Curriculum Vitae

Anitra E. Ingalls                                                                    March 12, 2009
Assistant Professor                                                                e-mail: aingalls@u.washington.edu
School of Oceanography
Box 355351
Seattle, WA  98195-5351

Phone (206) 221-6748

Education

Ph.D.

Coastal Oceanography. May 2002. Stony Brook University, Marine Sciences Research Center, Stony Brook, NY. Dissertation: The Role of Biogenic Carbonate and Silicate in the Preservation of Organic Matter.

M.S.

Marine Environmental Science. January 1998.
Stony Brook University, Marine Sciences Research Center, Stony Brook, NY.

B.A.

Chemistry (minor in Biochemistry). May 1992.
Reed College, Portland, OR.

Professional Experience

Assistant Professor, School of Oceanography, University of Washington. September, 2004 - Present.
Postdoctoral Fellow, Department of Earth and Planetary Sciences, Harvard University. January 2003-July 2004.
Postdoctoral Research Scientist, Lamont-Doherty Earth Observatory of Columbia University. Associate, Department of Earth and Planetary Sciences, Harvard University, July, 2002 - Dec. 2002.
Graduate Research Assistant, Stony Brook University, MSRC. 1996-2002.
Research Staff Assistant, Lamont-Doherty Earth Observatory of Columbia University 1992-1995.
Supervisors: Drs. James K. Bishop (1992-1995) and Robert Anderson (1995).
Research Assistant, Reed College. Supervisor: Arthur Glasfeld, Summer 1991.

Fellowships and Honors

Fellowships Hess Postdoctoral Fellowship (declined). Princeton University. Department of Geosciences. March 2002
Kavli Fellow, National Academy of Sciences, November, 2009

Research Interests

  • Intact polar lipid analysis for microbial fingerprinting
  • Biomarkers for bacterial and archaeal ammonia oxidation
  • Bacterial and archaeal biomarkers as tracers for terrestrial organic matter
  • Archaeal lipids as paleotemperature proxies
  • Archaeal lipids in hot springs and hydrothermal environments
  • Archaeal lipids in cultured isolates, including thermophiles.
  • Characterization of novel diatom-bound organic compounds
  • Protection of organic matter by biominerals in the water column and sediment.
  • Paleoceanographic investigations of mineral-bound organic matter.
  • LC-MS methods for isolating individual compounds for isotopic (14/12C, 13/12C, 15/14N) analysis:
    • Radiocarbon dating intact polar lipid biomarkers in water column particulate matter and sediments to investigate the role of marine Archaea and Bacteria in the cycling of carbon and nitrogen in the marine environment
    • Radiocarbon analysis of individual organic compounds in diatom frustules to determine sediment age and depositional history
    • Development of new LC-MS methods for purification of compounds for compound-specific radiocarbon analysis - lignin phenols, mycosporine-like amino acids and fatty acids

