Baneyx BioNano Laboratories.
..Publications

.Journal Articles
Baneyx, F. and J.F. Matthaei. 2014. "Self-assembled two-dimensional protein arrays in bionanotechnology: from S-layers to designed lattices". Curr. Opin. Biotechnol. 28:39-45.
Zhou, W., A.O. Moguche, D. Chiu, K. Murali-Krishna and F. Baneyx. 2013. "Just-in-time vaccines: Biomineralized calcium phosphate core-immunogen shell nanoparticles induce long-lasting CD8+ T cell responses in mice. Nanomedicine. In press.
Zhou, W. and F. Baneyx. 2013. "Biofabrication of ZnS:Mn luminescent nanocrystals using histidine, hexahistidine, and His-tagged proteins: a comparison study". Biochem. Eng. J. In press.
Coyle, B.L., M. Rolandi and F. Baneyx. 2013. "Carbon-binding designer proteins that discriminate between sp2- and sp3-hybridized carbon surfaces". Langmuir 29:4839-4846

Baneyx, F. and J.-K. Park. 2013. "Editorial: Nanobio versus bionano - What's in a name?" Biotechnol. J. 8:158-159.

Nannenga, B.L. and F. Baneyx. 2012. "Folding engineering strategies for efficient membrane protein production in E. coli". Methods Mol. Biol. 899:187-202.
Kitayaporn, S., W. Zhou, D.T. Schwartz and F. Baneyx. 2012. "Laying out ground rules for protein-aided nanofabrication: ZnO synthesis at 70oC as a case study" Biotechnol. Bioeng. 109:1912-1918.
Baneyx, F. "A ribosomal surprise". 2012. Biotechnol. J. 7:326-327.
Sedlak, R.H., M. Hnilova, C. Grosh, H. Fong, F. Baneyx, D.T. Schwartz, M. Sarikaya, C. Tamerler, B. Traxler. 2012. " An engineered Escherichia coli silver-binding periplasmic protein promotes silver tolerance". Appl. Environ. Microbiol. 78:2289-2296.
Chiu, D., W. Zhou, S. Kitayaporn, D.T. Schwartz, K. Murali-Krishna, T.J. Kavanagh and F. Baneyx. 2012. "Biomineralization and size control of stable calcium phosphate core-protein shell nanoparticles: potential for vaccine applications" Bioconjug. Chem. 23:610-617.
Nannenga, B.L. and F. Baneyx. 2011. "Enhanced expression of membrane proteins in E. coli with a PBAD promoter mutant: synergies with chaperone pathway engineering strategies". Microb. Cell Fact. 10:105.
Zhou, W. and F. Baneyx, F. 2011. "Aqueous, protein-driven synthesis of transition metal-doped ZnS immuno-quantum dots". ACS Nano 5:8013-8018.
Nannenga, B.L. and F. Baneyx. 2011. "Reprogramming chaperone pathways to improve membrane protein expression in Escherichia coli". Protein Sci. 20:1411-1420.
Baneyx, F. 2011. "Review of biomimetic and bioinspired nanomaterials". J. Am. Chem. Soc. 133:5161.
Baneyx, F. and B.L. Nannenga. 2010. "Chaperones: a story of thrift unfolds". Nat. Chem. Biol. 6:880-881.
Puertas, J.M., B.L. Nannenga, K.T. Dornfeld, J.M. Betton and F. Baneyx. 2010. "Enhancing the secretory yields of leech carboxypeptidase inhibitor in Escherichia coli: influence of trigger factor and signal recognition particle". Protein Express. Purif. 74:122-128.
Kitayaporn, S., J.H. Hoo, K. Böhringer, F. Baneyx and D.T. Schwartz. 2010. "Orchestrated structure evolution: accelerating direct-write nanomanufacturing by combining top-down patterning with bottom-up growth". Nanotechnology 21:195306.
Zhou, W., D.T. Schwartz and F. Baneyx. 2010. "Single pot biofabrication of zinc sulfide immuno-quantum dots". J. Am. Chem. Soc. 132:4731-4738.
Grosh, C., D.T. Schwartz and F. Baneyx. 2009. "Protein-based control of silver growth habit using electrochemical deposition". Cryst. Growth Des. 9:4401-4406.
Sastry, M.S.R., W. Zhou and F. Baneyx. 2009. "Integrity of N- and C-termini is important for E. coli Hsp31 chaperone activity". Protein Sci. 18:1439-1447.
