| 1 |
Folch, A. and Toner, M. "Microengineering of Cellular Interactions", Annual Rev. of Biomedical Engineering 2, 227 (2000). |
|
|
This paper is a review that covers microfabrication techniques used to modulate cell-substrate, cell-cell, and cell-medium interactions. |
||
| 2 | Tourovskaia, A., Barber, T., Wickes, B., Hirdes, D., Grin, B., Castner, D. G., Healy, K. E., and Folch, A. "Micropatterns of Chemisorbed Cell Adhesion-Repellent Films Using Oxygen Plasma Etching and Elastomeric Masks", Langmuir 19, 4754 (2002). |
![]() |
|
This paper reports a method to confine cells for many days to micropatterns of proteins adsorbed on glass. |
||
| 3 | Chen, C., Hirdes, D., and Folch, A. "Gray-Scale Photolithography Using Microfluidic Photomasks", Proceedings of National Academy of Sciences 100, 1499 (2003) --> see feature in the New York Times, Materials Today, Science News, Physics World, Photonics Spectra, Biophotonics International, and Physics Web. |
![]() |
| This paper reports the development of photolithographic masks that contain fluidic features of tunable opacity, yielding unique 3D microstructures. | ||
| 4 | Li, N., Tourovskaia, A., and Folch, A. "Biology on a Chip: Microfabrication in Cell Culture Studies", Critical Reviews in Biomedical Engineering 31, 68 (2003). | |
| This paper reviews work on cell culture studies that incorporate microfabrication in order to modulate the microenvironment of cells. | ||
| 5 | Neils, C. M., Tyree, Z., Finlayson, B., and Folch, A. "Combinatorial Mixing of Microfluidic Streams", Lab on a Chip 4, 342 (2004). |
![]() |
| In this paper we describe a microfluidic mixer that outputs all the sixteen combinations of four titrations of two dyes in continuous flow. | ||
| 6 | Hsu, C.-H., Chen, C., and Folch, A. "'Microcanals' for Micropipette Access to Single Cells in Microfluidic Environments", Lab on a Chip 4, 420 (2004) --> featured in the RSC's Chemical Biology Virtual Journal (2004, Iss. 19). |
![]() |
| This paper reports the first implementation of open-air microfluidic channels and their use to probe single cells with micropipettes within a microfluidic environment. | ||
| 7 | Hoffman, J., Shao, J., Hsu, C.-H., and Folch, A. "Elastomeric Molds with Tunable Microtopographies", Advanced Materials 16, 2201 (2004). |
![]() |
| This paper reports a micromolding method based on molds whose microtopography can be tuned by the user, thereby producing features that are difficult or impossible to obtain by traditional photolithography. | ||
| 8 | Tourovskaia, A., Figueroa-Masot, X. and Folch, A., "Differentiation-on-a-chip: A Microfluidic Platform for Long-Term Cell Culture Studies", Lab on a Chip 5, 14 (2005) --> cited in the cover and featured in Sample Content (thus available free of charge). |
![]() |
| This paper demonstrates the first 2-week-long cell culture in a (optionally, heterogeneous) microfluidic environment. | ||
| 9 | Hsu, C.-H., Chen, C., and Folch, A. "Microfluidic Devices with Tunable Microtopographies", Applied Physics Letters 86, 023508 (2005) --> featured in the Virtual Journal of Nanoscale Science & Technology (Vol. 11, Iss. 2) and in the Virtual Journal of Biological Physics Research (Vol. 9, Iss. 2). |
![]() |
| This paper implements our tunable-microtopography technique inside a microfluidic channel to demonstrate novel micromixers and fluid traps. | ||
| 10 | Keenan, T.M., Hooker, A., Spilker, M. E., Boggy, G. J., Li, N., Vicini, P., and Folch, A. "Automated Identification of Axonal Growth Cones in Time-Lapse Image Sequences", J. Neurosci. Methods 151, 232 (2005). |
![]() |
| This paper reports an image recognition software to track and quantitate the growth of axons from sequences of time-lapse phase-contrast micrographs of neurons growing on a flat substrate. | ||
| 11 | Rettig, J.R. and Folch, A. "Large-Scale Single-Cell Trapping and Imaging Using Microwell Arrays", Analytical Chemistry 77, 5628 (2005). |
![]() |
| This article demonstrates the use of microwells to trap single cells in large arrays. | ||
| 12 | Kosar,
T.F., Chen, C., Stucky, N.L., and Folch, A. "Arrays of Microfluidically-Addressable Nanoholes", Journal of Biomedical Nanotechnology 1, 161 (2005). |
|
