Interrogating the Translated Transcriptome

TSAA. Traditional approaches to genome-wide expression analysis measure total transcript levels. This is valuable information, but it gives no insight into the rates of synthesis of the proteins encoded by a transcriptome. We developed Translation State Array Analysis or TSAA, which allows an estimate of the loading of ribosomes onto the mRNAs of a transcriptome as well as transcript levels. We have used this approach to interrogate several biological systems, including responses of yeast to mating pheromone and cellular stresses, gene expression in differentiating ES cells and translational control upon macrophage attachment to a substratum. See Morris (2009) for a discussion of an exciting new approach to exploration of translation state.

RiboTag. A major issue in genome biology is how to examine gene expression in the individual cells in a tissue. The RiboTag mouse provides a powerful tool for interrogating the ribosome-associated transcriptomes of single cell types in complex tissues such as brain. The approach is readily applied to any cell type for which a specific Cre recombinase driver is available.