Invited Presentations

National Academy of Sciences, Kalvi Frontiers in Science Symposium, Beckman Center, UC Irvine.  Invited poster presenter.  November 2009.  Microbial metabolisms of the ocean past and present as seen through the lens of biomarkers.
Gordon Research Conference, Marine Microbes. Tilton, NH, Invited for July. 2010.
Gordon Research Conference, Chemical Oceanography. Tilton, NH Aug. 2009. Bridging the gap between microbial ecology and geochemistry: ammonia oxidizers and their biomarkers, abundance and activity.
Goldschmidt Conference. May 2009, Davos Switzerland.  Declined invitation.
Unknown Knowns and Known Unknowns: Chemical Oceanography in a Changing World. A symposium on the future of chemical oceanography in a changing world.  Feb, 2009.  Savannah GA. What can biomarkers of archaea tell us about the carbon cycle?
Oregon Graduate Institute CMOP Distinguished Lecture Series. Beaverton, OR.  December 6, 2007. Terrestrial organic carbon in the sea: A multi-proxy investigation.
University of California Santa Cruz, Ocean Sciences Department Seminar, October 18, 2006.  Membrane lipids of planktonic archaea: from metabolic pathways to biogeochemistry.
University of Washington, School of Oceanography, Bio Lunch Seminar. May 16, 2006. Quantifying archaeal community autotrophy using natural radiocarbon. 
Stanford University, Oceans Seminar, March 14, 2006, Understanding microbial metabolisms in the mesopelagic ocean using natural radiocarbon.
University of Washington, Department of Civil and Environmental Engineering, Stahl Lab, October 7, 2005. Quantifying Archaeal Autotrophy in the Mesopelagic Ocean Using Natural Radiocarbon.
Oregon State University, School of Oceanography, November 11, 2004, Diatom Frustule-Bound Organic Matter as a Paleoproxy: Progress and Pitfalls.
Harvard University, Department of Earth and Planetary Sciences.  February 22, 2003, Earth and Planetary Sciences Day.  Applications of LC-MS in Compound-Specific Radiocarbon Studies of the Marine Carbon Cycle.
Symposium on Biogeochemical Cycles.  August 28, 2003.  Friday Harbor Laboratory, Washington.  A tribute to Prof. John I. Hedges.  Invited speaker. Biomineral-Bound Organic Matter:  From Carbon Preservation to Paleoceanography.
University of Washington, School of Oceanography.  Chemical Oceanography, June 5, 2003. Applications of Preparative LC-MS in Compound-Specific Isotope Studies:  Diatoms and Archaea.
University of Washington, School of Oceanography.  Departmental Seminar, June 4, 2003.  Selective Preservation and Paleoceanographic Potential of Biomineral-Bound Biomarkers.
Woods Hole Oceanographic Institution, Department of Chemistry and Geochemistry.  March 18, 2003.  Diatom-Bound Organic Matter:  From Carbon Preservation to Paleoceanography.
Princeton University, Department of Geosciences. February 2002.  The Role of Biominerals in the Preservation of Organic Carbon.

