:778-790.  PMID 19602573  reprint

Triggiani, M., Giannattasio, G., Calabrese, C., Loffredo, S., Granata, F., Fiorello, A., Santini, M., Gelb, M. H. (2009) "Lung mast cells are a source of secreted phospholipases A2", J. Allergy Clin Immunol., 124:558-565.  PMID   19541351 reprint

Balestrieri, B., Maekawa, A., Xing, W., Gelb, M.H., Katz, H.R., Arm, J.P. (2009) "Group V secretory phospholipase A2 modulates phagosome maturation and regulates the innate immune response against Candida albicans", J. Immunol., 182(8):4891-4898.  PMID:  19342668   reprint

Giannattasio, G., Lai, Y., Granata, F., Mounier, C.M., Nallan, L., Oslund, R., Leslie, C.C., Marone, G., Lambeau, G., Gelb, M.H., Triggiani, M. (2009) "Expression of phospholipases A(2) in primary human lung macrophages Role of cytosolic phospholipase A(2)-alpha in arachidonic acid release and platelet activating factor synthesis", Biochim Biophys Acta, 1791(2):92-102. PMID:  19130898   reprint

Tucker, D.E., Ghosh, M., Ghomashchi, F., Loper, R., Suram, S., St. John, B., Girotti, M., Bollinger, J.G., Gelb, M.H., Leslie, C.C. (2009) "Role of phosphorylation and basic residues in the catalytic domain of cytosolic phospholipase A2alpha in regulating interfacial kinetics and binding and cellular function, J. Biol. Chem., J. Biol. Chem, 284:9596-9611.  PMID:  19176526   reprint supplement

Tucker, D.E., Gijon, M.A., Spencer, D.M., Qiu, Z.H., Gelb, M.H., Leslie, C.C., (2008) "Regulation of cytosolic phospholipase A2alpha by hsp90 and a p54 kinase in okadaic acid-stimulated macrophages", J. Leukoc Biol., 84(3):798-806. reprint

Lambeau G., Gelb, M. H. (2008) "Biochmistry and physiology of mammalian secreted phospholipases A2" Annu Rev Biochem, 77:495-520.  reprint

Patel, MI, Singh, J, Niknami, M, Kurek, C, Yao, M, Lu, S, Maclean, F, King, NJ, Gelb, MH, Scott, KF, Russell, PJ, Boulas, J, Dong, Q. (2008) "Cytosolic Phospholipase A2-alpha:  A potential therapeutic target for prostrate cancer", Clin Cancer Res., 14:8070-8079.  reprint

Su, W, Yeku, O, Olepu, S, Genna, A, Park, J-S, Ren, H, Du, G, Gelb, MH, Morris, AJ, and Frohman, MA (2008) "FIPI, a Phospholipase D pharmacological inhibitor that alters cell spreading and inhibits chemotaxis," Molecular Pharmocology Fast Forward, published December 8, 2008, mol.108.053298. reprint

Oslund, RC, Cermak, N, Verlinde, CLM, Gelb, MH, (2008) "Simplified YM-26734 Inhibitors of secreted phospholipase A2 group IIA," Bioorganic and Medicinal Chemistry Letters, 18:5415-4319.  reprint  supp.

Wilensky, RL, Shi, Y, Mohler, ER, III, Hamamdzic, D, Burgert, ME, Li, J, Postle, A, Fenning, RS, Bollinger, JG, Hoffman, BE, Pelchovitz, DJ, Yang, J, Mirabile, RC, Webb, CL, Zhang, L, Zhang, P, Gelb, MH, Walker, MC, Zalewski, A, and Macphee, CH, (2008) "Inhibition of lipoprotein-associated phospholipase A2 reduces complex coronary atherosclerotic plaque development," Nature Medicine, 14 (10):1059-1066. reprint  supp.

Oslund, RC, Cermak, N, Gelb, MH, (2008) "Highly specific and broadly potent inhibitors of mammalian secreted phospholipases A2," J. Med. Chem., 51:4708-4714.  reprint.pdf  supp.

Tribler, L, Jensen, LT, Jørgensen, K, Brünner, N, Gelb, MH, Nielsen, HJ and Jensen, SS, (2007) “Increased expression and activity of group IIA and X secretory phospholipase A2 in Peritumoral versus central colon carcinoma tissue,” Anticancer Research, 27:3179-3186. reprint.pdf

Morgan, D, Cherny, VV, Finnegan A, Bollinger J, Gelb MH, DeCoursey TE. (2007) “Sustained activation of proton cheannels and NADPH oxidase in human eosinophils and murine granulocytes requires PKC but not CPLA2 alpha activity,” J. Physiol., 579(Pt 2):327-344.  reprint.pdf

Hallstrand, TS, Chi, EY, Singer, AG, Gelb, MH, Henderson, Jr., WR (2007) “Secreted phospholipase A2 group X overexpression in asthma and bronchial hyperresponsiveness,” Am. J. Respir Crit Care Med., 176:1072-1078.  reprint

Wichmann O, Gelb MH, Schultz, C. (2007) “Probing Phospholipase A2 with fluorescent phospholipid Substrates,” ChemBioChem, 8:1555-1569. reprint.pdf

Ghomashchi, F., Bollinger, J.G. and Gelb, M. H. (2007) "Facile preparation of leukotrienes C4, D4 and E4 containing carbon-13 and nitrogen-15 for quantification of cysteinyl leukotrienes by mass spectrometry," J. Radiolabel. Compds Radiopharm. 50:729 reprint.pdf

Ghosh, M., Loper, R., Ghomashchi, F., Tucker, D.E., Bonventre, J.V., Gelb, M.H. and Christina C. Leslie. (2007) "Function, Activity, and Membrane Targeting of Cytosolic Phospholipase A2-zeta in Mouse Lung Fibroblasts," J. Biol. Chem. 282:11676 reprint.pdf

Henderson, W.R., Chi, E.Y., Bollinger, J.G., Tien, Y.T., Ye, X., Castelli, L. Rubtsov, Y.P., Singer, A.G., Chiang, G.K., Nevalainen, T., Rudensky, A.Y. and M. Gelb. (2007) "Importance of Group X-secreted Phospholipase A2 in Allergen-Induced airway inflammation and remodeling in a mouse asthma model," J. Exp. Med. April 16  204:865-877 reprint.pdf

Rouault M, Calvez CL, Boilard E, Surrel F, Singer A, Ghomashchi F, Bezzine S, Scarzello S, Bollinger J, Gelb MH, Lambeau G. (2007) " Recombinant production and properties of binding of the full set of mouse secreted phospholipases a(2) to the mouse m-type receptor," Biochemistry  46:1647-62. reprint.pdf

Boilard, E., Rouault, M., Surrel, F., Le Calvez, C. Bezzine, S., Singer, A., Gelb, M.H. and Gerard Lambeau. (2006) "Secreted Phospholipase A2 Inhibitors Are Also Potent Blockers of Binding to the M-Type Receptor," Biochemistry  45:13202 reprint.pdf

Karabina, S.A., Brocheriou, I., Le Naour, G., Agrapart, M., Durand, H., Gelb, M.H., Lambeau, G. and Ewa Ninio.(2006) "Atherogenic properties of LDL particles modified by human group X secreted phospholipase A2 on human endothelial cell function," The FASEB Journal 20:E2  [Published online Oct. 31] reprint.pdf

Ghosh, M., Loper, R., Gelb, M.H. and Leslie, C.C.(2006) “Identification of the Expressed Form of Human Cytosolic Phospholipase A2β (cPLA2β),” J. Biol. Chem. 281:16615. reprint.pdf

Gelb, M. H., Mounier, Carine M.,  Hefner, Ying, Watson, Steve P., Platelet Phospholipases A2. In Platelets in thrombotic and non-thrombotic disorders: pathophysiology, pharmacology, and therapeutics, Gresele, P. e. a., Ed. Cambridge University Press: Cambridge, 2002; pp 221-237. reprint.pdf

Ni,Z., Okeley, N. M., Smart, B.P., and Michael H. Gelb (2006)" Intracellular Actions of Group IIA Secreted Phospholipase A2 and Group IVA Cytosolic Phospholipase A2 Contribute to Arachidonic Acid Release and Prostaglandin Production in Rat Gastric Mucosal Cells and Transfected Human Embryonic Kidney Cells" J. Biol. Chem., 281: 16245-55 . reprint.pdf

Eerola, L.I., Surrel, F., Nevalainen, T.J., Gelb, M.H., Lambeau, G. and V. Jukka O. Laine (2006) "Analysis of expression of secreted phospholipases A2 in mouse tissues at protein and mRNA levels," Biochimica et Biophysica Acta [Epub ahead of print]  April 10 reprint.pdf

Rouault, M., Rash, L. D., Escoubas, P., Boilard, E., Bollinger,J., Lomonte,B., Maurin, T.,  Guillaume,C., Canaan, S., Deregnaucourt, C., Schrevel,J., Doglio, A., Gutierrez, J. M., Lazdunski, M., Gelb, M. H., and G. Lambeau. (2006) "Neurotoxicity and Other Pharmacological Activities of the Snake Venom
Phospholipase A2 OS2: The N-Terminal Region Is More Important Than Enzymatic
Activity," Biochemistry  [Epub ahead of print]  Feb. 14 reprint.pdf

Smart, B.P., Oslund, R.C. and Walsh, L.A. (2006) " The First Potent Inhibitor of Mammalian Group
X Secreted Phospholipase A2: Elucidation of Sites for Enhanced Binding," J. Med. Chem. Feb. 8 ; [Epub ahead of print]  reprint.pdf supplement

Huhtinen, H.T., Gronroos, J.O., Gronroos, J.M. Uksila, J., Gelb, M.H., Nevalainen, T.J. and Laine, V.J. (2006) "Antibacterial effects of human group IIA and group XIIA phospholipase A2 against Helicobacter pylori in vitro," APMIS 114:127-30 reprint.pdf

