Direct Random Mutagenesis of Gene-Sized DNA Fragments Using Polymerase Chain Reaction
	Analytical Biochemistry 224, 347-353 (1995)
	Concentrations (mM) and error rates
	E. (%)	[dATP]	[dCTP]	[dGTP]	[dTTP]	[MnCl2]	[MgCl2]
	0.2	0.35	0.4	0.20	1.35	0.5	2.50
	0.3	0.20	0.2	0.18	1.26	0.5	2.04
	0.4	0.22	0.2	0.27	1.86	0.5	2.75
	0.5	0.22	0.2	0.34	2.36	0.5	3.32
	0.6	0.23	0.2	0.42	2.90	0.5	3.95
	0.8	0.23	0.2	0.57	4.00	0.5	5.20
	1.0	0.12	0.1	0.36	2.50	0.5	3.28
	1.5	0.12	0.1	0.55	3.85	0.5	4.82
	PCR Setup
		amount in ul:
	E. (%)	H2O	dATP	dCTP	dGTP	dTTP	Templ.	Pri.	BSA	MgCl2	Buffer	MnCl2	Enz.	total
	0.2	54.2	3.5	4.0	2.0	1.4	2	2		10.0	10.0	10.0	1	100.0
	0.3	54.8	2.0	2.0	1.8	1.3	2	2	5	8.2	10.0	10.0	1	100.0
	0.4	50.2	2.2	2.0	2.7	1.9	2	2	5	11.0	10.0	10.0	1	100.0
	0.5	46.8	2.2	2.0	3.4	2.4	2	2	5	13.3	10.0	10.0	1	100.0
	0.6	42.8	2.3	2.0	4.2	2.9	2	2	5	15.8	10.0	10.0	1	100.0
	0.8	35.2	2.3	2.0	5.7	4.0	2	2	5	20.8	10.0	10.0	1	100.0
	1.0	48.6	1.2	1.0	3.6	2.5	2	2	5	13.1	10.0	10.0	1	100.0
	1.5	39.2	1.2	1.0	5.5	3.9	2	2	5	19.3	10.0	10.0	1	100.0
	E. (%)	H2O	dATP	dCTP	dGTP	dTTP	Templ.	Pri.	BSA	MgCl2	Buffer	MnCl2	Enz.	total
	stock solution conc. (mM)
	dATP	10
	dCTP	10
	dGTP	10
	dTTP	100
	MnCl2	5
	MgCl2	25
	BSA	0.1	ug/ul
	Total Volume		100	ul