Presentations

AGU/ASLO Ocean Sciences Meeting, February, 2010, Portland, OR.  The potential of diatom frustule-bound long chain polyamines as indicators of environment, species and cell status.  A. E. Ingalls; M. C. Bridoux.
AGU/ASLO Ocean Sciences Meeting, February, 2010, Portland, OR.  HPLC purification of higher plant-derived lignin phenols for radiocarbon dating.  E. E. Ellis; A. E. Ingalls; K. McDuffee; R. G. Keil; G. M. dos Santos; E. R. Druffel.
AGU/ASLO Ocean Sciences Meeting, February, 2010, Portland, OR. Multi-proxy evidence for changes over the last 125 years in the input of terrestrial organic matter to an anoxic fjord: Tofino Inlet, Vancouver Island, British Columbia. K. McDuffee; R. G. Keil; A. E. Ingalls.
Gordon Research Conference, Organic Geochemistry.  August 2008.  Holderness NH.  Poster: The living fraction of marine and terrestrial glycerol dialkyl glycerol tetraethers. Ingalls AE and Huguet C.
Gordon Research Conference, Organic Geochemistry.  August 2006.  Holderness NH.  Poster: Quantifying archaeal autotrophy in the mesopelagic ocean using natural radiocarbon. Ingalls AE, Shah S, Hansman RL, Aluwihare LI, Santos GM, Druffel ERM, Pearson A.
AGU/ASLO Ocean Sciences Meeting, February, 2006, Honolulu, HI. Talk: Quantifying archaeal autotrophy in the mesopelagic ocean using natural radiocarbon. Ingalls AE, Shah S, Hansman RL, Aluwihare LI, Santos GM, Druffel ERM, Pearson A.
AMS-10, September 2005, University of California, Berkeley, CA. Talk: A compound-specific approach to dating the organic template of sedimentary diatom frustules. Ingalls AE, Pearson A, Anderson RF, Guilderson T.
Gordon Research Conference. Chemical Oceanography August, 2005. Tilton, NH.  Poster: Archaeal metabolisms in the "Twilight Zone".  Ingalls AE, Pearson A., Shah, S, Hansman RI, Aluwihare, LI, Santos GM, Griffin S, and Druffel ERM.
Gordon Research Conference. Chemical Oceanography August, 2003. Tilton, NH.  Poster: Characterizing and Radiocarbon dating Diatom-Bound Organic Matter.  Ingalls AE, Anderson RF, Pearson A.
JGOFS Open Science Meeting. May 2003. National Academy of Sciences. Washington DC.  Poster Session Chair. Poster presenter.  Awarded travel expenses.
AGU/EGS/EUG Joint Assembly.  April 2003.  Nice, France. Poster:  Prospects for radiocarbon dating diatom-bound organic compounds.  Ingalls AE, Anderson RF, Pearson A, Lee C.
AGU/ASLO. February 2002. Honolulu, HI.  Poster: Organic Matter Fluxes and Preservation in the Southern Ocean: Role of Diatoms. Ingalls AE, Lee C, Hedges JI & Wakeham SG.
Gordon Research Conference. Chemical Oceanography. August, 2001. Tilton School, Tilton, NH.  Poster: The Role of Biominerals in the Sinking Flux and Preservation of Amino Acids in the Southern Ocean along 170°W. Ingalls AE, Lee C, Hedges JI & Wakeham SG.
Gordon Research Conference. Organic Geochemistry.  August, 2000. Holderness School, Plymouth, NH.  Poster: Organic Carbon Preservation in the Southern Ocean. Ingalls AE, Lee C, Hedges JI & Wakeham SG.
AGU/ASLO. February, 2000. San Antonio, TX. Talk: Organic Carbon Preservation in Southern Ocean Sediments. Ingalls AE, Lee C, Aller RC, Hedges JI, & Wakeham SG.
Gordon Research Conference.  Chemical Oceanography.  August, 1999.  Kimbel Union Academy, Meriden, NH.  Poster:  The Role of Biogenic Minerals in Organic Carbon Preservation.  Ingalls AE, Lee C, Aller RC, Wakeham SG & Hedges JI.
ASLO Spring Meeting.  February, 1999.  Santa Fe, NM.  Poster: Organic Carbon in Coral and Carbonate Sediments.  Ingalls AE, Lee C & Aller RC.
AGU/ASLO.  February, 1998.  San Diego, CA.  Poster: The Influence of Macrofauna on Chlorophyll-a Degradation in Coastal Marine Sediments.  Ingalls AE, Aller RC, Lee C & Sun M-Y.
Chesapeake Region Association of Biogeochemists, Third Annual Meeting.  May, 1996. UVA, Charlottesville, VA.  Talk:  Particle Mixing Coefficients and Chlorophyll-a in the Cape Hatteras Shelf Break.  Ingalls AE, Lee C & Aller RC.

Other Presentations (presenter's name is italicized)

AGU/ASLO Ocean Sciences Meeting, February, 2010, Portland, OR. HPLC purification of higher plant-derived lignin phenols for radiocarbon dating: E E Ellis, A E Ingalls, K McDuffee, R G Keil, G M dos Santos, E R Druffel.

AGU/ASLO Ocean Sciences Meeting, February, 2010, Portland, OR. Multi-proxy evidence for changes over the last 125 years in the input of terrestrial organic matter to an anoxic fjord: Tofino Inlet, Vancouver Island, British Columbia: K McDuffee, R G Keil, A E Ingalls.

1st International Conference on Nitrification ICoN1, July 2009.  University of Louisville, KY, Hotspots of Archaeal nitrification in coastal marine environments.  Poster. Urakawa H, Martens-Habbena W, Ingalls AE, Huguet C, de la Torre J, Stahl D.

International Meeting of Organic Geochemistry, September, 2009. Bremen, Germany. Talk. A matter of live and dead.  Huguet C and Ingalls AE.

Unknown knowns and Known Unknowns: Chemical Oceanography in a Changing World. Feb, 2009.  Savannah GA.  Poster: 14C and 13C characteristics of suspended populations in an oligotrophic water column. Close H, Druffel ERM, Eglinton TI, Dos Santos G, Ingalls AE and Pearson A.

AGU Fall Meeting, December 2008. San Francisco, CA. Atmospheric modulation of western Antarctic Peninsula Holocene climate variability: Insights from the Palmer Deep TEX86 record.  Shevenell AE, Domack EW, Ingalls AE.