Vandal, O.H., Gelb, M.H., Ehrt, S. and Nathan, C.F. (2006) "Cytosolic Phospholipase A2 enzymes are not required by mouse bone marrow-derived macrophages for the control of Mycobacterium tuberculosis in Vitro ,"  Infect. Immun. Mar. 74:1751-6. reprint.pdf

Nevalainen, T.J., Eerola, L.I., Rintala, E., Laine, V.J., Lambeau, G. and Gelb, M.H. (2005) " Time-resolved fluoroimmunoassays of the complete set of secreted phospholipases A2 in human serum," Biochim Biophys Acta. 1733:210-213  reprint.pdf

Forsell, P.K., Olsson, A.O., Andersson, E., Nallan, L. and Gelb, M.H. (2005) "Polychlorinated biphenyls induce arachidonic acid release in human platelets in a tamoxifen sensitive manner via activation of group IVA cytosolic phospholipase A(2)-alpha,"  Biochem Pharmacol. Nov. 12 ; [Epub ahead of print]  reprint.pdf

Petan, T., Krizaj, I., Gelb, MH. and Pungercar, J. (2005)  "Ammodytoxins, potent presynaptic neurotoxins, are also highly efficient phospholipase A(2) enzymes,"  Biochemistry,  44:12535-12545 reprint.pdf

Tucker, D.E., Stewart, A., Nallan, L., Bendale, P., Ghomashchi, F., Gelb, MH. and Leslie, CC.  (2005)

"Group IVC cytosolic phospholipase A2{gamma} is farnesylated and palmitoylated in mammalian cells,"
J. Lipid Res., 46:2122-33. reprint.pdf

 

Kita, Y., Takahashi, T., Uozumi, N., Nallan, L.,Gelb, M.H. and Shimizu, T. (2005) "Pathway-oriented profiling of lipid mediators in macrophages," Biochem. Biophys. Res. Commun., 330:898-906. reprint.pdf

Nielsen, R.D., Che, K., Gelb, M.H. and Robinson, B.H. (2005) "A Ruler for Determining the Position of Proteins in Membranes," J. Am. Chem. Soc., 127:6430-42. reprint.pdf   data supplement.pdf

Babon, A., Almunia, C., Boccaccio, C., Beaumelle, B., Gelb, M.H., Menez, A., Maillere, B., Abastado, J.P., Salcedo, M. and Gillet, D., (2005) "Cross-presentation of a CMV pp65 epitope by human dendritic cells using bee venom PLA(2) as a membrane-binding vector," FEBS Lett., 579:1658-64. reprint.pdf

Yuan, C., Tu, S., Gelb, M.H. and Tsai, M-D. (2005) "Unusual Four-Bond Secondary H/D Isotope Effect Supports a Short-Strong Hydrogen Bond between Phospholipase A2 and a Transition State Analogue Inhibitor," Biochemistry, 44:4748-54. reprint.pdf

Granata, F., Petraroli, A., Boilard, E., Bezzine, S., Bollinger, J., Del Vecchio, L., Gelb, M.H., Lambeau, G., Marone, G., and Triggiani M. (2005) "Activation of cytokine production by secreted phospholipase A2 in human lung macrophages expressing the m-type receptor," J. Immunol., 174: 464-74. reprint.pdf

Masuda, S., Murakami, M., Matsumoto, S., Eguchi, N., Urade, Y., Lambeau, G., Gelb, M.H., Ishikawa, Y., Ishii, T. and Kudo, I. (2004) "Localization of various secretory phospholipase A(2) enzymes in male reproductive organs," Biochim Biophys Acta., 1686:61-76. reprint.pdf

Ivanovski, G., Petan, T., Krizaj, I., Gelb, M.H., Gubensek, F. and Pungercar, J. (2004) "Basic amino acid residues in the beta-structure region contribute, but not critically, to presynaptic neurotoxicity of ammodytoxin A," Biochim Biophys Acta., 1702:217-25. reprint.pdf

Buhot, C., Chenal, A., Sanson, A., Pouvelle-Moratille, S., Gelb, M.H., Menez, A., Gillet, D. and Maillere, B. (2004) "Alteration of the tertiary structure of the major bee venom allergen Api m 1 by multiple mutations is concomitant with low IgE reactivity," Protein Sci. 13:2970-8. reprint.pdf

Smart, B.P., Pan, Y.H., Weeks, A.K., Bollinger, J.G., Bahnson, B.J. and Gelb, M.H. (2004) "Inhibition of the complete set of mammalian secreted phospholipases A(2) by indole analogues a structure-guided study," Bioorg Med Chem., 12:1737-49. reprint.pdf

Landreville, S., Coulombe, S., Carrier, P., Gelb, M.H., Guérin, S.L., and Salesse, C. (2004) Expression of Phospholipases A2 and C in Human Corneal Epitheliel Cells," IOVS, 45:3997-4003. reprint.pdf

Bollinger, J.G., Diraviyam, K., Ghomashchi, F., Murray, D., and Gelb, M.H. (2004) "Interfacial Binding of Bee Venom Secreted Phospholipase A2 to Membranes Occurs Predominantly by a Nonelectrostatic Mechanism," Biochemistry, 43:13293-13304. reprint.pdf

Rubin, B.B., Downey, G.P., Koh, A., Degousee, N., Ghomashchi, F., Nallan, L., Stefanski, E., Smart, B.P., Lindsay, T.F., Cherepanov, V., Vachon, E., Kelvin, D., Sadilek, M., Brown, G.E., Yaffe, M.B., Plumb, J., Grinstein, S., Glogauer, M., and Gelb, M.H. (2004) "Cytosolic phospholipase A2 alpha is necessary for platelet activating factor biosynthesis, efficient neutrophil mediated bacterial killing and the innate immune response to pulmonary infection," J. Biol. Chem., 280:7519-29. reprint.pdf

Haas, U., Podda, M., Behne, M., Gurrieri, S., Alonso, A., Fürstenberger, G., Pfeilschifter, J., Lambeau, G., Gelb, M.H., and Kaszkin, M. (2004) "Characterization and Differentiation-dependent Regulation of Secreted Phospholipases A2 in Human Keratinocytes and in Healthy and Psoriatic Human Skin," J. Invest. Dermatol., 124:204-211. reprint.pdf

Sved, P., Scott, K.F., McLeod, D., King, N.J., Singh, J., Tsatralis, T., Nikolov, B., Boulas, J., Nallan, L., Gelb, M.H., Sajinovic, M., Graham, G.G., Russell, P.J., and Dong, Q. (2004) "Oncogenic action of secreted phospholipase A2 in prostate cancer," Cancer Res., 64: 6934-40. reprint.pdf

Nielsen, R.D., Canaan, S., Gladden, J.A., Gelb, M.H., Mailer, C., and Robinson, B.H. (2004) "Comparing continuous wave progressive saturation EPR and time domain saturation recovery EPR over the entire motional range of nitroxide spin labels," J Magn Reson., 169:129-63. reprint.pdf

Leslie, C.C., Gelb, M.H. (2004) "Assaying phospholipase A2 activity," Methods Mol Biol., 284:229-42. reprint.pdf

Mounier, C.M., Ghomashchi, F., Lindsay, M.R., James, S., Singer, A.G., Parton, R.G., and Gelb, M.H. (2004) "Arachidonic acid release from mammalian cells transfected with human groups IIA and X secreted phospholipase A2 occurs predominantly during the secretory process and with the involvement of cytosolic phospholipase A2-a," J. Biol. Chem. 279:25024-25038. reprint.pdf, supplement

Okeley, N.M, Gelb, M.H. (2004) "Membrane Transport, Structure, Function, and Biogenesis: A designed probe for acidic phospholipids reveals the enriched anionic character of the cytosolic face of the mammalian plasma membrane," J. Biol. Chem, 279:21833-21840. reprint.pdf

Stephane Canaan, S., Zoltan Zadori, Z., Ghomashchi,F., Bollinger, J., Sadilek, M., Marie Eve Moreau, M.E., Tijssen, P., and Gelb, M.H. (2004) "Interfacial enzymology of parvovirus phospholipases A2," J. Biol. Chem, 279:14502-14508. reprint.pdf

Triggiani, M., Giannattasio, G., Balestrieri, B., Granata, F., Gelb, M.H., de Paulis, A., and Marone, G. (2004) "Differential modulation of mediator release from human basophils and mast cells by mizolastine," Clin. Exp. Allergy, 34:241-9. reprint.pdf

Rounault, M., Bollinger, J.G., Lazdunski, M., Gelb, M.H., Lambeau, G. (2003) "Novel Mammalian Group XII Secreted Phospholipase A2 Lacking Enzymatic Activity," Biochemistry, 42:11494-11503. reprint.pdf

Beck, S., Lambeau, G., Scholz-Pedretti, K., Gelb, M.H., Janssen, M.J.W., Edwards, S.H., Wilton, D.C., Pfeilschifter, J., and Kaszkin, M. (2003) "Potentiation of TNFa-induced sPLA2-IIA expression in mesangial cells by an autocrine loop involving secreted phospholipase A2 and PPARa activation," J. Biol. Chem., 278:29799-29812. reprint.pdf

Gurrieri, S., Fürstenberger, G., Schadow, A., Haas, U., Singer, A.G., Ghomashchi, F., Pfeilschifter, J., Lambeau, G., Gelb, M.H., and Kaszkin, M. (2003) "Differentiation-dependent regulation of secreted phospholipases A2 in murine epidermis," J. Invest. Dermatol., 121:156-164. reprint.pdf

Poi, M.J., Tomaszewski, J.W., Yuan, C., Dunlap, C.A., Andersen, N.H., Gelb, M.H., and Tsai, M.-D. (2003) "A low-barrier hydrogen bond between histidine of secreted phospholipase A2 and a transition state analog inhibitor," J. Mol. Biol., 329:997-1009. reprint.pdf