Allred, D.B., A. Cheng, M. Sarikaya, F. Baneyx and D.T. Schwartz. 2008. "3-D architecture of inorganic nanoarrays electrodeposited through an S-layer protein mask". Nano Lett. 8:1434-1438.
Sengupta, A., C.K. Thai, M.S.R. Sastry, J.F. Matthaei, D.T. Schwartz, E.J. Davis and F. Baneyx. 2008. "A genetic approach for controlling the binding and orientation of proteins on nanoparticles". Langmuir 24:2000-2008.
Shapiro, E. and F. Baneyx. 2007. "Stress-activated bioluminescent E. coli sensors for antimicrobial agents detection". J. Biotechnol. 132:487-493.
Choe, W.-S., M.S.R. Sastry, C.K. Thai, H. Dai, D.T. Schwartz and F. Baneyx. 2007. "Conformational control of inorganic adhesion in a designer protein engineered for cuprous oxide binding". Langmuir 23:11347-11350.
Baneyx, F. and D.T. Schwartz. 2007. "Selection and analysis of solid-binding peptides". Curr. Opin. Biotechnol. 18:312-317.
Allred, D.B., M. Sarikaya, F. Baneyx and D.T. Schwartz. 2007. "Bacterial surface-layer proteins for electrochemical nanofabrication". Electrochim. Acta. 53:193-199.
Presenda, A., D.B. Allred, D.B., F. Baneyx, D.T. Schwartz and M. Sarikaya. 2007. "Stability of S-layer proteins for electrochemical nanofabrications". Colloids Surf. B. 57:256-261.
Mujacic, M. and F. Baneyx. 2007. "Chaperone Hsp31 contributes to acid resistance in stationary phase Escherichia coli". Appl. Environ. Microbiol. 73:1014-1018.
Allred, D.B., M.T. Zin, H. Ma, M. Sarikaya, F. Baneyx, A.K.-Y. Jen and D.T. Schwartz. 2007. "Direct nanofabrication and TEM analysis on a suite of easy-to-prepare ultrathin film substrates". Thin Solid Films 515:5341-5347.
Mujacic, M. and F. Baneyx. 2006. "Regulation of Escherichia coli hchA, a stress-inducible gene encoding molecular chaperone Hsp31". Mol. Microbiol. 60:1576-1589.
Shapiro, E., C. Lu and F. Baneyx. 2005. "A set of multicolored Photinus pyralis luciferase mutants for in vivo bioluminescence applications". Protein Eng. Des. Sel. 18:581-587 (cover story).
Dai, H., W.-S. Choe, C.K. Thai, M. Sarikaya, B. Traxler, F. Baneyx and D.T. Schwartz. 2005. "Nonequilibrium synthesis and assembly of hybrid inorganic-protein nanostructures using an engineered DNA-binding protein" J. Am. Chem. Soc. 127:15637-15643.
Chow, I.-T., M.E. Barnett, M. Zolkiewski and F. Baneyx. 2005. "The N-terminal domain of Escherichia coli ClpB enhances chaperone function". FEBS Lett. 579:4242-4248.
Chow, I.-T.and F. Baneyx. 2005. "Coordinated synthesis of the two ClpB isoforms improves the ability of Escherichia coli to survive thermal stress". FEBS Lett. 579:4235-4241.
Allred, D.B., M. Sarikaya, F. Baneyx and D.T. Schwartz. 2005. "Electrochemical nanofabrication using crystalline protein masks". Nano Lett. 5:609-613 (cover story; cover art selected for commemorative poster of the 230th American Chemical Society Annual Meeting).
Baneyx, F. and M. Mujacic. 2004. "Recombinant protein folding and misfolding in Escherichia coli". Nat. Biotechnol. 22:1399-1408.
Thai, C.K., H. Dai, M.S.R. Sastry, M. Sarikaya, D.T. Schwartz and F. Baneyx. 2004. "Identification and characterization of Cu2O and ZnO binding polypeptides by Escherichia coli cell surface display: towards an understanding of metal oxide binding". Biotechnol. Bioeng. 87:129-137.
Sastry, M.S.R., P. Quigley, W.G.J. Hol and F. Baneyx. 2004. "The linker-loop region of E. coli chaperone Hsp31 functions as a thermal gate that modulates high affinity substrate binding at elevated temperatures". Proc. Natl. Acad. Sci. USA 101:8587-8592.
Baneyx, F. 2004. "Keeping up with protein folding". Microb. Cell Fact. 3:6.