Gordon Research Conference, Organic Geochemistry.  August 2008.  Holderness NH.  Poster: 14C and 13C characteristics of suspended populations in an oligotrophic water column. Close H, Druffel ERM, Eglinton TI, Dos Santos G, Ingalls AE and Pearson A.

Gordon Research Conference, Organic Geochemistry.  August 2008.  Holderness NH.  Poster: Analytical window of opportunity. Huguet C and Ingalls AE.

Gordon Research Conference, Organic Geochemistry.  August 2008.  Holderness NH.  Poster: Multi-proxy evidence for recent changes in the input of terrestrial organic matter to an anoxic fjord: Tofino Inlet, Vancouver, BC. McDuffee K and Ingalls AE and Keil RG.

10th International Symposium on Antarctic Earth Sciences. UC Santa Barbara.  August 2007.  Orbital and atmospheric forcing of western Antarctic Peninsula climate in the Holocene: The TEX86 paleotemperature record of Palmer Deep. Shevenell AE, Ingalls AE and Domack EW.

ALSO Aquatic Sciences Meeting. Santa Fe, NM, February 2007. Talk.  Estimating the importance of terrestrial inputs to lake ecosystems in southwest Alaska using the BIT index and lignin phenols. S. Alin, S.R., Schindler, D.E., Ingalls, A.E.

AGU Fall Meeting, December 2006. San Francisco, CA. Invited Talk: Atmospheric forcing of Holocene climate variability along the western Antarctic Peninsula: Novel insights from the TEX86 paleotemperature record of Palmer Deep.  Shevenell AE, Ingalls AE, Domack EW, Emerson SR.  EOS Trans., AGU, 87 (47), Fall Meet. Suppl., Abstract PP02-0648AGU.

Gordon Research Conference, Organic Geochemistry.  August 2006.  Holderness NH.  Poster: Microbial membrane lipids as tracers for terrestrial organic carbon in coastal sediments.  Walsh EM, Ingalls AE, Keil RG, dos Santos GM, Druffel RM.

WPGM Western Pacific Geophysics Meeting: Geomicrobiological Processes in Extreme Environments. July, 2006.  Beijing China.  Lipid biomarker evidence of microbially mediated methane and non-methane hydrocarbon oxidation in the Gulf of Mexico.  Pi Y, Pancost R, Ingalls AE, Pearson A, Zhang C.

ASM American Society for Microbiology Annual, Meeting May 2006 Orlando, FL. Hydrocarbon effects on the distribution of archaeal lipids in the Gulf of Mexico: Implications for deep-subsurface supported microbial populations. Pi Y, Pancost R, Ingalls AE, Pearson A, Zhang C.

AGU/ASLO Ocean Sciences Meeting, February, 2006, Honolulu, HI. Talk:  Testing the BIT index: Spatial and Temporal Variability in Sources of Organic Carbon to Vancouver Island Margin Sediments as Evidenced by Ether Lipids from Bacterial and Archaeal Membranes.  Walsh EM, Ingalls AE, Keil RG. (Best student paper award).

AGU/ASLO Ocean Sciences Meeting, February, 2006, Honolulu, HI. Poster. Repeat Hydrographic and Sediment Surveys in Barkley, Clayoquot and Nootka Sounds (British Columbia,  Canada) between 2000-2005.  Greengrove C,  Keil R, Chin-Leo G, Logsdon M, Ingalls AE, Nuwer J, Walsh E, Delwiche L Evans C.

AMS-10, September 2005, University of California, Berkeley, CA.  Exploring the minimum sample size limit for CSRA: lessons from the water column. Pearson A, Ingalls AE, Shah SR, Hansman RL, Aluwihare LI, dos Santos G, Druffel ERM.

ASLO Summer Meeting, June 2005.  Santiago de Compostela, Spain. Metabolic capabilities of prokaryotes in the deep ocean. Hansman RI, Aluwihare LI, Pearson A, Shah, S Ingalls, AE.

Goldschmidt Conference, May, 2005.  Moscow Idaho.  Metabolic capabilities of prokaryotes in the deep ocean.  Hansman RI, Aluwihare LI, Pearson A, Shah, S Ingalls, AE.