Beers, S.A., Buckland, A.G., Giles, N., Gelb, M.H. and Wilton, D.C. (2003) "Effect of tryptophan insertions on the properties of the human group IIA phospholipase A2: mutagenesis produces an enzyme with characteristics similar to those of the human group V phospholipase A2," Biochem., 42(24):7326-7338. reprint.pdf

Murakami, M., Masuda, S., Shimbara, S., Bezzine, S., Lazdunski, M., Lambeau, G., Gelb, M.H., Matsukura, S., Kokubu, F., Adachi, M. and Kudo, I. (2003) "Cellular Arachidonate-Releasing Function of Novel Classes of Secretory Phospholipase A2s (Group III and XII)," J. Biol. Chem., 278:10657-10667. reprint.pdf

Singer, A.G., Ghomashchi, F., Le Calvez, C., Bollinger, J., Bezzine, S., Rouault, M., Sadilek, M., Lazdunski, M., Lambeau, G., and Gelb, M.H. (2002) "Interfacial Kinetic and Binding Properties of the Complete set of Human and Mouse Groups I, II, V, X, and XII Secreted Phospholipases A2," J. Biol. Chem., 277:48535-48549. reprint.pdf

Bezzine, S., Bollinger, J.G., Singer, A.G., Veatch, S.L., Keller, S.L., and Gelb, M.H. (2002) "On the Binding Preference of Human Groups IIA and X Phospholipases A2 for Membranes with Anionic Phospholipids," J. Biol. Chem., 277:48523-48534. reprint.pdf

Koprivnjak, T., Peschel, A., Gelb, M.H., Liang, N.S., and Weiss, J.O. (2002) "Role of charge properties of bacterial envelope in bactericidal action of human Group IIA phospholipase A2 against Staphylococcus andreas," J. Biol. Chem., 277:47636-47644. reprint.pdf

Murakami, M., Yoshihara, K., Shimbara, S., Lambeau, G., Gelb, M.H., Singer, A.G., Sawada, M., Inagaki, N., Nagai, H., Ishihara, M., Ishikawa, Y., Ishii, T., and Kudo, I. (2002) "Cellular Arachidonate-releasing Function and Inflammation-associated Expression of Group IIF Secretory Phospholipase A2," J. Biol. Chem., 277(21):19145-19155. reprint.pdf

Pan, Y.H., Yu, B.-Z., Singer, A.G., Ghomashchi, F., Lambeau, G., Gelb, M.H., Jain, M.J., and Bahnson, B.J. (2002) "Crystal structure of human group-X secreted phospholipase A2; electrostatically neutral interfacial binding surface targets zwitterionic membranes," J. Biol. Chem., 277:29086-29093. reprint.pdf

Canaan, S., Nielsen, R., Ghomashchi, F., Robinson, B.H., and Gelb, M.H. (2002) "Unusal Mode of Binding of human group-IIA secreted phospholipase A2 to anionic interfaces as studied by continuous wave and time domain electron paramagnetic resonance spectroscopy," J. Biol. Chem, 277:30984-30990. reprint.pdf

Murakami, M., Yoshihara, K., Shimbara, S., Lambeau, G., Singer, A., Gelb, M.H., Sawada, M., Inagaki, N., Nagai, H., and Kudo, I. (2002) "Arachidonate release and eicosanoid generation by group IIE phospholipase A2," Biochem. Biophys. Res. Comm., 292(3):689-696. reprint.pdf

Degousee, N., Ghomashchi, F., Stefanski, E., Singer, A., Smart, B.P., Borregaard, N., Reithmeier, R., Lindsay, T.F., Lichtenberger, C., Reinisch, W., Lambeau, G., Arm, J., Tischfield, J.T., Gelb, M.H., and Rubin, B.B. (2001) "Groups IV, V and X Phospholipases A2 in Human Neutrophils," J. Biol. Chem., 277(7):5061-5073. reprint.pdf

Beers, S.A., Buckland, A.G., Koduri, R.S., Cho, W., Gelb, M.H., and Wilton, D.C. (2002) "The antibacterial properties of secreted phospholipases A2: a major physiological role for the group IIA enzyme that depends on the very high pI of the enzyme to allow penetration of the bacterial cell wall," J. Biol. Chem., 277(3):1788-1793. reprint.pdf

Koduri, R.S., Grönroos, J.O., Laine, V.J.O., Calvez, C.L., Lambeau, G., Nevalainen, T.J., and Gelb, M.H. (2002) "Bactericidal Properties of Human and Murine Groups I, II, V, X, and XII Secreted Phospholipases A2," J. Biol. Chem., 277:5849-5857. reprint.pdf

Berg, O.G., Gelb, M.H., Tsai, M.-D., and Jain, M.K. (2001) "Interfacial Enzymology: The Secreted Phospholipase A2-Paradigm," Chem. Rev., 101:2613-2653. reprint.pdf

Muthalif, M.M., Hefner, Y., Canaan, S., Harper, J., Zhou, H., Parmentier, J.-H., Aebersold, R., Gelb, M.H., and Malik, K.U. (2001) "Functional Interacton of Calcium/Calmodulin-Dependent Protein Kinase II and Cytosolic Phospholipase A2." J. Biol. Chem., 276: 39653-39660. reprint.pdf

Ghomaschi, F., Stewart, A., Hefner, Y., Ramanadham, S., Turk, J., Leslie, C.C., and Gelb, M.H. (2001) "A pyrrolidine-based specific inhibitor of cytosolic phospholipase A2[alpha] blocks arachidonic acid release in a variety of mammalian cells." Biochimica et Biophysica Acta. 78118:1-7. reprint.pdf

Min, J.H., Wilder, C., Aoki, J., Arai, H., Inoue, K., Paul, L., and Gelb, M.H. (2001) "Platelet-Activating Factor Acetylhydrolases: Broad Substrate Specificity and Lipoprotein Binding Does Not Modulate the Catalytic Properties of the Plasma Enzyme." Biochemistry. 40(15):4539-4549. reprint.pdf

Schadow, A., Scholz-Pedretti, K., Lambeau, G., Gelb, M.H., Furstenberger, G., Pfeilschifter, J., and Kaszkin, M. (2001) "Characterization of group X phospholipase A2 as the major enzyme secreted by human keratinocytes and its regulation by the phorbol ester TPA," J. Investig. Dermatol., 116(1):31-39. reprint.pdf

Valentin E, Singer AG, Ghomashchi F, Lazdunski M, Gelb MH, Lambeau G. Cloning and Recombinant Expression of Human Group IIF-Secreted Phospholipase A(2). Biochem Biophys Res Commun. 279:223-228. reprint.pdf

Mounier CM, Luchetta P, Lecut C, Koduri RS, Faure G, Lambeau G, Valentin E, Singer A, Ghomashchi F, Beguin S, Gelb MH, Bon C. Related Articles, Links Basic residues of human group IIA phospholipase A2 are important for binding to factor Xa and prothrombinase inhibition comparison with other mammalian secreted phospholipases A2. Eur J Biochem. 2000 Aug;267(16):4960-4969. reprint.pdf

M. Murakami, R. S. Koduri, A. Enomoto, S. Shimbara, M. Seki, K. Yoshihara, A. Singer, E. Valentin, F. Ghomashchi, G. Lambeau, M.H. Gelb, and I. Kudo (2000) Distinct Arachidonate-Releasing Functions of Mammalian Secreted Phospholipase A2s in Human Embryonic Kidney 293 and Rat Mastocytoma RBL-2H3 Cells through Heparin Sulfate Shuttling and External Plasma Membrane Mechanisms, J. Biol. Chem., 276(13):10083-10096. reprint.pdf

Ying Hefner, Angelika G. Borsch-Haubold, Makomoto Murakami, Jonathan I. Wilde, Sophie Pasquet, David Schieltz, Farideh Ghomashchi, John R. Yates, III,Christopher G. Armstrong, Andrew Paterson, Philip Cohen, Rikiro Fukunaga, Tony Hunter, Ichiro Kudo, Steve P. Watson, and Michael H. Gelb. Serine-727 phosphorylation and activation of cytosolic phospholipase A2 by MNK1-related protein kinases J. Biol. Chem., 275:37543-37551 reprint.pdf

Enomoto A., Murakami, M., Valentin, E., Lambeau, G. Gelb, M. H. and Kudo, I; Redundant and Segregated Functions of Granule-Associated Heparin-Binding Group II Subfamily of Secretory Phospholipases A2 in the Regulation of Degranulation and Prostaglandin D2 Synthesis in Mast Cells: J. Immunol. 4007-4014 (2000). reprint.pdf

Gelb MH, Min J, Jain MK. (2000) Do membrane-bound enzymes access their substrates from the membrane or aqueous phase: interfacial versus non-interfacial enzymes. Biochim Biophys Acta. 2000 Oct 31; 1488(1-2):20-27. reprint.pdf

Linda S. Jung, Jennifer S. Shumaker-Parry, Charles T. Campbell,* Sinclair S. Yee, and Michael H. Gelb* ; Quantification of Tight Binding to Surface-Immobilized Phospholipid Vesicles Using Surface Plasmon Resonance: Binding Constant of Phospholipase A2, Journal of the American Chemical Society; 2000; 122(17); 4177-4184. reprint.pdf

Gelb MH, Valentin E, Ghomashchi F, Lazdunski M, Lambeau G. Cloning and recombinant expression of a structurally novel human secreted phospholipase A2. J Biol Chem. 2000; 275(51); 39823-39826. reprint.pdf

Bezzine S, Koduri RS, Valentin E, Murakami M, Kudo I, Ghomashchi F, Sadilek M, Lambeau G, Gelb MH. Exogenously added human group X secreted phospholipase A(2) but not the group IB, IIA, and V enzymes efficiently release arachidonic acid from adherent mammalian cells. Journal of Biological Chemistry. 275(5): 3179-91, Feb 2000 reprint.pdf