Sarikaya, M., C. Tamerler, D.T. Schwartz, and F. Baneyx. 2004. "Materials assembly and formation using engineered polypeptides". Annu. Rev. Mat. Res. 34:373-408.
Dai, H., C.K. Thai, M.Sarikaya, F. Baneyx, and D. T. Schwartz. 2004. "Through-mask anodic patterning of copper surfaces and film stability in biological media". Langmuir 20:3483-3486 (cover story).
Mujacic, M., M. Bader and F. Baneyx. 2004. "Escherichia coli Hsp31 functions as a holding chaperone that cooperates with the DnaK-DnaJ-GrpE system in the management of protein misfolding under severe stress conditions". Mol. Microbiol. 51:849-859.
Quigley, P., K. Korotkov, F. Baneyx and W.G.J. Hol. 2004. "A new native EcHsp31 structure suggests key role of structural flexibility for chaperone function". Protein Sci. 13:269-277.
Sarikaya, M., C. Tamerler,  A.K.-Y. Jen, K. Schulten and F. Baneyx. 2003. "Molecular biomimetics: nanotechnology through biology". Nat. Mater. 2:577-585 (cover story and Thomson ISI highly cited Fast Breaking Paper Dec. 2004).
Quigley, P., K. Korotkov, F. Baneyx and W.G.J. Hol. 2003. "The 1.6 Å crystal structure of the class of chaperones represented by E. coli Hsp31 reveals a putative catalytic triad". Proc. Natl. Acad. Sci. USA 100:3137-3142.
Amatore, D. and F. Baneyx. 2003. "Insertion mutagenesis of Escherichia coli GroEL". Biochem. Biophys. Res. Commun. 302:246-252.
Baneyx, F and J.L Palumbo. 2003. "Improving heterologous protein folding via molecular chaperone and foldase co-expression". Methods Mol. Biol. 205:171-197.
Baneyx, F and M. Mujacic. 2003. "Cold-inducible promoters for heterologous protein expression". Methods Mol. Biol. 205:1-18.
Sastry, M.S.R., K. Korotkov, Y. Brodsky and F. Baneyx. 2002. "Hsp31, the Escherichia coli yedU gene product, is a molecular chaperone whose activity is inhibited by ATP at high temperatures". J. Biol. Chem. 277:46026-46034.
Shapiro, E and F. Baneyx. 2002. "Stress-based identification and classification of antibacterial agents: second generation Escherichia coli reporter strains and optimization of detection". Antimicrob. Agents Chemother. 46:2490-2497.
Cooper, K.W. and F. Baneyx. 2001. "Escherichia coli FtsH (HflB) degrades a membrane-associated TolAI-II-beta-lactamase fusion protein under highly denaturing conditions". Protein Express. Purif. 21:323-332.
Thomas, J.G and F. Baneyx. 2000. "ClpB and HtpG facilitate de novo protein folding in stressed Escherichia coli  cells". Mol. Microbiol. 36:1360-1370.
Bianchi, A.D. and F. Baneyx. 1999. "Hyperosmotic shock induces the sigma32 and sigmaE stress regulons of Escherichia coli". Mol. Microbiol. 34:1029-1038.
Bianchi, A.D. and F. Baneyx. 1999. "Stress responses as a tool to detect and characterize the mechanism of action of antibacterial agents". Appl. Environ. Microbiol. 65:5023-5027 (Journal Highlight ASM News [2000] 66:28).
Baneyx, F. 1999. "Recombinant protein expression in Escherichia coli". Curr. Opin. Biotechnol. 10:411-421.
Mujacic, M., Cooper, K.W. and F. Baneyx. 1999. "Cold-inducible cloning vectors for low  temperature protein expression in Escherichia coli: application to the production of a toxic and proteolytically sensitive fusion protein". Gene 238:325-332.
Shearstone, J.R. and F. Baneyx. 1999.  "Biochemical characterization of the small heat shock protein IbpB from Escherichia coli". J. Biol. Chem. 274:9937-9945.
Wan, E.W.M. and F. Baneyx. 1998. "TolAIII co-overexpression facilitates the recovery of periplasmic recombinant proteins into the growth medium of Escherichia coli". Protein Express. Purif. 14:13-22.
Thomas, J.G. and F. Baneyx. 1998. "Roles of the Escherichia coli small heat shock proteins IbpA and IbpB in thermal stress management: comparison with ClpA, ClpB, and HtpG in vivo". J. Bacteriol. 180:5165-5172.