Publications

McDuffee K, Ingalls AE and Keil R In prep, A history of environmental change in a Vancouver Island fjord.  K. McDuffee is co-advised by myself and R. Keil.  Keil and McDuffee came up with this project.  I supervised the lab work that took place in my lab, have had numerous discussion about the data with co-authors and have read drafts of the manuscript.

Urakawa H, Martens-Habbena W, Cash A, Huguet C, Ingalls AE, Devol A, Stahl DA, In prep, Fjords as hot spots for Archaeal nitrification. PNAS.

Ingalls AE, McDuffee K, Ellis E, Keil R, dos Santos Guaciara, Druffell ERM. In prep. A new method for radiocarbon dating higher plant derived lignin phenols using HPLC purification.  Analytical Chemistry.

Huguet C, Truxal L and Ingalls AE.  In prep. Distribtution of archaea among size fractionated particulate organic matter elucidated from intact and core membrane lipids in a fjord. AEM.

Shevenell A, Ingalls AE, Domack E, Orbital and atmospheric forcing of western Antarctic Peninsula climate during the Holocene: The Tex86 temperature record of Palmer Deep. In review, Nature.

Bridoux M Neilson S., and Ingalls AE, In revision. The occurrence and application of long chain polyamines in sedimentary diatom frustules, a major component of diatom-bound organic matter. GCA.

Huguet C, Martens-Habbena W, Urakawa H, Truxal L, Stahl DA, Ingalls AE.  In revision. Changes in intact membrane lipid content of archaeal cells may indicate metabolic status. Organic Geochemistry.

Huguet C, Martens-Habbena W, Urakawa H, Stahl DA, Ingalls AE. In Press. Optimization of extraction methods for intact and core glycerol dialkyl glycerol tetraethers (GDGTs). 2010. L&O Methods.

Ingalls AE, Whitehead KI, Bridoux, M.  2010.  Tinted windows:  the presence and identification of the UV-absorbing compounds, mycosporine-like amino acids, in the frustules of marine diatoms.  Geochimica Cosmochimica Acta.  doi 10.1016/j.gca.2009.09.012.

Hansman RL, Griffin S, Watson JT, Druffel ERM, Ingalls AE, Pearson A, Aluwihare LI. 2009. The radiocarbon signature of microbial organisms in the mesopelagic ocean. PNAS, 106(16) 6513-6518.

Schouten, S, Hopmans EC, van der Meer J, Mets A, Bard E, Bianchi TS, Diefendorf A, Escala M, Freeman KH, Furukawa Y, Huguet C, Ingalls AE, Ménot-Combes G, Nederbragt AJ, Oba M, Pearson A, Pearson EJ, Rosell-Melé A, Schaeffer P, Shah SR, Shanahan TM, Smith RW, Smittenberg R, Suzuki M, Talbot HM, Uchida M, Van Mooy BAS, Yamamoto M, Zhang Z, Sinningh Damsté J. 2009. An interlaboratory study of TEX86 and BIT analysis using high performance liquid chromatography/mass spectrometry. G-Cubed, 10(3) doi:10.1029/2008GC002221.

de la Torre JR, Walker CB, Ingalls AE, Könneke M, Stahl DA. 2008. Cultivation of a thermophilic ammonia oxidizing archaeon synthesizing crenarchaeol. Environmental Microbiology. doi 10.1111/j1462-2920. 

Walsh E. Ingalls AE, Keil R. 2008. Sources and Transport of Terrestrial Organic Matter in Vancouver Island Fjords and the Vancouver- Washington Margin:  A multiproxy approach using δ13Corg, lignin phenols and the ether lipid BIT Index. Limnology and Oceanography. 53(3) 1054-1063.

Shevenell, A.E., Ingalls, A.E., and E.W. Domack, 2007. Orbital and atmospheric forcing of western Antarctic Peninsula climate in the Holocene: The TEX86 paleotemperature record of Palmer Deep. Changing World-Online Proceedings of the 10th ISAES X, A.K. Cooper and C.R. Raymond et al., eds., USGS Open-File Report, p. 131-4p, vol. 1047. 

