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Colocalization of GFAP and fura-2 in the Neonatal Rat Optic Nerve (RON). Top left, Confocal image of GFAP immunoreactivity in a Postnatal day 1 (P1) RON. Cy3 secondary antibody was excited at 550 nm, and the image was collected at 565 nm. This slice was taken at the edge of the nerve (oriented at the top of the panel), showing a number of GFAP+ somata. Top right, Confocal image of fura-2 in the same section as the top left panel. Fura-2 was excited at 364 nm, and the image was collected at 520 nm. Bottom, Superimposed images from the top panels, showing that all GFAP+ cells contain fura-2. |