Vasina, J.A., M.S. Peterson and F. Baneyx. 1998. "Scale-up and optimization of the low-temperature-inducible cspA promoter system". Biotechnol. Prog. 14:714-721.
Thomas, J.G. and F. Baneyx. 1997. "Divergent effects of chaperone overexpression and ethanol supplementation on inclusion body formation in recombinant Escherichia coli". Protein Express. Purif. 11:289-296.
Thomas, J.G., A. Ayling and F. Baneyx. 1997. "Molecular chaperones, folding catalysts and the recovery of biologically active recombinant proteins from E. coli: to fold or to refold". Appl. Biochem. Biotech. 66:197-238.
Schneider, E.L., J.G. Thomas, J.A. Bassuk, E.H. Sage and F. Baneyx. 1997. "Manipulating the aggregation and oxidation of human SPARC in the cytoplasm of Escherichia coli". Nat. Biotechnol. 15:581-585.
Vasina, J.A. and F. Baneyx. 1997. "Expression of aggregation-prone recombinant proteins at low temperatures: a comparative study of the E. coli cspA and tac promoter systems". Protein Express. Purif. 9:211-218.
Bassuk, J.A., L.P. Braun, K. Motamed, F. Baneyx and E.H. Sage. 1996. "Renaturation of secreted protein acidic and rich in cysteine (SPARC) expressed in Escherichia coli requires isomerization of disulfide bonds for recovery of biological activity". Int. J. Biochem. Cell Biol. 28:1031-1043.
Thomas, J.G. and F. Baneyx. 1996. "Protein folding in the cytoplasm of Escherichia coli: requirements for the DnaK-DnaJ-GrpE and GroEL-GroES molecular chaperone machines". Mol. Microbiol. 21:1185-1196.
Thomas, J.G. and F. Baneyx. 1996. "Influence of a global deregulation of the heat-shock response on the expression of aggregation-prone proteins in Escherichia coli". Ann. New York Acad. Sci. 782:478-485.
Thomas, J.G. and F. Baneyx. 1996. "Protein misfolding and inclusion body formation in Escherichia coli.cells overexpressing heat shock proteins". J. Biol. Chem. 271:11141-11147.
Vasina, J.A. and F. Baneyx. 1996. "Recombinant protein expression at low temperatures under the transcriptional control of the major E. coli cold shock promoter cspA". Appl. Environ. Microbiol.  62:1444-1447.
Ayling, A. and F. Baneyx. 1996. "Influence of the GroE molecular chaperone machine on the in vitro refolding of Escherichia coli beta-galactosidase". Protein Sci. 5:478-487.
Bassuk, J.A., F. Baneyx, R.B. Vernon, S.E. Funk and E.H. Sage. 1996. "Expression of biologically active human SPARC in Escherichia coli". Arch. Biochem. Biophys. 325:8-19.
Baneyx, F., U. Bertsch, C.E. Kalbach, S.M. van der Vies, J. Soll and A.A. Gatenby. 1995. "Spinach chloroplast cpn21 co-chaperonin possesses two functional domains fused together in a toroidal structure, and exhibits nucleotide-dependent binding to plastid chaperonin 60". J. Biol. Chem. 270:10695-10702.
Baneyx, F. 1994. "Chaperonins and protein folding". Ann. New York Acad. Sci. 745:383-394.
Baneyx, F. and G. Georgiou. 1992. "Degradation of secreted proteins in Escherichia coli". Ann. New York Acad. Sci. 665:301-308.
Baneyx, F. and A.A. Gatenby. 1992. "A Mutation in GroEL interferes with protein folding by reducing the rate of discharge of sequestered polypeptides". J. Biol. Chem. 267:11637-11644.
Bowden, G.A., F. Baneyx and G. Georgiou. 1992. "Abnormal fractionation of beta-lactamase in Escherichia coli: evidence for an interaction with the inner membrane in the absence of a leader peptide". J. Bacteriol. 174:3407-3410.
Baneyx, F., A. Ayling, T. Palumbo, D. Thomas, and G. Georgiou. 1991. "Optimization of growth conditions for the production of proteolytically-sensitive proteins in the periplasmic space of Escherichia coli". Appl. Microbiol. Biotechnol. 36:14-20.
Baneyx, F. and G. Georgiou. 1991. "Construction and characterization of Escherichia coli strains deficient in multiple secreted proteases: protease III degrades high molecular weight substrates in vivo". J. Bacteriol. 173:2696-2703.