Ingalls AE, Liu ZF and Lee C. 2006.  Seasonal and latitudinal trends in source and diagenesis of sinking particles in the Pacific sector of the Southern Ocean: insights from amino acids, pigments and diatom-bound organic matter. Deep-Sea Research I.

Ingalls AE, Shah S, Hansman RL, Aluwihare LI, Santos GM, Druffel ERM, Pearson A. 2006.  Quantifying archaeal community autotrophy in the mesopelagic ocean using natural radiocarbon. 103: 6442-6447. PNAS.

Ingalls AE and Pearson A. The Oceanography Society, Ten years of Compound-Specific Radiocarbon Analysis.  Invited article, Oceanography.  September, 2005, 12-25.

Ingalls AE, Anderson RF, Pearson A, 2004. Compound-specific radiocarbon dating of diatom-bound organic compounds. Marine Chemistry. 92:  91-105.

Ingalls AE, Lee C and Aller RC. 2004.  Organic matter diagenesis in shallow water carbonate sediments. Geochimica et Cosmochimica Acta. 68: 4363-4379

Pearson A., Huang Z., Ingalls A. E., Romanek C., Wiegel J. Freeman K. H., Smittenberg R. H. and Zhang C.L.  2004. Non-marine crenarchaeol in Nevada hot springs, Applied and Environmental Microbiology.  70: 9, 5229-5237.

Ingalls AE, Lee C and Druffel ERM. 2003.  Organic matter preservation in the skeletons of two mound-forming corals.  Geochimica Cosmochimica et Acta.  67: 4, 2827-2841.

Ingalls AE, Lee C, Hedges JI and Wakeham SG. 2003.  The role of biominerals in the sinking flux and preservation of amino acids in the Southern Ocean along 170°W.  Deep-Sea Research II.  50:3-4, 709-734.

Ingalls AE, Aller RC, Lee C and Sun M-Y.  2000. The influence of macrofauna on chlorophyll-a degradation in coastal marine sediments.  Journal of Marine Research 58:4, 631-651.

Ph.D. and Postdoctoral advisors

Cindy Lee (SUNYSB), Robert C. Aller (SUNYSB), Robert F. Anderson (LDEO) and Ann Pearson (Harvard University)

Teaching

University of Washington: Introduction to Ocean Lab (201), Spring 2008, 2009, 2010.
University of Washington: Marine Organic Geochemistry (522), Winter, 2008, 2010.
Maternity leave: Part time (teaching release) Spring 2007.
Maternity leave: Winter 2007.
University of Washington: Marine Organic Geochemistry (522), Spring, 2006.
University of Washington: Marine Chemistry (400), Winter, 2006.
University of Washington: Marine Organic Geochemistry (522), Spring, 2005.
SUNY: Lecturer/TA Chemical Oceanography, (Graduate), Spring, 1998.
SUNY: Teaching Assistant, Introduction to Oceanography, Spring, 1996.
SUNY: Laboratory Instructor, Introduction to Chemistry, Fall, 1995.
Reed: Tutor, Introduction to Chemistry, Fall, 1991.
Reed:  Laboratory Instructor, Organic Chemistry, 1990-1991.

Professional Affiliations

American Society of Limnology and Oceanography, 1995-present
American Geophysical Union, 1995-present
American Chemical Society
The Oceanography Society

Postdoctoral Advisees

Maxime Bridoux (paid on NSF Chemical Oceanography grant)
Carme Huguet (paid on NSF Biological Oceanography grant)
Amelia Shevenell (PCC Fellow, joint with Steve Emerson) now at U. College London

Postdoctoral collaborators who did projects in my lab

Simone Alin (Post doc with Jeff Richey and Daniel Schindler) now at NOAA

Students

Elizabeth Walsh – Masters candidate, (NDSEG Fellow)
Kelsey McDuffee –Masters candidate (NSF Fellow)
Erin Ellis – PhD candidate (NSF Fellow, co-advised with Jeff Richey)
Celia Kelly – Undergraduate research assistant
Britta Voss – Undergraduate advisee
Laura Spencer Hollaway – undergraduate research assistant –SURP 2008