Valentin E, Ghomashchi F, Gelb MH, Lazdunski M, Lambeau G. Novel secreted phospholipase A(2) with homology to the group III bee venom enzyme. Journal of Biological Chemistry. 275, 7492-7496, 2000 reprint.pdf

Valentin E, Ghomashchi F, Gelb MH, Lazdunski M, Lambeau G. On the diversity of secreted phospholipases A(2). Cloning,tissue distribution, and functional expression of two novel mouse group II enzymes. Journal of Biological Chemistry. 274(44):31195-202, Oct 1999 reprint.pdf

F. Ghomashchi, R. Loo, J. Balsinde, F. Bartoli, E. A. Dennis, and M. H. Gelb (1999) Trifluoromethyl ketones and methyl fluorophosphonates as inhibitors of group IV and VI Phospholipases A2: structure-function studies with vesicle, micelle and membrane assays. Biochim. Biophys. Acta 1420, 45-56. reprint.pdf

Min JH, Jain MK, Wilder C, Paul L, Apitz-Castro R, Aspleaf DC, Gelb MH. Membrane-bound plasma platelet activating factor acetylhydrolase acts on substrate in the aqueous phase. Biochemistry. 38(39): 12935-42, Sep 1999. reprint.pdf

Buschbeck M, Ghomashchi F, Gelb MH, Watson SP, Borsch-Haubold AG. Stress stimuli increase calcium-induced arachidonic acid release through phosphorylation of cytosolic phospholipase A2. Biochemical Journal. 344 Pt 2: 359-66, Dec 1999. reprint.pdf

Valentin E, Koduri RS, Scimeca JC, Carle G, Gelb MH, Lazdunski M, Lambeau G. Cloning and recombinant expression of a novel mouse-secreted phospholipase A2. J Biol Chem. 1999 Jul 2;274(27):19152-60. reprint.pdf

Gelb, MH, Cho, W, and Wilton, DC. (1999) "Interfacial binding of secreted phospholipases A2: more than electrostatics and a major role for tryptophan," Curr. Opinion in Struct. Biol., 9(4):428-432. reprint.pdf

Han SK, Kim KP, Koduri R, Bittova L, Munoz NM, Leff AR, Wilton DC, Gelb MH, Cho W. Roles of Trp31 in high membrane binding and proinflammatory activity of human group V phospholipase A2. J Biol Chem 274(17): 11881-8, Apr 1999. reprint.pdf

Ball A; Nielsen R; Gelb MH; Robinson BH. Interfacial membrane docking of cytosolic phospholipase A2 C2 domain using electrostatic potential-modulated spin relaxation magnetic resonance.. Proc Natl Acad Sci U S A. 96(12): 6637-42, 8 Jun 1999. reprint.pdf

Hixon MS, Ball A, Gelb MH. Calcium-dependent and -independent interfacial binding and catalysis of cytosolic group IV phospholipase A2. Biochemistry. 1998 Jun 9;37(23):8516-26. reprint.pdf

Ghomashchi F;Lin Y;Hixon M S;Yu B Z;Annand R;Jain M K;Gelb M H. Interfacial recognition by bee venom phospholipase A2: insights into nonelectrostatic molecular determinants by charge reversal mutagenesis.. Biochemistry. 37(19): 6697-710, 12 May 1998. reprint.pdf

Lin Y; Nielsen R;Murray D;Hubbell W L;Mailer C;Robinson B H;Gelb M H. Docking phospholipase A2 on membranes using electrostatic potential-modulated spin relaxation magnetic resonance. Science. 279(5358): 1925-9, 20 Mar 1998 reprint.pdf

Koduri RS, Baker SF, Snitko Y, Han SK, Cho W, Wilton DC, Gelb MH. Action of human group IIa secreted phospholipase A2 on cell membranes. Vesicle but not heparinoid binding determines rate of fatty acid release by exogenously added enzyme. J Biol Chem. 273(48): 32142-53, Nov 1998. reprint.pdf

Borsch-Haubold AG, Bartoli F, Asselin J, Dudler T, Kramer RM, Apitz-Castro R, Watson SP, Gelb MH. Identification of the phosphorylation sites of cytosolic phospholipase A2 in agonist-stimulated human platelets and HeLa cells. J Biol Chem. 1998 Feb 20;273(8):4449-58. reprint.pdf

Nicolas JP, Lin Y, Lambeau G, Ghomashchi F, Lazdunski M, Gelb MH. Localization of structural elements of bee venom phospholipase A2 involved in N-type receptor binding and neurotoxicity. J Biol Chem. 1997 Mar 14;272(11):7173-81. reprint.pdf

Snitko Y, Koduri RS, Han SK, Othman R, Baker SF, Molini BJ, Wilton DC, Gelb MH, Cho W. Mapping the interfacial binding surface of human secretory group IIa phospholipase A2. Biochemistry. 1997 Nov 25;36(47):14325-33. reprint.pdf

Yu B Z;Ghomashchi F;Cajal Y;Annand R R;Berg O G;Gelb M H;Jain M K. Use of an imperfect neutral diluent and outer vesicle layer scooting mode hydrolysis to analyze the interfacial kinetics, inhibition, and substrate preferences of bee venom phospholipase A2. Biochemistry. 36(13): 3870-81, 1 Apr 1997. reprint.pdf

Annand RR, Kontoyianni M, Penzotti JE, Dudler T, Lybrand TP, Gelb MH. Active site of bee venom phospholipase A2: the role of histidine-34, aspartate-64 and tyrosine-87. Biochemistry. 1996 Apr 9;35(14):4591-601. reprint.pdf

de Carvalho MG, McCormack AL, Olson E, Ghomashchi F, Gelb MH, Yates JR 3rd, Leslie CC. Identification of phosphorylation sites of human 85-kDa cytosolic phospholipase A2 expressed in insect cells and present in human monocytes. J Biol Chem. 1996 Mar 22;271(12):6987-97. reprint.pdf

Bayburt T;Gelb M H. Interfacial catalysis by human 85 kDa cytosolic phospholipase A2 on anionic vesicles in the scooting mode. Biochemistry. 36(11): 3216-31, 18 Mar 1997. reprint.pdf

 

Protein Prenylation Reprints   back to top

Yun, B., Lee, H., Ghosh, M., Cravatt, B. F., Hsu, K. L., Bonventre, J. V., Ewing, H., Gelb, M. H., Leslie, C. C. (2013) "Serine hydrolase Inhibitors block necrotic cell death by preventing calcium overload of the mitochondria and permeability transition pore formation," J. Biol Chem., 289:1491-1504, PMID: 24297180 reprint

Fletcher, S., Keaney, E. P., Cummings, C. G., Blaskovich, M. A., Hast, M. A., Glenn, M. P., Chang, S.-Y., Bucher, C. J., Floyd, R. J., Katt, W. P., Gelb, M. H., Van Voorhis, W. C., Beese, L. S., Sebti, S. M., Hamilton, A. D. (2010) "Structure-based design and synthesis of potent, ethylenediamine-based, mammalian farnesyltransferase inhibitors as anticancer agents," J. Med. Chem., 53:6867-6888.  PMID:  20822181   reprint

Hast, M. A., Fletcher, S., Cummings, C.G., Pusateri, E.E., Blaskovich, M.A., Rivas, K., Gelb, M.H., Van Voorhis, W.C., Sebti, S.M., Hamilton, A.D., and Beese, L.S. (2009) "Structural basis for binding and selectivity of antimalarial and anticancer ethylenediamine inhibitors to protein farnesyltransferase", Chemistry & Biology, 16:181-192.  PMID:  19246009   reprint

Fletcher, S., Cummings, C.G., Rivas, K., Katt, W.P., Horney, C., Buckner, F.S., Chakrabarti, D., Sebti, S.M., Gelb, M.H., Van Voorhis, W.C., Hamilton, A.D. (2008) "Potent, Plasmodium-selective farnesyltransferase inhibitors that arrest the growth of malaria parasites:  Structure-activity relationships of ethylenediamine-analogue scaffolds and homology model validation", J. Med. Chem, 51(17):5176-5197. reprint  supp.

Bulbule, V. J., Rivas, K, Verlinde, C. L., Van Voorhis, W. C., Gelb, M. H., (2008) "2-oxotetrahydroquinoline-based antimalarials with high potency and metabolic stability," J. Med. Chem., 51:384-387. reprint.pdf  supp.

Olepu, S, Suryadevara, P. K., Rivas, K., Yokoyama, K., Verlinde, C. L., Chakrabarti, D., Van Voorhis, W. C., Gelb, M. H., (2008) “2-Oxo-tetrahydro-1,8-naphthyridines as selective inhibitors of malarial protein farnesyltransferase and as anti-malarials,” Bioorg Med Chem Lett., 18:494-497. reprint.pdf   supp.

Yokoyama, K., Gillespie, J. R., Van Voorhis, W. C., Buckner, F. S., Gelb, M. H., (2008) “Protein geranylgeranyltransferase-I of Trypanosoma cruzi,” Mol Biochem Parasitol, 157:32-43. reprint.pdf

Gillespie, J. R., Yokoyama, K, Lu, K, Eastman, R. T., Bollinger, J. G., Van Voorhis, W. C., Gelb, M. H., Buckner, F. S. (2007) “C-terminal proteolysis of prenylated proteins in trypanosomatids and RNA interference of enzymes required for the post-translational processing pathway of farnesylated proteins,” Mol. Biochem. Parasitol., 153:115-124. reprint.pdf

Gelb, MH (2007) “Drug discovery for malaria: a very challenging and timely endeavor,” Current Opinion in Chemical Biology, 11:440-445. reprint.pdf

Van Voorhis, W. C., Rivas, K. L., Bendale, P., Nallan, L., Horney, C., Barrett, L. K., Bauer, K. D., Smart, B. P., Ankala, S., Hucke, O., Verlinde, C. L., Chakrabarti, D., Strickland, C., Yokoyama, K., Buckner, F. S., Hamilton, A. D., Williams, D. K., Lombardo, L. J., Floyd, D., Gelb, M. H. (2007) “Efficacy, pharmacokinetics, and metabolism of tetrahydroquinoline inhibitors of plasmodium falciparum protein farnesyltransferase,” Antimicrob. Agents Chemother., 10:3659-3671. reprint.pdf  supp.