| This paper reports the fabrication of nanoholes on silicon nitride membranes to stimulate cells from underneath the cell culture surface. | ||
| 13 | Stucky, N.L., Chen, C., Kosar, T.F., and Folch, A. "Fabrication of Microfluidically-Accessible Planar Nanoholes on Elastomeric Substrates", Journal of Biomedical Nanotechnology 1, 384 (2005). | ![]() |
| This paper reports the fabrication of nanoholes on silicon nitride membranes to stimulate cells from underneath the cell culture surface. | ||
| 15 | Li, N., Hsu, C.-H., and Folch, A. "Parallel mixing of Photolithographically-Defined Nanoliter Volumes Using Elastomeric Microvalve Arrays", Electrophoresis 26, 3758 (2005). |
|
| This article demonstrates the use of microvalve arrays to create multiple titrations simultaneously (demonstration of a calcium-sensitive dye calibration). | ||
| 16 | Li, N. and Folch, A. "Integration of topographical and biochemical cues by axons during growth on microfabricated 3-D substrates", Experimental Cell Research 311, 307 (2005). |
![]() |
| This paper reports effects of the microscale substrate composition and topography on axon growth in cultured embryonic cortical neurons. | ||
| 17 | T.F. Kosar, Tourovskaia, A., Figueroa-Masot, X., Adams, M., and Folch, A. "A Nanofabricated Planar Aperture as a Mimic of the Nerve-Muscle Contact During Synaptogenesis", Lab Chip 6, 632 (2006). --> featured in Chemical Biology (top-viewed article in May 2006) and in the Faculty of 1000 Biology. |
![]() |
| This article demonstrates that nanofluidic delivery of soluble agrin to myotubes induces local clustering of acetylcholine receptors. | ||
| 18 | Tourovskaia, A., T.F. Kosar, and Folch, A. "Local Induction of Acetylcholine Receptor Clustering in Myotube Cultures Using Microfluidic Application of Agrin", Biophysical Journal 90, 2192 (2006). |
![]() |
| This paper shows the local clustering of acetylcholine receptors on selected areas of cultured myotubes using microfluidic focal application of agrin. | ||
| 19 | Frevert, C.W., Boggy, G., Keenan, T.M., and Folch, A. "Measurement of Cell Migration in Response to an Evolving Radial Chemokine Gradient Triggered by a Microvalve", Lab on a Chip 6, 849 (2006) --> cited in the cover. |
![]() |
| In this paper we use a microfluidic valve to create chemotactic gradients and obtain neutrophil migration measurements correlated with spatiotemporal gradient values. | ||
| 20 | Keenan, T.M., Hsu, C.-H., and Folch, A. "Microfluidic "Jets" for Generating Steady-State Gradients of Soluble Molecules on Open Surfaces", Applied Physics Letters 89, 114103 (2006) --> featured in the Virtual Journal of Nanoscale Science & Technology (Vol. 14, Iss. 13) and in the Virtual Journal of Biological Physics Research (Vol. 12, Iss. 6). |
|
| In this paper we demonstrate the use of microfluidic "jets" to create dynamic gradients of soluble molecules on open surfaces. | ||
| 21 | Tourovskaia, A., Figueroa-Masot, X. and Folch, A., "Long-term Microfluidic Cultures of Myotube Microarrays for High-Throughput Focal Stimulation ", Nature Protocols 1, 1092 (2006). |
![]() |
| In this paper we report the precise protocols for culturing and focally stimulating single-myotube microarrays in a microfluidic device. | ||
| 22 |
Chen, C. and Folch, A., "A High-Performance Elastomeric Patch Clamp Chip", Lab on a Chip (2006), 6, 1338. |
![]() |
| We report a microfluidic patch clamp chip that allows for performing whole-cell recordings with unprecedented yields and exchanges of the extracellular and intracellular solutions. | ||
| 23 | Hsu, C.-H. and Folch, A., "Spatiotemporally- Complex Concentration Profiles Using a Tunable Chaotic Micromixer", Applied Physics Letters 89, 144102 (2006) --> featured in the Virtual Journal of Nanoscale Science & Technology (Vol. 14, Iss. 16). |
|
| We demonstrate the generation of temporal sequences of complex concentration profiles using a single device containing tunable microtopographies. | ||
| 24 | Lam, E.W., Cooksey, G.A., Finlayson, B.A., and Folch, A., "Microfluidic Circuits with Tunable Flow Resistances", Applied Physics Letters (2006), 89, 164105 (2006). |
![]() |
| In this paper we present the concept and implementation of a microfluidic "resistor" (a segment of a microchannel with user-controlled flow resistance). | ||