Georgiou, G. and F. Baneyx. 1990. "Expression, purification and immobilization of a protein A-beta-lactamase hybrid protein". Ann. New York Acad. Sci. 589:139-147.
Baneyx, F., C. Schmidt and G. Georgiou. 1990. "Affinity immobilization of a genetically engineered bifunctional hybrid protein". Enzyme Microb. Technol. 12:337-342.
Baneyx, F. and G. Georgiou. 1990. "In vivo degradation of secreted fusion proteins by the Escherichia coli outer membrane protease OmpT". J. Bacteriol. 172:491-494.
Baneyx, F. and G. Georgiou. 1989. "Expression, purification and enzymatic characterization of a protein A-beta-lactamase hybrid protein". Enzyme Microb. Technol. 11:559-567.
TOP
.Book Chapters
Coyle, B.L., W. Zhou and F. Baneyx. 2013. "Protein-aided mineralization of inorganic nanostructure". In Bionanotechnology: Biological Self-Assembly and its Applications. Rehm, B.H.A. Ed, Horizon Scientific Press, pp 63-83.
Mujacic, M. and F. Baneyx. 2004. "Expression, folding and degradation in Escherichia coli". In Protein Expression Technologies: Current Status and Future Trends, Baneyx, F. Ed, Horizon Bioscience, Wymondham, United Kingdom, pp 85-148.
Baneyx, F. 1999. "In vivo folding of recombinant proteins in Escherichia coli".  In Manual of Industrial Microbiology and Biotechnology, 2nd edition, Davies J.E., A.L. Demain, G. Cohen, C.L. Hershberger, L.J. Forney, I.B. Holland, W.-S. Hu, J.-H.D. Wu, D.H. Sherman, R.C. Wilson  Eds, ASM Press, Washington D.C., pp 551-565.
Baneyx, F. and A.A. Gatenby. 1993. "GroEL-mediated protein folding".  In Protein Folding In Vivo and In Vitro.  Cleland, J.L. Ed. ACS Press, Washington D.C., pp 133-141.
Gatenby, A.A., G.K. Donaldson, F. Baneyx, G.H. Lorimer, P.V. Viitanen and S.M. van der Vies. 1993. "The participation of GroE heat shock proteins in polypeptide folding". In Biocatalyst Design for Stability and Specificity.  Himmel, M.E. and Georgiou, G. Eds, ACS Press, Washington D.C., pp 140-150.
Baneyx, F. and G. Georgiou. 1992. "Expression of proteolytically sensitive proteins in E. coli". In Stability of Protein Pharmaceuticals: Chemical and Physical Paths of Protein Degradation. Ahren, T.J. and Manning, M.C. Ed., Plenum Press, N.Y., pp 69-108.
TOP
.Books
Baneyx, F (Ed). 2004. Protein Expression Technologies: Current Status and Future Trends. Horizon Biosciences, Wymondham, United Kingdom. 532 pp.
Lucotte, G. and F. Baneyx.  1993. Introduction to Molecular Cloning Techniques. VCH Publishers, NY 298 pp.
TOP
.E-media
Baneyx, F. 2007. "Hsp31: a general stress protein of Escherichia coli". In Molecular Chaperones: Principles and Diseases, Houry, W. Ed, Henry Stewart Talks.
Baneyx, F. 2007. "Amino acids and proteins". National Nanotechnology Infrastructure Network Open Textbook.
Baneyx, F. 2007. "Sugars and polysaccharides". National Nanotechnology Infrastructure Network Open Textbook.
Baneyx, F. 2007. "Lipids". National Nanotechnology Infrastructure Network Open Textbook.
TOP
.Patents
Baneyx, F, A.A. Gatenby and C.E. Kalbach. "A method for enhancing the production of biologivally active recombinant proteins" WO 94/23042
Baneyx, F, A.A. Gatenby and C.E. Kalbach. "Process for enhancing the production of heterologous protein in biologically active conformation in a transformed E. coli dnaK mutant host cell" US 5,552,301.
Georgiou, G. and F. Baneyx. "Method of producing proteolytically sensitive proteins". US 5,508,192.
Georgiou, G. and F. Baneyx. "Protease-deficient strains for production of proteolytically sensitive proteins". US 5,264,365.
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Contact: François Baneyx, University of Washington, Department of Chemical Engineering, Box 351750, Seattle, WA Tel: 206-685-7659 Fax: 206-685-3451 E-mail: baneyx@uw.edu

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