Bendale, P., Olepu, S., Suryadevara, P. K., Bulbule, V., Rivas, K., Nallan, L., Smart, B., Yokoyama, K., Ankala, S., Pendyala, P. R., Floyd, D., Lombardo, L. J., Williams, D. K., Buckner, F. S., Chakrabarti, D., Verlinde, C. L., Van Voorhis, W. C., Gelb, M. H. (2007) “Second generation tetrahydroquinoline-based protein farnesyltransferase inhibitors as antimalarials,” J. Med. Chem., 50:4585-4605. reprint.pdf  supp.

Gelb, M. H., Brunsveld, L., Hrycyna, C. A., Michaelis, S., Tamanoi, F., Van Voorhis, W. C. and Waldmann, H. (2006) "Therapeutic intervention based on protein prenylation and associated modifications," Nature Chem. Biol. 10: 518-528   reprint.pdf

Eastman, R. T., Buckner, F. S., Yokoyana, K., Gelb, M. H. and Van Voorhis, W .C. (2006) "Thematic review series: lipid posttranslational modifications. Fighting parasitic disease by blocking protein farnesylation," J. Lipid Res. 47: 233-40   reprint.pdf

Buckner, F. S., Eastman, R. T., Yokoyama, K.., Gelb, M. H., Van Voorhis, W. C. (2005) " Protein farnesyl transferase inhibitors for the treatment of malaria and African trypanosomiasis," Curr Opin Investig  Drugs 6:791-7 reprint.pdf

Yang, S. H., Bergo, M. O., Toth, J. I., Qiao, X., Hu, Y., Sandoval, S., Meta, M., Bendale, P., Gelb, M. H., Young, S. G., Fong, L. G. (2005) "Blocking protein farnesyltransferase improves nuclear blebbing in mouse fibroblasts with a targeted Hutchinson-Bilford progeria syndrome mutation," Prof. Natl. Acad. Sci  102:10291-6 reprint.pdf

Mallampalli, M .P., Huyer, G., Bendale, P., Gelb, M. H. and Michaelis, S. (2005) "Inhibiting farnesylation reverses the nuclear morphology defect in a HeLa cell model for Hutchinson-Gilford progeria syndrome," Proc. Natl. Acad. Sci. 102:14416-21 reprint.pdf

Toth, J. I., Yang, S. H., Qiao, X., Beigneux, A. P., Gelb, M. H., Moulson, C. L., Miner, J. H., Young, S. G. and  Fong, L. G. (2005) "Blocking protein farnesyltransferase improves nuclear shape in fibroblasts from humans with progeroid syndromes," Proc. Natl. Acad. Sci. 102:12873-8 reprint.pdf

Hucke, O., Gelb, M. H.., Verlinde, C. L. M. J. et al. (2005) "The protein farnesyltransferase inhibitor tipifarnib as a new lead for the development of drugs against Chagas disease,"  J. Med. Chem., 48: 5415-5418. reprint.pdf

Glenn, M. R., Chang, S. Y., Hucke, O., et al. (2005) "Structurally simple farnesyltransferase inhibitors arrest the growth of malaria parasites," Angew. Chem. Int. Ed.  41: 4903-4906. reprint.pdf

Toth, J. I., Yang, S. H., Qiao, X., et al.  (2005)  "Blocking protein farnesyltransferase improves nuclear shape in fibroblasts from humans with progeroid syndromes," Proc. Natl. Acad. Sci. 102:12873-12878. reprint.pdf

Eastman, R. T., White, J., Hucke, O., et al. (2005) "Resistance to a protein farnesyltransferase inhibitor in Plasmodium falciparum," J. Biol. Chem. 280:13554-13559. reprint.pdf

 

Yang, S. H., Bergo, M. O., Toth, J. L., Qiao, X., Hu, Y., Sandoval, S., Meta, M., Bendale, P., Gelb, M. H., Young, S. G. and Fong, L. G. (2005) "Blocking protein farnesyltransferase improves nuclear blebbing in mouse fibroblasts with a targeted Hutchinson-Gilford progeria syndrome mutation," Proc. Natl. Acad. Sci. 109:10291-10296. reprint.pdf

Nallan, L., Bauer, K. D., Bendale, P., Rivas, K., Yokoyama, K., Horney, C. P., Pendyala, P. R., Floyd, D., Lombardo, L. J., Williams, D. K., Hamilton, A., Sebti, S., Windsor, W. T., Weber, P. C., Buckner, F. S., Chakrabarti, D., Gelb, M. H. and Van Voorhis, W. C. (2005) "Protein farnesyltransferase inhibitors exhibit potent antimalarial activity," J. Med. Chem., 48:3704-3713. reprint.pdf   data supplement.pdf

Carrico, D., Ohkanda, J., Kendrick, H., Yokoyama, K., Blaskovich, M. A., Bucher, C. J., Buckner, F. S., Van Voorhis, W. C., Chakrabarti, D., Croft, S. L., Gelb, M. H., Sebti, S. M. and Hamilton, A. D. (2004) "In vitro and in vivo antimalarial activity of peptidomimetic protein farnesyltransferase inhibitors with improved membrane permeability," Bioorg Med Chem., 12:6517-26. reprint.pdf

Ohkanda, J., Buckner, F. S, Lockman, J. W., Yokoyama, K., Carrico, D., Eastman, R., Luca-Fradley, K., Davies, W., Croft, S. L., Van Voorhis, W. C., Gelb, M. H., Sebti, S. M., and Hamilton, A. D. (2004) "Design and Sythesis of Peptidominetic Protein Farnesyltransferase Inhibitors as Anti-Typanosoma brucei Agents,"J. Med. Chem., 47:432-445. reprint.pdf

Buckner, F., Yokoyama, K., Lockman, J., Aikenhead, K., Ohkanda, J., Sadilek, M., Sebti, S., Van Voorhis, W., Hamilton, A., and Gelb, M. H. (2003) "A class of sterol 14-demethylase inhibitors as anti-Trypanosoma cruzi agents," PNAS, 100:15149-15153. reprint.pdf

Ferri, N., Yokoyama, K., Sadilek, M., Paoletti, R., Apitz-Castro, R., Gelb, M. H., and Corsini, A. (2003) "Ajoene, a garlic compound, inhibits protein prenylation and arterial smooth muscle cell proliferation," Br. J. Pharmacol., 138:811-818. reprint.pdf

Clerici, F., Gelmi, M.L., Yokoyama, K., Pocar, D., Van Voorhis, W. C., Buckner, F. S., and Gelb, M. H. (2002) "Isothiazole dioxides: synthesis and inhibition of Trypanosoma brucei protein farnesyltrasnferase," Bioorg. Med. Chem. Lett., 12:2217-2220 reprint.pdf

Buckner, F. S., Eastman, R. T., Nepomuceno-Silva, J. L., Speelmon, E. C., Myler, P. J., Van Voorhis, W. C., and Yokoyama, K. (2002) "Cloning, heterologous expression, and substrate specificities of protein farnesyltransferases from Trypanosoma cruzi and Leishmania major," Molec. & Biochem. Parasitol., 122:181-188. reprint.pdf

Ferri, N., Arnaboldi, A., Orlandi, A., Yokoyama, K., Gree, R., Granata, A., Hachem, A., Paoletti, R., Gelb, M. H., and Corsini, A. (2001) "Effect of S(-) perillic acid on protein prenylation and arterial smooth muscle cell proliferation," Biochem. Pharm., 62:1637-1645. reprint.pdf

Nepomuceno-Silva, J. L., Yokoyama, K., de Mello, L. D. B., Mendonca, S. M., Paixao, J. C., Baron, R., Faye, J.-C., Buckner, F. S., Van Voorhis, W. C., Gelb, M. H., and Lopes, U. G. (2001) "TcRho1, a farnesylated Rho family homologue from Trypanosoma cruzi," J. Biol. Chem., 276:29711-29718. reprint.pdf

Ohkanda, J., Lockman, J. W., Yokoyama, K., Gelb, M. H., Croft, S., Kendrick, H., Harrell, M. I., Feagin, J. E., Blaskovich, M. A., Sebti, S. M., and Hamilton, A. D. (2001) "Peptidomimetic inhibitors of protein farnesyltransferase show potent antimalarial activity," Bioorg. Med. Chem. Lett., 11:761-764. reprint.pdf

Terry A. Cook, Ghomashchi, F., Gelb, M. H., Florio, S. K., and Beavo, J. A. (2000) The delta subunit of type 6 phosphodiesterase reduces light-induced cGMP hydrolysis in rod outer segments J. Biol. Chem., 276:5248-5255. reprint.pdf

Cook, T. A., Ghomashchi, F., Gelb, M. H., Florio, S., Beavo, J. A. (2001) "Binding of the delta subunit to rod phosphodiesterase catalytic subunits requires methylated, prenylated C-termini of their catalytic subunits," Biochemistry 2000. reprint.pdf

Buckner, F. S., Yokoyama, K., Nguyen, L., Grewal, A., Erdjument-Bromage, H., Tempst, P., Strickland, C. L., Xiao, L, Van Voorhis, W. C., Gelb, M. H. (2000) "Cloning, heterologous expression, and distinct substrate specificity of protein farnesyltransferase from Trypanosoma brucei," J Biol Chem. 2000 Jul 21;275(29):21870-6. reprint.pdf

Yokoyama, K., Trobridge, P., Buckner, F. S., Van Voorhis, W. C., Stuart. K. D., Gelb, M. H. (1998) "Protein farnesyltransferase from Trypanosoma brucei. A heterodimer of 61- and 65-kda subunits as a new target for antiparasite therapeutics," Journal of Biological Chemistry. 273(41): 26497-505, Oct 1998. reprint.pdf

Jang, G. F. and Gelb, M. H. (1998) "Substrate specificity of mammalian prenyl protein-specific endoprotease activity," Biochemistry. 37(13): 4473-81, reprint.pdf

Yokoyama, K., Trobridge, P., Buckner, F. S., Scholten, J., Stuart, K. D., Van Voorhis, W. C., and Gelb, M. H. (1998) "The effects of protein farnesyltransferase inhibitors on trypanosomatids: inhibition of protein farnesylation and cell growth." Molecular and Biochemical Parasitology, 94:87-97. reprint.pdf

Yokoyama, K., Lin, Y., Stuart, K. D., and Gelb, M .H. (1997) "Prenylation of proteins in Trypanosoma brucei." Molecular & Biochemical Parasitology, 87:61-69. reprint.pdf

Yokoyama, K., Zimmerman, K., Scholten, J. and Gelb M. H. (1997) "Differential prenyl pyrophosphate binding to mammalian protein geranylgeranyltransferase-I and protein farnesyltransferase and its consequence on the specificity of protein prenylation,"

J Biol Chem 272: 3944-52 reprint.pdf

Dudler T. and Gelb M. H. (1996) "Palmitoylation of Ha-Ras facilitates membrane binding, activation of downstream effectors, and meiotic maturation in Xenopus oocytes,"

J Biol Chem. 271:11541-7. reprint.pdf

McGeady, P., Kuroda, S., Shimizu, K., Takai, Y., Gelb, M. H. (1995) "The farnesyl group of H-Ras facilitates the activation of a soluble upstream activator of mitogen-activated protein kinase,"

J Biol Chem. 270: 26347-51 reprint.pdf
 
 

Anti-Parasite Drug Design (other than protein prenylation) Reprints   back to top

Tatipaka, H. B., Gillespie, J. R., Chatterjee, A. K., Norcross, N. R., Halverson, M. A., Ranade, R. M., Nagendar, P., Creason, S. A., McQueen, J., Duster, N. A., Nagle, A., Supek, F., Molteni, V. Wenzler, T., Brun, R., Glynne, R., Buckner, F. S., and Gelb, M. H. (2014) "Substituted 2-Phenylimidazopyridines:  A new class of drug leads for human African Trypanosomiasis", J. Med. Chem., 57(3):828-835, PMID: 24354316, PMCID:  3962778.. reprint

Buckner F. S., Fau - Bahia, M. T., Bahia Mt Fau - Suryadevara, P. K., Suryadevara Pk Fau - White, K. L., White Kl Fau - Shackleford, D. M., Shackleford Dm Fau - Chennamaneni, N. K., Chennamaneni Nk Fau - Hulverson, M. A., et al. (2012). "Pharmacological characterization, structural studies, and in vivo activities of anti-Chagas disease lead compounds derived from tipifarnib." Antimicrob Agents Chemother.  56(9):4914-21.

PMID: 22777048 reprint  supp.

Pacrez-Moreno, G., Sealey-Cardona, M., Rodrigues-Poveda, C., Gelb, M. H., Ruiz-Pacrez, L. M., Castillo-Acosta, V., et al. (2012). "Endogenous sterol biosynthesis is important for mitochondrial function and cell morphology in procyclic forms of Trypanosoma brucei." International Journal for Parasitology, 42(11), 975-989.

PMID: 22964455. reprint

Crowther, G. J., Napuli, A. J., Gilligan, J. H., Gagaring, K., Borboa, R., Francek, C., Chen, Z., Dagostino, E. F., Stockmyer, J. B., Wang, Y., Rodenbough, P. P., Castaneda, L. J., Leibly, D. J., Bhandari, J., Gelb, M. H., Brinker, A., Engels, I. H., Taylor, J., Chatterjee, A. K., Fantauzzi, P., Glynne, R. J., Van Voorhis, W. C., Kuhen, K. L. (2011) "Identification of inhibitors of putative malaria drug targets among novel antimalarial compounds." Mol. Biochem. Parasitol., 175:21-29.  PMID:  20813141 reprint

Whitty, A., Gelb, M. H. (2010) "Critical challenges and emerging paradigms in drug discovery," Curr. Opin. Chem. Biol., 14(4):437-439.  PMID:  20621548   reprint

Kraus, J. M., Tatipaka, H. B., McGuffin, S. A., Chennamaneni, N. K., Karimi, M., Arif, J., Verlinde, L. M. J., Buckner, F. S., Gelb, M. H. (2010) "Second generation analogues of the cancer drug clinical candidate Tipifarnib for anti-Chagas disease drug discovery." J. Med. Chem., 53:3887-3898.  PMID:  20429511  reprint  supp.

Crowther, G. J., He, P., Rodenbough, P. P., Thomas, A. P., Kovzun, K. V., Leibly, D. J., Bhandari, J., Castaneda, L. J., Hol, W. G. J., Gelb, M. H., Napuli, A. J., Van Voorhis, W. C. (2010) "Use of thermal melt curves to assess the quality of enzyme preparations," Analytical Biochemistry, 399:268-275.  PMID:  20018159   reprint

Chennamaneni, N. K., Arif, J., Buckner, F. S. and Gelb, M. H. (2009) "Isoquinoline-based analogs of the cancer drug clinical candidate tipifarnib as anti-Trypanosoma cruzi agents," Bioorganic and Medicinal Chemistry Letters, 19:6582-6584.  PMID:  19875282  reprint  supp.

Suryadevara, P. K., Olepu, S., Lockman, J. W., Ohkanda, J., Karimi, M. Verlinde, C. L. M. J., Kraus, J. M., Schoepe, J., Van Voorhis, W. C., Hamilton, A. D., Buckner, F. S., and Gelb, M. H., (2009) "Structurally simple inhibitors of lanosterol 14alpha-demethylase are efficacious in a rodent model of acute Chagas disease," J. Med. Chem. 52:3703-3715.  PMID:  19463001 reprint supp.

Kraus, J. M., Verlinde, C. L. M. J., Karimi, Mandana, Lepesheva, G. I., Gelb, M. H., and Buckner, F. S. (2009) "Rational modification of a candidate cancer drug for use against chagas disease." J. Med Chem. 52:1639-1647.  PMID:  19239254 reprint sup sup

Torres-Santos, E. C., Sampaio-Santos, M. I., Buckner, F. S., Yokoyama, K., Gelb, M., Urbina, J. A., and Rossi-Bergmann, B. (2009) "Altered sterol profile induced in Leishmania amazonensis by a natural dihydroxymethoxylated chalcone," Journal of Antimicrobial Chemotherapy, 63:469-472. PMID:  19176591   reprint

Fan, E., Baker, D., Fields, S., Gelb, M. H., Buckner, F. S., Van Voorhis, W. C., Phizicky, E., Dumont, M., Mehlin, C., Grayhack, E., Sullivan, M., Verlinde, C., Detitta, G., Meldrum D. R., Merritt, E. A., Earnest, T., Soltis, M., Zucker, F., Myler, P. J., Schoenfeld, L., Kim, D., Worthey, L., Lacount, D., Vignali, M., Li, J., Mondal, S., Massey, A., Carroll, B., Gulde, S., Luft, J., Desoto, L., Holl, M., Caruthers, J., Bosch, J., Robien, M., Arakaki, T., Holmes, M., Le, Trong I., Hol, W. G. (2008) "Structural genomics of pathogenic protozoa: an overview" Methods Mol Biol., 426:497-513.   reprint

Ojo, Kayode K. , Gillespie, J. Robert, Reichers, Aaron J. , Napuli, Alberto J., Verlinde, Christophe L. M. J., Buckner, Frederick S., Gelb, Michael H., Domostoj, Mathias M., Wells, Susan J., Scheer, Alexander, Wells, Timothy N. C., and Van Voorhis, Wesley C. (2008) "Glycogen synthase kinase 3 is a potential drug target for African trypanosomiasis therapy," Antimicrobial Agents and Chemotherapy 52:3710-3717.  reprint.pdf

Mehlin, C., Boni, E., Buckner, F. S., Engel, L., Feist, T., Gelb, M. H., Haji, L., Kim, D., Liu, C., Mueller, N., Myler, P. J., Reddy, J. T., Sampson, J. N., Subramanian, E., Van Voorhis, W. C., Worthey, E., Zucker, F., Hol, W. G. (2006) "Heterologous expression of proteins from Plasmodium falciparum: results from 1000 genes,"
Mol Biochem Parasitol. 148:144-60. reprint.pdf

Eastman, R. T., Buckner, F. S., Yokoyana, K., Gelb, M. H. and Van Voorhis, W. C. (2006) "Thematic review series: lipid posttranslational modifications. Fighting parasitic disease by blocking protein farnesylation," J. Lipid Res. 47: 233-40 reprint.pdf

Gelb, M. H., Van Voorhis, W. C., Buckner, F. S., Yokoyama, K., Eastman, R., Carpenter, E. P., Panethymitaki, C., Brown, K. A., and Smith, D. F. (2003) "Protein farnesyl and N-myristoyl transferases: piggy-back medicinal chemistry targets for the development of antitrypanosomatid and antimalarial therapeutics," Molec. Biochem. Parasitol., 126:155-163. reprint.pdf

Choe, J., Suresh, S., Wisedchaisri, G., Kennedy, K. J., Gelb, M. H. and Hol, W. G. J. (2002) "Anomalous differences of light elements in determining precise binding modes of ligands to glycerol-3-phosphate dehydrogenase," Chem. Biol., 9:1189-1197. reprint.pdf

Gelb, M. H. and Hol, W. J. G. (2002) "Drugs to combat tropical protozoan parasites," Science, 297:343-344. reprint.pdf

Suresh, S., Bressi, J. C., Kennedy, K. J., Verlinde, C. L. M. J., Gelb, M. H., and Hol, W. G. J. (2001) "Conformational Changes in Leishmania mexicana Glyceraldehyde-3-phosphate Dehydrogenase Induced by Designed Inhibitors," J. Mol. Biol., 309:423-435. reprint.pdf

Bressi, J. C., Verlinde, C. L. M .J., Aronov, A. M., Shaw, M. L., Shin, S. S., Nguyen, L. N., Suresh, S., Buckner, F. S., Van Voorhis, W. C., Kuntz, I. D., Hol, W. G. J., and Gelb, M. H. (2001) "Adenosine Analogues as Selective Inhibitors of Glyceraldehyde-3-phosphate Dehydrogenase of Trypanosomatidae via Structure-Based Drug Design," J. Med. Chem, 44(13):2080-2093. reprint.pdf   supp.

Verlinde, C. L. M. J., Hannaert, V., Blonski, C., Willson, M., Perie, J. J., Fothergill-Gilmore, L. A., Opperdoes, F. R., Gelb, M. H., Hol, W. G. J., and Michels, P. A. M. (2001) "Glycolysis as a target for the design of new anti-trypanosome drugs," Drug Res. Updates, 4:1-14.reprint.pdf

Kennedy, K. J., Bressi, J. C., and Gelb, M. H. A Disubstituted NAD+ Analogue is a Nanomolar Inhibitor of Trypanosomal Glyceraldehyde-3-phosphate Dehydrogenase, Bioorg. Med. Chem. Lett., 11(2):95-98. reprint.pdf

Bressi, J. C., Choe, J., Hough, M. T., Buckner, F. S., Van Voorhis, W. C., Verlinde, C. L. M. J., Hol, W. G. J., Gelb, M. H. (2000) "Adenosine analogues as inhibitors of Trypanosoma brucei phosphoglycerate kinase: Elucidation of a novel binding mode for a 2-amino-N6-substituted adenosine," J. Med. Chem., 43:4135-4150. reprint.pdf

Golisade, A., Bressi, J. C., Van Calenbergh, S., Gelb, M. H., Link, A. Polymer-Assisted Solution-Phase Synthesis of 2'-Amido-2'-deoxyadenosine Derivatives Targeted at the NAD(+)-Binding Sites of Parasite Enzymes. J Comb Chem. 2000 Sep 12;2(5):537-544. reprint.pdf

Aronov, A. M.; Suresh, S., Buckner, F. S., Van Voorhis, W. C., Verlinde, C. L., Opperdoes, F. R., Hol, W. G., Gelb, M. H. Structure-based design of submicromolar, biologically active inhibitors of trypanosomatid glyceraldehyde-3-phosphate dehydrogenase. Proc Natl Acad Sci U S A. 96(8): 4273-8, 13 Apr 1999. reprint.pdf

Aronov, A. M., Verlinde, C. L., Hol, W. G., Gelb, M. H. Selective tight binding inhibitors of trypanosomal glyceraldehyde-3-phosphate dehydrogenase via structure-based drug design. J Med Chem. 41(24): 4790-9, Nov 1998. reprint.pdf
 
 

Clinical Enzymology and Quantitative Proteomics Reprints   back to top

Barcenas, M., Xue, C., Marushchak-Vlaskin, T., Scott, C. R., Gelb, M. H., Turecek, F. (2014) "Tandem mass spectrometry assays of palmitoyl protein thioesterase 1 and tripeptidyl peptidase activity in dried blood spots for the detection of Neuronal Ceroid Lipofuscinoses in newborns," Anal. Chem., July 14, 2014, DOI: 10.1021/ac501994b.  PMID: 25019629. reprint

Chennamaneni, N. K., Kumar, A. B., Barcenas, M., Specil, Z., Scott, C. R., Turecek, F., Gelb, M. H., (2014) "Improved reagents for newborn screening of Mucopolysaccharidosis types I, II, and VI by tandem mass spectrometry," Anal. Chem., 86:4508-4514, DOI: 10.1021/ac5004135.  PMID: 24694010.  reprint

Fitterer, B., Hall, P., Antonishyn, N., Desikan, R., Gelb, M., Lehotay, D. (2014) "Incidence and carrier frequency of Sandhoff disease in Saskatchewan determined using a novel substrate with detection by tandem mass spectrometry and molecular genetic analysis," Mol. Genet. Metab., 111:382-289.  doi: 10.1016/j.ymgme.2014.01.002.  PMID: 24461908.  reprint

Barcenas, M., Suhr, T. R., Scott, C. R., Turecek, F., Gelb, M. H. (2014) "Quantification of sulfatides in dried blood and urine spots from metachromatic leukodystrophy patients by liquid chromatography/electrospray tandem mass spectrometry," Clinica Chimica Acta, 433:39-43, PMID: 24370383.   reprint    supplemental material

Scott, C. R., Elliot, S., Buroker, N., Thomas, L. I., Keutzer, J., Glass, M., Gelb, M. H., Turecek, F. (2013) "Identification of infants at risk for developing Fabry, Pompe, or Mucopolysaccharidosis-I from newborn blood spots by tandem mass spectrometry." Journal of Pediatrics, 163:498-503. doi: 10.1016/j.jpeds.2013.01.013  PMID: 23465405. reprint

Orsini, J. J., Martin, M. M., Showers, A. L., Bodamer, O. A., Zhang, X. K., Gelb, M. H., et al. (2012). "Lysosomal storage disorder 4+1 multiplex assay for newborn screening using tandem mass spectrometry: Application to a small-scale population study for five lysosomal storage disorders." Clinica Chimica Acta, 413(15-16), 1270-1273.

PMID:  22548856.  reprint

Spacil, Z., Tatipaka, H., Barcenas, M., Scott, C. R., Turecek, F., Gelb, M. H. (2012) "High-throughput assay of 9 lysosomal enzymes for newborn screening." Clinical Chemistry.,  59 (3), 1530-8561.  PMID: 23315484.  reprint

Wolf, B. J., Ghomashchi, F., Kim, T., Abam, C. A., Sadilek, M., Jack, R., Thompson, J. N., Scott, C. R., Gleb, M. H., Turecek, F. (2012) "New substrates and enzyme assays for the detection of mucopolysaccharidosis III (Sanfilippo Syndrome) types A, B, C, and D by tandem mass spectrometry," Bioconjug Chem., 23:557-564.  PMID: 22372747, PMC: 3310265 reprint supp

Spacil, Z., Hui, R., Gelb, M. H., & Turecek, F. (2011). "Protonation sites and dissociation mechanisms of t-butylcarbamates in tandem mass spectrometric assays for newborn screening." Journal of Mass Spectrometry, 46(10), 1089-1098. PMID: 22012676, PMC3212097  reprint

Spacil, Z., Elliott, S., Reeber, S. L., Gelb, M. H., Scott, C. R. and Turecek, F. (2011) "Comparative triplex tandem mass spectrometry assays of lysosomal enzyme activities in dried blood spots using fast liquid chromatography:  Application to newborn screening of  Pompe, Fabry, and Hurler diseases" Anal. Chem., 83:4822-4828.  PMID: 21548611, PMC 3115456 reprint supp.

Wolfe, B. J., Blanchard, S., Sadilek, M., Scott, C. R., Turecek, F., Gelb, M. H. (2011) "Tandem mass spectrometry for the direct assay of Lysosomal enzymes in dried blood spots:  Application to screening newborns for Mucopolysaccharidosis II (Hunter Syndrome)" Anal. Chem., 83:1152-1156.  PMID:  21192662   reprint supp.

Khaliq, T., Sadilek, M., Scott, C. R., Turecek, F., Gelb, M. H. (2011) "Tandem mass spectrometry for the direct assay of lysosomal enzymes in dried blood spots:  Application to screening newborns for Mucopolysaccharidosis IVA," Clin. Chem., 57(1):128-131.  PMID: 21030685   reprint  supp.

Duffey, T. A., Sadilek, M., Scott, C. R., Turecek, F., Gelb, M. H. (2010) "Tandem mass spectrometry for the direct assay of lysosomal enzymes in dried blood spots:  Application to screening newborns for Mucopolysaccharidosis VI (Maroteaux-Lamy Syndrome)", Anal. Chem., 82:9587-9591.  PMID:  20961069   reprint  supp.

Choiniere, J. R., Scott, C. R., Gelb, M. H., Turecek, F. (2010) "Direct assay of delta-aminolevulinic acid dehydratase in heme biosynthesis for the detection of porphyrias by tandem mass spectrometry," Anal. Chem., 82:6730-6736.  PMID:  20583792 reprint  supp.

Duffey, T. A., Bellamy, G., Elliott, S., Fox, A. C., Glass, M., Turecek, F., Gelb, M. H., Scott, C. R. (2010) "A tandem mass spectrometry triplex assay for the detection of Fabry, Pompe, and Mucopolysaccharidosis-I (Hurler)," Clin Chem., 56(12):1854-1861.  PMID: 20876176   reprint supp.

Zhang, X. K., Elbin, C. S., Turecek, F., Scott, R., Chuang, W. L., Keutzer, J. M., Gelb, M. (2010) "Multiplex lysosomal enzyme activity assay on dried blood spots using tandem mass spectrometry," Methods Mol. Biol., 603:339-350.  PMID:  20077085  reprint

Duffey, T. A., Khaliq, T., Scott, C. R., Turecek, F., Gelb, M. H. (2010) "Design and synthesis of substrates for newborn screening of Maroteaux-Lamy and Morquio A syndromes," Bioorg. Med. Chem. Lett., 20(20):5994-5996.   PMID:  20833037 reprint  supp.

Blanchard, S., Turecek, F., Gelb, M. H. (2009) "Short synthetic sequence for 2-sulfation of alpha-L-iduronate glycosides" Carbohydrate Research,  344:1032-1033.  PMID:  19356745  reprint supp.

Blanchard, Sophie, Sadilek, Martin, Scott, C. Ronald, Turecek, Frantisek, and Gelb, Michael H. (2008) "Tandem mass spectrometry for the direct assay of Lysosomal enzymes in dried blood spots:  Application to screening newborns for Mucopolysaccharidosis I," Clin. Chem., 54:2067-2070.  reprint   supplemental data

Wang, Y., Scott, C. R., Gelb, M. H., Turecek, F. (2008) "Direct Assay of Enzymes in Heme Biosynthesis for the Detection of Prophyrias by Tandem Mass Spectrometry," Anal. Chem., 80:2606-2611. reprint.pdf  supp.

Wang, Y., Gatti, P., Sadilek, M., Scott, C. R., Turecek, F., Gelb, M. H. (2008) "Direct Assay of Enzymes in Heme Biosynthesis for the Detection of Porphyrias by Tandem Mass Spectrometry. Uroporphyrinogen Decarboxylase and Coproporphyrinogen III Oxidase," Anal. Chem., 80:2599-2605.   reprint.pdf  supp.

Turecek, F., Scott, C. R., Gelb, M. H. (2007) “Tandem mass spectrometry in the detection of inborn errors of metabolism for newborn screening,” Methods Mol. Biol., 359:143-157. reprint.pdf

Lu, Y., Bottari, P., Aebersold, R., Turecek, F., Gelb, M. H. (2007) “Absolute quantification of specific proteins in complex mixtures using visible isotope-coded affinity tags,” Methods Mol. Biol., 359:159-176. reprint.pdf

Wang, D., Wood,T., Sadilek, M., Scott, C. R., Turecek, F. and M. H. Gelb  (2007) "Tandem mass spectrometry for the direct assay of enzymes in dried blood spots: Application to newborn screening for Mucopolysaccharidosis II (Hunter Disease)," Clin. Chem. 53:137-140.    reprint.pdf  supp.

Gelb, M. H., Turecek, F., Scott, R. C., and Nestor, A. Chamoles. (2006) " Direct multiplex assay of enzymes in dried blood spots by tandem mass spectrometry for the newborn screening of lysosomal storage disorders," J Inherit Metab Dis 29:397–404. reprint.pdf

Seebacher, J., Mallick, P., Zhang, N., Eddes J. S., Aebersold, R. and Gelb, M. H. (2006)" Protein cross-linking analysis using mass spectrometry, isotope-coded cross-linkers, and integrated computational data processing." Journal of Proteome Research, 5(9):2270-82.  reprint.pdf

Scott, C. R. (2005) "Newborn screening for lysosomal storage disorders,"

Clinical Perspectives 12:11-15. reprint.pdf

Wang, D., Eadala, B., Sadilek, M., Chamoles N. A., Turecek, F., Scott, C. R. and Gelb, M. H. (2005) "Tandem mass spectrometric analysis of dried blood spots for screening of Mucopolysaccharidosis I in newborns," Clin. Chem. 51:898-900. reprint.pdf, supplement.pdf, editorial.pdf

Li, Y., Scott, C. R., Chamoles, N. A., Ghavami, A., Pinto, B. M., Turecek, F., Gelb, M. H. (2004) "Direct multiplex assay of lysosomal enzymes in dried blood spots for newborn screening," Clin. Chem., 50:1785-96. reprint.pdf  supp.

Lu, Y., Bottari, P., Turecek, F., Aebersold, R. and Gelb, M. H. (2004) "Absolute quantification of specific proteins in complex mixtures using visible isotope-coded affinity tags," Anal. Chem., 76:4104-4111. reprint.pdf  supp.

Li, Y., Brockman, K., Turecek, F., Scott, C. R., Gelb, M. H. (2004) "Tandem mass spectrometry for the direct assay of enzymes in dried blood spots: Application to newborn screening for Krabbe Disease," Clin. Chem., 50: 638-640. reprint.pdf   data supplement.pdf

Bottari, P., Aebersold, R., Turecek, F., and Gelb, M. H. (2004) "Design and synthesis of visible isotope-coded affinity tags for the absolute quantification of specific proteins in complex mixtures," Bioconj. Chem., 15:380-388.  reprint

Li, Y., Ogata, Y., Freeze, H. H., Scott, C. R., Turecek, F., and Gelb, M. H. (2003) "Affinity capture and elution/electrospray ionization mass spectrometry assay of phosphomannomutase and phosphomannose isomerase for the multiplex analysis of congenital disorders of glycosylation types Ia and Ib," Anal. Chem., 75:42-48. reprint.pdf  supp.

Ogata, Y., Scampavia, L., Ruzicka, J., Scott, C. R., Gelb, M. H., and Turecek, F. (2002) "Automated affinity capture-release of biotin-containing conjugates using a lab-on-valve apparatus coupled to UV/Visible and electrospray ionization mass spectrometry," Anal. Chem., 74:4702-4708. reprint.pdf

Gerber, S. A., Turecek, F., and Gelb, M. H. (2001)  "Design and synthesis of substrate and internal standard conjugates for profiling enzyme activity in the Sanfilippo Syndrome by affinity chromatography/electrospray ionization mass spectrometry." Bioconj. Chem., 12(4): 603-315. reprint.pdf

Zhou, X., Turecek, F., Scott, C. R., and Gelb, M. H. "Quantification of cellular acid sphingomyelinase and galactocerebroside B-galactosidase activities by electrospray ionization mass spectrometry". Clin. Chem., 2001, 47(5):874-881. reprint.pdf

Gerber, S. A., Scott, C. R., Turecek, F., Gelb, M. H. "Direct profiling of multiple enzyme activities in human cell lysates by affinity chromatography/electrospray ionization mass spectrometry: Application to clinical enzymology". Anal. Chem., 2001, 73:1651-1657. reprint.pdf

Gygi, S. P, Rist, B., Gerber, S. A., Turecek, F., Gelb, M. H, Aebersold R. "Quantitative analysis of complex protein mixtures using isotope-coded affinity tags". Nat Biotechnol. 1999 Oct;17(10):994-9 reprint.pdf commentary.pdf

Gerber, S. A., Scott, C. R., Turecek, F., Gelb, M. H. (1999) "Analysis of rates of muliple enzymes in cell lysates by electrospray ionization mass spesctrometery," J. Am. Chem. Soc. 121:1102-1103; 121:176.  reprint  supp.
 
 

Other Reprints   back to top

Siegel, J. B., Zanghellini, A., Lovick, H. M., Kiss, G., Lambert, A. R., St. Clair, J. L., Gallaher, J. L., Hilvert, D., Gelb, M. H., Stoddard, B. L., Houk, K. N., Michael, F.E., Baker, D. (2010) "Computational design of an enzyme catalyst for a stereoselective bimolecular Diels-Alder reaction," Science, 329:309-313.  PMID:  20647463 reprint

Chipuk, J .E., Gelb, M. H., Brodbelt, J. S. (2010) "Rapid and selective screening for sulfhydryl analytes in plasma and urine using surface-enhanced transmission mode desorption electrospray ionization mass spectrometry," Anal. Chem,82:4130-4139.  PMID:  20402469  reprint

Trinh, K., Andrews, L., Krause, J., Hanak, T., Lee, D., Gelb, M. and Pallanck, L. (2010) "Decaffeinated coffee and nicotine-free tobacco provide neuroprotection in drosophila models of Parkinson's Disease through an NRF2-dependent mechanism," J Neurosci, 30:5525-5532.  PMID:  20410106   reprint

Chipuk, J. E., Gelb, M. H., Brodbelt, J. S. (2010) "Surface-enhanced transmission mode desorption electrospray ionization:  Increasing the specificity of ambient ionization mass spectrometric analyses," Anal. Chem. 82:16-18.  PMID:  19902914   reprint

Holt, A., Koehorst, R. B., Rutters-Meijneke, T., Gelb, M. H., Rijkers, D. T., Hemminga, M. A., Killian, J. A. (2009) "Tilt and rotation angles of a transmembrane model peptide as studied by fluorescence spectroscopy," Biophys. J., 97:2258-2266.  PMID:  19843458  reprint

Gelb, M. H. and Holm, B. P. (2006) "A simple and inexpensive device for removal of solvent from a large collection of sample tubes," J Comb Chem. 8:15-27. reprint.pdf

Kuksa, V., Bartl, F., Maeda, T., Jang, G.-F., Ritter, E., Heck, M., Van Hooser, J. P., Liang, Y., Filipek, S., Gelb, M. H., Hofmann, K. P., and Palczewski, K., "Biochemical and physiological properties of rhodopsin regenerated with 11-cis-6-ring- and 7-ring-retinals". J. Biol. Chem., 2002, 277:42315-42324. reprint.pdf

Goldstein, A. S., Gelb, M. H., and Yager, P. "The relationship between the structure of the headgroup of sphingolipids and their ability to form complex high zxial ratio microstructures". Chemistry and Physics of Lipids, 2001, 109:1-14. reprint.pdf

Goldstein, A. S., Gelb, M. H., and Yager, P. "Continuous and highly variable rate controlled release of model drugs from sphingolipid-based complex high axial ratio microstructures". J. of Controlled Release, 2001, 70:125-138. reprint.pdf

Jang, G. F., Kuksa, V., Filipek, S., Bartl, F., Ritter, E., Gelb, M. H., Hofmann, K. P., and Palczewski, K., "Mechanism of rhodopsin activation as examined with ring constrained retinal analogs and the crystal structure of the ground state protein".  J. Biol. Chem., 2001, 276: 26148-26153. reprint.pdf

Stecher, H., Gelb, M. H., Saari, J. C., Palczewski, K., "Preferential release of 11-cis-retinol from retinal pigment epithelial cells in the presence of cellular retinaldehyde-binding protein". J Biol Chem. 274(13): 8577-85, 26 Mar 1999. reprint.pdf